An evaluation of microbial coronal leakage in the restored pulp chamber of root‐canal treated multirooted teeth

Chailertvanitkul, Pattama; Saunders, W. P.; Saunders, E. M.; MacKenzie, Duncan

doi:10.1046/j.1365-2591.1997.00089.x

Cited by 29 publications

(31 citation statements)

References 20 publications

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“…In this study, the turbidity of the culture medium was used as reference and an indicator of microbial microleakage (a qualitative microbial analyze than quantitative). Chailertvanitkul et al (19) reported that in one qualitative analyze the microleakage is evidenced as positive only when occurred the turbidity on culture medium, indicating complete penetration of microorganisms through the restorative and root canal filling materials. Considering identical explanation, they reported that the number of microorganisms required to cause apical periodontitis until is unknown.…”

Section: Discussionmentioning

confidence: 99%

Microbial leakage of MTA, Portland cement, Sealapex and zinc oxide-eugenol as root-end filling materials

Estrela

Estrada-Bernabé²,

Decúrcio³

et al. 2011

Med Oral

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Objective: The aim of this study was to compare the microbial leakage of mineral trioxide aggregate (MTA), Portland cement (PC), Sealapex and zinc oxide-eugenol (ZOE) as root-end filling materials. Study design: An in vitro microbial leakage test (MLT) with a split chamber was used in this study. A mixture of facultative bacteria and one yeast (S. aureus + E. faecalis + P. aeruginosa + B. subtilis + C. albicans) was placed in the upper chamber and it could only reach the lower chamber containing Brain Heart Infusion broth by way of leakage through the root-end filling. Microbial leakage was observed daily for 60 days. Sixty maxillary anterior human teeth were randomly assigned to different groups -MTA and PC (gray and white), Sealapex + zinc oxide and ZOE, control groups and subgroups to evaluate the influence of EDTA for smear layer removal. These materials were further evaluated by an agar diffusion test (ADT) to verify their antimicrobial efficacy. Data were analyzed statistically by Kruskal-Wallis and Mann-Whitney test. Results: In the MLT, Sealapex + zinc oxide and ZOE did not show evidence of microbial leakage over the 60-day experimental period. The other materials showed leakage from the 15th day. The presence of smear layer influenced microbial leakage. Microbial inhibition zones were not observed in all samples tested by ADT. Conclusion: Sealapex + zinc oxide and ZOE did not show microbial leakage over the experimental period, whereas it was verified within 15 to 45 days in MTA and Portland cement.

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Section: Discussionmentioning

confidence: 99%

Microbial leakage of MTA, Portland cement, Sealapex and zinc oxide-eugenol as root-end filling materials

Estrela

Estrada-Bernabé²,

Decúrcio³

et al. 2011

Med Oral

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“…First, the apical seal may be affected adversely and cause the root canal treatment to fail. Second, movement of microorganisms and toxins through accessory canals in the floor of the pulp chamber may result in periodontal furcation involvement (39).…”

Section: Bacteria and Toxin Infiltration Methodsmentioning

confidence: 99%

“…If only one bacterium passes through the obturated root canal, it may multiply in the enriched broth and cause turbidity (38,39).…”

Section: Bacteria and Toxin Infiltration Methodsmentioning

confidence: 99%

Methodologies for assessment of apical and coronal leakage of endodontic filling materials: a critical review

Veríssimo

Vale

2006

Journal of Oral Science

137

110

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Keywords: dye dilution technique; dyes; filtration. IntroductionMicroorganisms present inside root canals may remain active in the dentinal tubules even after vigorous chemicalmechanical preparation. Thus, perfect apical sealing is desirable to prevent the remaining bacteria and their endotoxins from reaching the root apex (1,2). Apical leakage is considered to be a common cause for endodontic therapy failure, and is influenced by many variables such as different filling techniques, the physical and chemical properties of sealers and the presence or absence of a smear layer (1,3). In coronal leakage, the canal may be recontaminated in various ways such as contact between the oral bacterial flora and root canal tubule inlets. However, it most frequently occurs as a result of loss of temporary filling, or inadequate endodontic filling or crown sealing (4,5).According to Timpawat et al. (6), endodontic sealers are used to eliminate the interface between the gutta-percha and the dentinal walls. Leakage may, however, occur at the interfaces between the sealer and dentin, sealer and gutta-percha and in spaces within the sealer itself. Thus, the quality of the filling depends largely on the sealing capacity offered by sealers (7,8).Methodologies in vitro are used to estimate sealing quality, generally by measuring microleakage that allows the tracer agent to penetrate the filled canal. Commonly used tracers are dyes, radioisotopes, bacteria and their products, such as endotoxins. Bacteria used as tracers most closely approximate what happens clinically in terms of leakage (9-11). Other methodologies, such as fluid filtration and dye extraction methods have also been used, their main advantage being high reproducibility (1,(12)(13)(14).The aim of this review was to critically analyze the various methodologies reported in the literature for assessing root canal sealing capacity. Apparently, they are not standardized, which makes it difficult to compare the results obtained when different methodologies are used, even though the same substrate is being assessed. Literature ReviewAlthough there are many leakage studies, there is no consensus about the endodontic sealer and core material sealing capacities. One of the reasons is that investigations do not use a standardized methodology and this frequently

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“…However, those bacterial studies have been qualitative rather than quantitative. If only one bacterium passes through the obturated root canal, it may multiply in the enriched broth and cause turbidity 37,38) . The use of bacteria to assess coronal leakage also has its limitation because some of sealing materials have antibacterial activities 39) .…”

Section: Discussionmentioning

confidence: 99%

<i>In vitro</i> microleakage of six different dental materials as intraorifice barriers in endodontically treated teeth

et al. 2015

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The aim of this study was to compare the coronal sealing ability of six different dental materials: Three MTA-based cements and three established restorative materials by in vitro dye penetration method. For in vitro infiltration experiments, seventy extracted single-rooted human teeth were used. After crowns of teeth were reduced, root canals were prepared, and filled with gutta-percha cone. Teeth were randomly divided into 6 groups with 10 teeth per group. The orifice of each tooth was prepared to 3 mm depth and filled with the following materials: (I) ProRoot WMTA; (II) EndoCem Zr; (III) Angelus White; (IV) LuxaCore; (V) Fuji II LC; and (VI) Elite. After 5,000 cycles of thermocycling between 5°C and 55°C, dye penetration of each specimen was measured. The order of less dye infiltration of coronal filling materials was: ProRoot WMTA

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An evaluation of microbial coronal leakage in the restored pulp chamber of root‐canal treated multirooted teeth

Cited by 29 publications

References 20 publications

Microbial leakage of MTA, Portland cement, Sealapex and zinc oxide-eugenol as root-end filling materials

Microbial leakage of MTA, Portland cement, Sealapex and zinc oxide-eugenol as root-end filling materials

Methodologies for assessment of apical and coronal leakage of endodontic filling materials: a critical review

<i>In vitro</i> microleakage of six different dental materials as intraorifice barriers in endodontically treated teeth

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