An evaluation of Lux technology as an alternative methodology to determine growth rates of Listeria in laboratory media and complex food matrices

Nyhan, Laura; Begley, Máire; Johnson, Nicholas; Callanan, Michael

doi:10.1016/j.ijfoodmicro.2019.108442

Cited by 6 publications

(2 citation statements)

References 41 publications

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“…Previous studies have demonstrated the effectiveness of fluorescence for detecting microbial growth, such as the growth of Acinetobacter johnsonii in domestic wastewater sludge samples (Rossetti et al, 2007). Similarly, the bioluminescence or fluorescence technology was used by Nyhan et al (2020) and Krishnamurthi et al (2021) as an alternative method to determine the μmax of Listeria spp. and E. coli K-12 (MG1655) strain encoding the GFP protein, respectively.…”

Section: Discussionmentioning

confidence: 99%

The role of temperature and carbon dioxide climatic stress factors on the growth kinetics ofEscherichia coli

Roufou

Griffin

Attard

et al. 2023

Journal of Applied Microbiology

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Aims The global level of carbon dioxide and temperature in the atmosphere is expected to increase, which may affect the survival of the stress-adapted bacteria. In this study, the effect of temperature and dissolved carbon dioxide on the growth rate of Escherichia coli was studied, thus assessing its response to induced environmental stress factors. Methods and results A kinetic assay has been performed using a microplate reader with a spectrofluorometer to determine the specific growth rates. Polynomial models were developed to correlate the environmental conditions of temperature and carbon dioxide with E. coli BL21 (DE3) growth in culture media and dairy by-products. At a temperature of 42 °C, as the dissolved CO2 increased, a decrease of the μmax by 0.76 h−1 was observed. In contrast, at 27 °C, this increase led to a rise of the μmax by 0.99 h−1. Moreover, a correction factor was added when applying the model to dairy whey samples. Conclusions The application of this developed model can be considered a useful tool for predicting the growth of E. coli using climate projections.

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Section: Discussionmentioning

confidence: 99%

The role of temperature and carbon dioxide climatic stress factors on the growth kinetics ofEscherichia coli

Roufou

Griffin

Attard

et al. 2023

Journal of Applied Microbiology

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“…Bioluminescent microorganisms or lux reporter system can be applicable as biosensors for detection of water quality, lethality testing, for the discovery of anti-toxin deposits and pathogens in sustenance (Nyhan et al . 2020 ; Park et al . 2018 ).…”

Section: Discussionmentioning

confidence: 99%

A quantitative method to assess bacterial adhesion using recombinant bioluminescent <i>Pseudomonas aeruginosa</i>

Wang¹,

Qiao²,

Gao³

et al. 2021

Biophysics Reports

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Bioluminescence technology has been widely used in the field of medical detection. The bioluminescent lux reporter system provides a non-invasive platform to monitor bacterial growth and expression in real time. This study aimed to establish a method for detecting bacterial adhesion on the surface of materials, including medical devices, by using recombinant bioluminescent Pseudomonas aeruginosa containing a lux reporter. By monitoring the growth and bioluminescent properties of the recombinant PAO1- lux strain, the optimal test conditions for bacterial adhesion detection in vitro were determined to be as follows: an initial inoculation density of 10 5 to 10 6 CFU/mL, M9 medium at a pH 6.2, an adhesion time of 6 h, and the collection of adherent bacteria by ultrasonic cleaning. The traditional CFU counting method and the bioluminescence method were compared, and the applicability of the new method was verified by testing the adhesion of bacteria on the surface of various materials. The validated bioluminescent strains could serve as strong candidates to be used as bacterial detection tools in applications such as bacterial adhesion evaluation as well as supplements and alternatives to traditional microbiological testing procedures. In addition, this method has the potential to enable the study of bacterial adhesion on the surface of inanimate objects and living tissues. With the development of this method and its wide applicability, it is expected to become a standard method for the detection of bacterial adhesion and the screening of anti-adhesion materials.

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The Influence of Food Matrices on Microbial Growth

Myintzaw,

Callanan

2023

Methods and Protocols in Food Science

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An evaluation of Lux technology as an alternative methodology to determine growth rates of Listeria in laboratory media and complex food matrices

Cited by 6 publications

References 41 publications

The role of temperature and carbon dioxide climatic stress factors on the growth kinetics ofEscherichia coli

The role of temperature and carbon dioxide climatic stress factors on the growth kinetics ofEscherichia coli

A quantitative method to assess bacterial adhesion using recombinant bioluminescent <i>Pseudomonas aeruginosa</i>

The Influence of Food Matrices on Microbial Growth

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