An evidence of Humanin-like peptide and Humanin mediated cryosurvival of spermatozoa in buffalo bulls

Katiyar, Rahul; Ghosh, Subrata; Karikalan, M.; Kumar, Abhishek; Pande, Megha; Gemeda, Amare Ishetu; Rautela, Rupali; Dhara, Sujoy K.; Bhure, S K; Srivastava, N.; Patra, Manas Kumar; Chandra, Vikash; Devi, Huidrom Lakshmi; Singh, Mastan

doi:10.1016/j.theriogenology.2022.09.013

Cited by 5 publications

(5 citation statements)

References 71 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We expected to see positive direct effects of antioxidants on motility but did not. Thus, removing antioxidants with lower loadings like melatonin and NGF may be advantageous, with the possibility of substituting more powerful antioxidants like proline [ 31 ] and humanin [ 1 ].…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Optimizing Bull Semen Cryopreservation Media Using Multivariate Statistics Approaches

Mokhtassi‐Bidgoli

Sharafi²,

Benson

2023

Animals

View full text Add to dashboard Cite

Cryo-injury reduces post-thaw semen quality. Extender components play a protective role, but existing experimental approaches do not elucidate interactions among extender components, semen samples, and post-thaw quality. To identify optimal concentrations for 12 extender ingredients, we ran 122 experiments with an I-optimal completely random design using a large dataset from our previous study. We obtained a maximum predicted total motility of 70.56% from an I-optimal design and 73.75% from a Monte Carlo simulation. Individual bull variations were significant and interacted with extenders independently. 67% of bulls reliably preferred extender formulations to reach maximum motility. Multifactor analysis suggests that some antioxidants may offer superior protection over others. Partial least squares path modeling (PLS-PM) found the highest positive loadings for glutathione in the antioxidant class, glycerol in the CPA class, and fructose in the basic compounds class. The optimal ranges for milk, water, and ethylene glycol were extremely narrow. Egg yolk, cholesterol-loaded cyclodextrin, and nerve growth factor had medium-loading impacts. PLS-PM showed that CPA, osmoregulators, and basic components were the most efficient contributors to motility, while the antioxidant and extracellular protectant classes had less efficiency. Thus, ingredients, concentrations, and interactions of extender compounds are critical to extender formulation, especially when using multiple compounds with the same function.

show abstract

Section: Resultsmentioning

confidence: 99%

“…Artificial insemination using frozen-thawed sperm is a critical technology in the dairy cattle industry [ 1 , 2 ]. Cryopreserved semen also has a variety of applications for assisted reproductive technology as well as endangered animal preservation [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Optimizing Bull Semen Cryopreservation Media Using Multivariate Statistics Approaches

Mokhtassi‐Bidgoli

Sharafi²,

Benson

2023

Animals

View full text Add to dashboard Cite

show abstract

“…Some studies have evidenced that sperm cells exposed to cold shock, such as via the cryopreservation procedure, indeed exhibited numerous modifications in their structure after examination through TEM, such as acrosome damage, increased plasma membrane fluidity, and mitochondria dysfunction [ 5 , 57 , 58 , 59 ]. In addition, these changes in sperm morphology may induce apoptosis and necrosis-like fluctuations during the cryo-storage and freeze–thaw procedure [ 58 , 59 ].…”

Section: Discussionmentioning

confidence: 99%

Supplementation of Thymoquinone Nanoparticles to Semen Extender Boosts Cryotolerance and Fertilizing Ability of Buffalo Bull Spermatozoa

Khalil,

Hassan,

El-Harairy

et al. 2023

Animals

View full text Add to dashboard Cite

Thymoquinone nanoparticles (TQNPs) are broadly utilized in numerous pharmaceutical applications. In the present study, we tested the effects of TQNP supplementation on sperm quality and kinematics, acrosome exocytosis, oxidative biomarkers, apoptosis-like and morphological changes of frozen–thawed buffalo sperm, as well as the fertilizing capacity. Semen was collected from buffalo bulls, diluted (1:10; semen/extender), and divided into five aliquots comprising various concentrations of TQNP 0 (CON), 12.5 (TQNP12.5), 25 (TQNP25), 37.5 (TQNP37.5), and 50 (TQNP50) µg/mL, and then cryopreserved and stored in liquid nitrogen (−196 °C). The results revealed that TQNPs (25 to 50 µg/mL) provided the most optimal results in terms of membrane integrity (p < 0.001) and progressive motility (p < 0.01). In contrast, TQNP50 resulted in a greater post-thawed sperm viability (p = 0.02) compared with other groups. The addition of TQNPs to the extender had no discernible effects on sperm morphology measures. Sperm kinematic motion was significantly improved in the TQNP50 group compared to the control group (p < 0.01). TQNPs effectively reduced the content of H2O2 and MDA levels and improved the total antioxidant capacity of post-thawed extended semen (p < 0.01). The addition of TQNP significantly increased the number of intact acrosomes (p < 0.0001) and decreased the number of exocytosed acrosomes (p < 0.0001). A significant reduction in apoptosis-like changes was observed in TQNP groups. The non-return rates of buffalo cows inseminated with TQNP50-treated spermatozoa were higher than those in the control group (p < 0.05; 88% vs. 72%). These findings suggested that the freezing extender supplemented with TQNPs could effectively enhance the cryotolerance and fertility of buffalo sperm.

