An Evaluation of the Frequency of Spontaneous Aneuploidy in Human Somatic Cells Using the Technology of Interphase Cytogenetics

Nazarenko, S. A.; Timoshevsky, V. A.

doi:10.1023/b:ruge.0000016986.78122.5b

Cited by 4 publications

(7 citation statements)

References 38 publications

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“…Thus, the AE frequency in cultivated cells of individuals exposed to adverse environmental factors after one DNA replication cycle was higher than in noncultivated cells by 52% on average ( P = 0.01). Our earlier study of the control group showed that the increase in the total frequency of numerical chromosome aberrations after cultivation of cells from individuals not exposed to domestic or occupational mutagenic factors was as small as 23%: 1.84 ± 0.73% in noncultivated and 2.26 ± 0.93% in cultivated leukocytes ( P = 0.25) [8]. Thus, adverse environmental factors increase the proportion of aneuploid cells by more than twofold, and this effect is observed only in dividing cells (Fig.…”

Section: Resultsmentioning

confidence: 93%

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Comparison of the rates of aneuploidy occurrence in quiescent and dividing cells of humans exposed to adverse environmental factors

Nazarenko¹,

Timoshevsky²

2005

Russ J Genet

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Fluorescence in situ hybridization (FISH) was used to compare aneuploidy rates in four autosomes and two sex chromosomes in interphase nuclei of noncultivated (quiescent) and cultivated (induced to divide with phytohemagglutinin (PHA)) leukocytes in people engaged in nuclear-chemical industry and in a control group of people not exposed to mutagenic factors occupationally or at home. The overall rates of numerical chromosome aberrations for all of the six chromosomes studied showed little difference, although a higher rate of loss of the X-and Y-chromosomes was observed in the exposed group. In individuals exposed to several adverse environmental factors, the overall rate of numerical chromosome aberrations in cultivated cells after at least one DNA replication cycle exceeded that in noncultivated cells by 52% ( P = 0.01), whereas only a trend for its increase was observed in the control group (23%, P = 0.25). Thus, the effect of adverse environmental factors in humans caused more than a twofold increase in the difference between the rates of aneuploid cells in cultivated and noncultivated leukocytes in the exposed group as compared to control. It is conjectured that cell division is accompanied by the expression of potential damage of mitotic chromatid segregation apparatus accumulated in vivo. These defects, realized during cell division, bring about numerical chromosome aberrations.

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Section: Resultsmentioning

confidence: 93%

“…A total of 154 450 cells were examined. Hybridization signals in interphase nuclei were counted according to criteria developed on the basis of our experience in interphase FISH analysis and data from literature [6][7][8][9].…”

Section: Comparison Of the Rates Of Aneuploidy Occurrencementioning

confidence: 99%

Comparison of the rates of aneuploidy occurrence in quiescent and dividing cells of humans exposed to adverse environmental factors

Nazarenko¹,

Timoshevsky²

2005

Russ J Genet

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“…To discover aneuploidy some researchers have used the method of fluorescence in situ hybridization (FISH) of particular chromosomes of the cell nucleus, since with this technology it is possible to analyze ten times as many cells as in standard cytogenetic analysis [68]. By means of this method information can be obtained on the frequency of X chromosome aneuploidy in females for the purpose of diagnosing both the mosaic variant of X-monosomy (Shereshevskii-Turner syndrome) and the mosaic variant of X chromosome trisomy (triplo-X syndrome) along with different variants of X polysemy in the Klinefelter syndrome in males [69].…”

Section: Aneuploidymentioning

confidence: 99%

“…However, the relationship between changes in different characteristics of the karyotype and the evolution of the tumorigenicity of cells has yet to be studied [68]. It is believed that the karyotypic variability may be the basis for the evolution of tumor cells.…”

Section: Aneuploidymentioning

confidence: 99%

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Cytogenetic anomalies and causes for their occurrence in somatic cells

Kovaleva

2008

Cytol. Genet.

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Different types of cytogenetic anomalies used in classical cytogenetics for estimating the level of damage to the chromosome apparatus are considered. Possible causes for the occurrence of different types of cytogenetic anomalies as well as the range of methods of micronucleus testing are discussed. It is shown that different levels of organization of genetic material (nucleotide, chromosome, or suprachromosome material) have an effect on processes involved in realization of a defect in the nucleotide sequence into a cytogenetic anomaly.

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Programmed DNA Elimination in Vertebrates

Smith

Timoshevskiy

Saraceno

2021

Annu. Rev. Anim. Biosci.

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Over the last few decades, an increasing number of vertebrate taxa have been identified that undergo programmed genome rearrangement, or programmed DNA loss, during development. In these organisms, the genome of germ cells is often reproducibly different from the genome of all other cells within the body. Although we clearly have not identified all vertebrate taxa that undergo programmed genome loss, the list of species known to undergo loss now represents ∼10% of vertebrate species, including several basally diverging lineages. Recent studies have shed new light on the targets and mechanisms of DNA loss and their association with canonical modes of DNA silencing. Ultimately, expansion of these studies into a larger collection of taxa will aid in reconstructing patterns of shared/independent ancestry of programmed DNA loss in the vertebrate lineage, as well as more recent evolutionary events that have shaped the structure and content of eliminated DNA. Expected final online publication date for the Annual Review of Animal Biosciences, Volume 9 is February 16, 2021. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

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An Evaluation of the Frequency of Spontaneous Aneuploidy in Human Somatic Cells Using the Technology of Interphase Cytogenetics

Cited by 4 publications

References 38 publications

Comparison of the rates of aneuploidy occurrence in quiescent and dividing cells of humans exposed to adverse environmental factors

Comparison of the rates of aneuploidy occurrence in quiescent and dividing cells of humans exposed to adverse environmental factors

Cytogenetic anomalies and causes for their occurrence in somatic cells

Programmed DNA Elimination in Vertebrates

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