An evaluation of some commercial lead(II)-selective electrodes

Kivalo, P.; Virtanen, Rauno; Wickström, Kim; Wilson, M.; Püngor, E.; Horvai, George; Tóth, Klára

doi:10.1016/s0003-2670(01)82268-6

Cited by 37 publications

(15 citation statements)

References 22 publications

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“…Pb(NO3h solutions and , and a range of free Pb 2+ concentrations down to 10 ~2 M is obtained by varying the pH of the Pb/NTA buffers. The Pb 2+ electrode gives a linear response in the range from 10 2 to 10 -12 M Pb 2+ (Kivalo et al, 1976), and this was confirmed in the present work (not shown).…”

Section: Lead Bufferssupporting

confidence: 89%

“…Samples results are given in Fig. 4(b); the log Ka measured Kivalo et al (1976). in three separate experiments was 7.00, 7.03 and 7.02, mean 7.02.…”

Section: Calibration Of Pb/tiron Buffersmentioning

confidence: 91%

“…The Pb 2+ electrode was calibrated in two ways: either just with unbuffered Pb solutions, or with both unbuffered and buffered solutions. The calibration of the Orion Pb 2+ electrode by the latter method has been described (Kivalo et al, 1976). Pb(NO3h solutions and , and a range of free Pb 2+ concentrations down to 10 ~2 M is obtained by varying the pH of the Pb/NTA buffers.…”

Section: Lead Buffersmentioning

confidence: 99%

“…The former value has been used in the calibration of Pb 2+ electrodes (Kivalo et al, 1976). In the present experiments the effective dissociation constant for Pb-NTA was measured at room temperature (23 to 25~ using the method of Bers (1982).…”

Section: Calibration Of Pb/nta Buffersmentioning

confidence: 99%

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Influence of lead ions on cation permeability in human red cell ghosts

Simons

1985

J. Membrain Biol.

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Intracellular Pb2+ ions can replace Ca2+ ions in stimulating the Ca-dependent K permeability of human red blood cells. In metabolically depleted resealed ghosts, the threshold for stimulation of 86Rb efflux by internal Pb2+ is around 5 X 10(-10) M, and stimulation is half-maximal at about 2 X 10(-9) M, and maximal at 10(-8) M Pb2+. There is no effect on 22Na efflux in this concentration range. 86Rb efflux is antagonized by internal Mg2+ ions, and by the channel-blocking drugs quinidine and diS-C2(5), as observed for the Ca-dependent K permeability in red cells. In ghosts containing EDTA, which prevents any internal effects of Pb2+ ions, external Pb2+ increases both 22Na and 86Rb permeability when its concentration exceeds 6 X 10(-7) M. This effect is seemingly unrelated to the Ca-dependent K permeability. This work makes extensive use of Pb2+ ion buffers, and gives information about their preparation and properties.

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Section: Lead Bufferssupporting

confidence: 89%

“…Samples results are given in Fig. 4(b); the log Ka measured Kivalo et al (1976). in three separate experiments was 7.00, 7.03 and 7.02, mean 7.02.…”

Section: Calibration Of Pb/tiron Buffersmentioning

confidence: 91%

Section: Lead Buffersmentioning

confidence: 99%

Section: Calibration Of Pb/nta Buffersmentioning

confidence: 99%

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Influence of lead ions on cation permeability in human red cell ghosts

Simons

1985

J. Membrain Biol.

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“…For Pb uptake, 1.5 mM Pb(N03)2 and 3 mM (K) citrate were added. This buffer mixture gives a free Pb2+ concentration of 2.6pM, measured with the Orion 94-82A Pb ion-sensitive electrode, calibrated as in [8]. The Pb content of the cells was determined either after centrifuging a portion of suspension through Dow Corning 550 silicone oil in a 1.5 ml plastic centrifuge tube and cutting off the cell pellet (experiment in fig.…”

Section: Methodsmentioning

confidence: 99%

Active transport of lead by human red blood cells

Simons

1984

FEBS Letters

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Human red cells suspended in lead‐citrate buffers (2.6μM Pb2+) take up much less Pb than predicted from studies of equilibrium binding of Pb to haemolysates. Pb uptake is increased by ATP depletion, or by loading at 0°C. Tracer studies with 203Pb indicate that the low uptake at 37°C in the presence of substrate is not due to membrane impermeability to Pb. Cold‐loaded cells extrude Pb against a concentration gradient at 37°C when glucose is present. These results suggest that the cellular loading of Pb is dependent on the balance between an inward leak and an outward pump. The extrusion of Pb from the cells is possibly brought by the Ca pump.

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