An evaluation of the efficacy of compound alpha and triclabendazole against two isolates of Fasciola hepatica

McConville, M.; Brennan, Gerard; Flanagan, Ailish; Edgar, H.W.J.; Hanna, R.E.B.; McCoy, M. A.; Gordon, Alan; Castillo, Rafael; Hernández‐Campos, Alicia; Fairweather, Ian

doi:10.1016/j.vetpar.2009.02.004

Cited by 56 publications

(58 citation statements)

References 43 publications

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“…The spines on the tail surface were smaller than the spines covering the rest of the fluke's surface (Figure 1g and inset). No significant differences were observed between fresh fluke and control fluke incubated for 24 h in solvent; 0.06% (v/v) Cremiphore EL.…”

Section: Scanning Electron Microscopy (Sem) Of Normal Fresh and Contrmentioning

confidence: 85%

“…The flukes were incubated with 10,30 and 60 毺g/mL of Mirazid襅 oleoresin and myrrh volatile oil for 24 h at 37 曟 in an atmosphere of 5% CO 2 . A positive control group was prepared by incubating whole flukes for 24 h in RPMI 1640 culture medium containing 15 毺g/mL TCBZ-SO. This level corresponded to maximum blood levels in vivo [20] .…”

Section: In Vitro Assaysmentioning

confidence: 99%

“…Following incubation, the adult flukes were rinsed with 0.9% NaCl (w/v) and fixed with 2.5% (v/v) glutaraldehyde in a PBS buffer (pH 7.4) for 24 h at room temperature. After rinsing with PBS buffer, the specimens were washed with distilled water, dehydrated in ascending ethanol concentrations and critically point dried in carbon dioxide.…”

Section: Scanning Electron Microscopymentioning

confidence: 99%

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Effects of Mirazid® and myrrh volatile oil on adult Fasciola gigantica under laboratory conditions

Am¹,

Shalaby²,

El-Khateeb³

et al. 2012

Asian Pacific Journal of Tropical Biomedicine

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Section: Scanning Electron Microscopy (Sem) Of Normal Fresh and Contrmentioning

confidence: 85%

Section: In Vitro Assaysmentioning

confidence: 99%

Section: Scanning Electron Microscopymentioning

confidence: 99%

See 1 more Smart Citation

Effects of Mirazid® and myrrh volatile oil on adult Fasciola gigantica under laboratory conditions

Am¹,

Shalaby²,

El-Khateeb³

et al. 2012

Asian Pacific Journal of Tropical Biomedicine

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“…The infectivity of F. gigantica was found to be low in Wistar rats (Itagaki et al 1994). Rabbits, the rat-like hamster, Tscherskia triton, and sheep have also been used for studies of experimental fascioliasis (Terasaki et al 2003 ;McConville et al 2009). …”

Section: F Hepatica In Vitro Assaysmentioning

confidence: 99%

In vitroandin vivotrematode models for chemotherapeutic studies

2009

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Schistosomiasis and food-borne trematodiases are chronic parasitic diseases affecting millions of people mostly in the developing world. Additional drugs should be developed as only few drugs are available for treatment and drug resistance might emerge. In vitro and in vivo whole parasite screens represent essential components of the trematodicidal drug discovery cascade. This review describes the current state-of-the-art of in vitro and in vivo screening systems of the blood fluke Schistosoma mansoni, the liver fluke Fasciola hepatica and the intestinal fluke Echinostoma caproni. Examples of in vitro and in vivo evaluation of compounds for activity are presented. To boost the discovery pipeline for these diseases there is a need to develop validated, robust high-throughput in vitro systems with simple readouts.

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“…It has been characterized as showing resistance to the action of triclabendazole in vivo at both adult and juvenile stages (Coles et al, 2000;Coles & Stafford, 2001;McCoy et al, 2005;McConville et al, 2009a), as well as resistance to the TCBZ metabolite, triclabendazole sulphoxide in vitro (Robinson et al, 2002;Mottier et al, 2006a). Six male Sprague -Dawley rats under isoflurane anaesthesia were each orally infected, by means of a stomach tube, with 20 metacercarial cysts of the Sligo isolate of F. hepatica.…”

Section: Fluke Materialsmentioning

confidence: 99%

Adult triclabendazole-resistantFasciola hepatica: morphological changes in the tegument and gut followingin vivotreatment with artemether in the rat model

O'Neill

Johnston²,

Halferty³

et al. 2009

J. Helminthol.

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A study has been carried out to determine the morphological changes to the adult liver fluke, Fasciola hepatica after treatment in vivo with artemether. Rats were infected with the triclabendazole-resistant Sligo isolate of F. hepatica, dosed orally with artemether at a concentration of 200 mg/kg and flukes recovered at 24, 48 and 72 h post-treatment (p.t.). Surface changes were monitored by scanning electron microscopy and fine structural changes to the tegument and gut by transmission electron microscopy. Twenty-four hours p.t., the external surface showed minor disruption, in the form of mild swelling of the tegument. The tegumental syncytium and sub-tegumental tissues appeared relatively normal. Forty-eight and seventy-two hours p.t., disruption to the tegumental system increased, with isolated patches of surface blebbing and reduced production of secretory bodies by the tegumental cells being the main changes seen. The gastrodermal cells showed a relatively normal morphology 24 h p.t. By 48 h, large numbers of autophagic vacuoles and lipid droplets were present. Autophagy increased in magnitude by 72 h p.t. and substantial disruption to the granular endoplasmic reticulum was observed. Results from this study show that flukes treated in vivo with artemether display progressive and timedependent alterations to the tegument and gut. Disruption to the gut was consistently and substantially more severe than that to the tegument, suggesting that an oral route of uptake for this compound predominates. This is the first study providing ultrastructural information on the effect of an artemisinin compound against liver fluke.

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An evaluation of the efficacy of compound alpha and triclabendazole against two isolates of Fasciola hepatica

Cited by 56 publications

References 43 publications

Effects of Mirazid® and myrrh volatile oil on adult Fasciola gigantica under laboratory conditions

Effects of Mirazid® and myrrh volatile oil on adult Fasciola gigantica under laboratory conditions

In vitroandin vivotrematode models for chemotherapeutic studies

Adult triclabendazole-resistantFasciola hepatica: morphological changes in the tegument and gut followingin vivotreatment with artemether in the rat model

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