An evaluation of the mouse sperm morphology test and other sperm tests in nonhuman mammals

Wyrobek, Andrew J.; Gordon, Laurie; Burkhart, James G.; Francis, Mary W.; Kapp, Robert W.; Letz, Gideon; Malling, H.V.; Topham, J.C.; Whorton, M D

doi:10.1016/0165-1110(83)90014-3

Cited by 364 publications

(139 citation statements)

References 57 publications

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“…35) Sperm morphologies are qualitative indexes of spermatogenesis. 28) In the present study, DEPs affected these two indexes of spermatogenesis in the same manner as in our previous studies. 14,15) DSP in all DEPtreated groups, except for the 0.3% Q + DEP group, were significantly decreased as compared with that in the vehicle group.…”

Section: Discussionsupporting

confidence: 86%

“…10,26) Hence, the spermatid count was divided by 4.84 to obtain the DSP as well as reports which were written about reproductive toxicity-induced DE. 10,11,13,27) Sperm morphology was evaluated by the procedures of Wyrobek et al 28) and Watanabe et al, 29) with minor modifications. Sperm smears were created using a suspension collected immediately from the left cauda epididymis into 4 ml of physiological saline at 37 C. The sperm preparations were stained with 1% eosin Y solution and examined under a light microscope.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Alleviative Effects of Quercetin and Onion on Male Reproductive Toxicity Induced by Diesel Exhaust Particles

Izawa

Kohara

Aizawa³

et al. 2008

Bioscience, Biotechnology, and Biochemistry

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Section: Discussionsupporting

confidence: 86%

Section: Methodsmentioning

confidence: 99%

Alleviative Effects of Quercetin and Onion on Male Reproductive Toxicity Induced by Diesel Exhaust Particles

Izawa

Kohara

Aizawa³

et al. 2008

Bioscience, Biotechnology, and Biochemistry

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“…Their heart, lungs, kidneys, liver and reproductive organs (testicles and epididymis) were removed and weighed and it was also developed the morphologic evaluation of spermatozoids contained in the epididymis, according methodology described by Wyrobek et al (1983). The blades were prepared and stained with Shorr and 200 cells per animal were analyzed in optical microscopy using an amplification of x1000.…”

Section: Methodsmentioning

confidence: 99%

Toxic effects of different doses of cyclophosphamide on the reproductive parameters of male mice

Kanno

Sensiate

Paula

et al. 2009

Braz. J. Pharm. Sci.

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The cyclophosphamide is used in cancer treatment. The aim of this study was evaluating the effect of different doses of this drug on male mice reproductive parameters. The cyclophosphamide was administered in the doses 100, 150, 200 e 250 mg.kg -1 , intraperitoneal route, for six weeks. As a result, it was observed a decrease in body mass and a decrease in testicles and kidney's weight, in all animals treated with cyclophosphamide. Only the groups that received the doses 100, 150 mg.kg -1 of cyclophosphamide were able to fertilize their females. There was higher incidence of post-implantation losses, reabsorptions and decrease in fetal viability in the group that received the dose of 150 mg.kg . Based on the methodology used and results obtained, it was concluded that the cyclophosphamide was toxic, considering the decrease in animal's body mass and testicle's weight; promoted hepatotoxicity and nephrotoxic effect; influenced in the animals spermatogenesis taking them to infertility and/or subfertility; decreased fetal viability, despite it didn't cause significant malformations in the offspring.Uniterms: Cyclophosphamide/effects. Cyclophosphamide/toxicity. Spermatogenesis/experimental study. Chemotherapeutics/adverse effects. Fertility/experimental study.A ciclofosfamida é utilizada no tratamento de câncer. Este estudo visa avaliar os efeitos das diferentes doses do fármaco nos parâmetros reprodutivos de camundongos machos. A ciclofosfamida foi administrada nas doses de 100, 150, 200 e 250 mg kg -1 , via intraperitoneal por seis semanas. Como resultado observou-se diminuição de massa corporal, redução no peso de testículos e rins em todos os animais tratados com a ciclofosfamida. Apenas os grupos que receberam as doses de 100 e 150 mg kg -1 do quimioterápico foram capazes de fertilizar as fêmeas. Houve maior incidência de perdas pós-implantação, reabsorção e diminuição da viabilidade fetal no grupo que recebeu a dose de 150 mg kg -1 . Observou-se redução nos pesos dos epidídimos e fígado dos animais tratados com as doses de 150, 200 e 250 mg kg -1 . Espermatozóides anômalos foram encontrados nas doses de 200 e 250mg kg -1 . Com base na metodologia empregada e nos resultados obtidos, conclui-se que a ciclofosfamida foi tóxica considerando-se a redução de massa corporal e o peso dos testículos dos animais; promoveu hepatotoxicidade e efeito nefrotóxico; influenciou na espermatogênese dos animais de forma a levá-los a um estado de infertilidade e/ou subfertilidade; diminuiu viabilidade fetal, entretanto não causou malformações significativas na prole.Unitermos: Ciclofosfamida/efeitos. Ciclofosfamida/toxicidade. Espermatogênese/estudo experimental. Quimioterápicos/efeitos adversos. Fertilidade/ estudo experimental.

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“…A large number of physical and chemical factors are known to temporarily increase the inci¬ dence of malformed spermatozoa Wyrobek et al, 1983;Pogany, 1987). Although means of selecting against these deformed spermatozoa within the female reproductive tract have been demonstrated (Krzanowska, 1974), embryos conceived during the time that the frequency of sperm shape abnormality is exacerbated may be at considerable genetic and/or teratogenic risks.…”

Section: Introductionmentioning

confidence: 99%

Quantitative fluorometry of abnormal mouse sperm nuclei

Pogany¹,

Balhorn²

1992

Reproduction

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Summary. An extensive quantitative analysis of deformed mouse spermatozoa was undertaken. Improvements over previous studies included the isolation and purification of sperm nuclei, a multifaceted analytical approach using several fluorochromes and the analysis of individual nuclei classified into shape categories.Malformed sperm nuclei in BALB/c mice could not be distinguished from normal ones in terms of total and basic proteins, sulfhydryl and disulfide group concentration, DNA concentration and chromatin organization. The shape of sperm nuclei is therefore probably determined by the manner in which the internal biochemical components are assembled.

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An evaluation of the mouse sperm morphology test and other sperm tests in nonhuman mammals

Cited by 364 publications

References 57 publications

Alleviative Effects of Quercetin and Onion on Male Reproductive Toxicity Induced by Diesel Exhaust Particles

Alleviative Effects of Quercetin and Onion on Male Reproductive Toxicity Induced by Diesel Exhaust Particles

Toxic effects of different doses of cyclophosphamide on the reproductive parameters of male mice

Quantitative fluorometry of abnormal mouse sperm nuclei

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