show abstract

“…However, this E. coli -derived enzyme has not been tested in sexed sorted semen. Humanin, on the other hand, protects sperm against oxidative stress and apoptosis ( 79 ). Supplementing Humanin in buffalo's sperm cells has been found to improve freezability despite buffalo sperm sensitivity to cryopreservation ( 79 ).…”

Section: Limitations Associated With Sexed Semenmentioning

confidence: 99%

“…Humanin, on the other hand, protects sperm against oxidative stress and apoptosis ( 79 ). Supplementing Humanin in buffalo's sperm cells has been found to improve freezability despite buffalo sperm sensitivity to cryopreservation ( 79 ). Therefore, these enzymes have the potential to improve sex-sorted sperm quality; however, further studies need to be conducted.…”

Section: Limitations Associated With Sexed Semenmentioning

confidence: 99%

A systematic review on the prospects of X- and Y-sexed semen in ruminant livestock: implications for conservation, a South African perspective

Ngcobo,

Nedambale,

Sithole

et al. 2024

Front. Vet. Sci.

View full text Add to dashboard Cite

South Africa is home to numerous indigenous and locally developed sheep (Nguni Pedi, Zulu, and Namaqua Afrikaner, Afrino, Africander, Bezuidenhout Africander, Damara, Dorper, Döhne Merino, Meat Master, South African Merino, South African Mutton Merino, Van Rooy, and Dorper), goat (SA veld, Tankwa, Imbuzi, Bantu, Boer, and Savanna) and cattle (Afrigus, Afrikaner, Bolowana, Bonsmara, Bovelder, Drakensberger, South African Angus, South African Dairy Swiss, South African Friesland, South African Red, and Veld Master) animals. These breeds require less veterinary service, feed, management efforts, provide income to rural and or poor owners. However, most of them are under extinction risks and some with unknown status hence, require immediate conservation intervention. To allow faster genetic progress on the endangered animals, it is important to generate productive animals while reducing wastages and this can be achieved through sex-sorted semen. Therefore, this systematic review is aimed to evaluate the prospects of X and Y-sexed semen in ruminant livestock and some solutions that can be used to address poor sex-sorted semen and its fertility. This review was incorporated through gathering and assessing relevant articles and through the data from the DAD-IS database. The keywords that were used to search articles online were pre-gender selection, indigenous ecotypes, fertility, flow cytometry, artificial insemination, conservation, and improving sexed semen. Following a careful review of all articles, PRISMA guidelines were used to find the articles that are suitable to address the aim of this review. Sex-sorted semen is a recently introduced technology gaining more attention from researchers particularly, in the conservation programs. Preselection of semen based on the sex chromosomes (X- and or Y-bearing chromosomes) is of paramount importance to obtain desired sex of the offspring and avoid animal wastage as much as possible. However, diverse factors can affect quality of semen of different animal species especially after sex-sorting. Flow cytometry is a common method used to select male and female sperm cells and discard dead and abnormal sperm cells during the process. Thus, sperm sexing is a good advanced reproductive technology (ART) however, it is associated with the production of oxidative stress (OS) and DNA fragmentation (SDF). These findings, therefore, necessitates more innovation studies to come up with a sexing technology that will protect sperm cell injuries during sorting in frozen-thawed.

show abstract

An evidence of Humanin-like peptide and Humanin mediated cryosurvival of spermatozoa in buffalo bulls

Cited by 5 publications

References 71 publications

Optimizing Bull Semen Cryopreservation Media Using Multivariate Statistics Approaches

Optimizing Bull Semen Cryopreservation Media Using Multivariate Statistics Approaches

Supplementation of Thymoquinone Nanoparticles to Semen Extender Boosts Cryotolerance and Fertilizing Ability of Buffalo Bull Spermatozoa

A systematic review on the prospects of X- and Y-sexed semen in ruminant livestock: implications for conservation, a South African perspective

Contact Info

Product

Resources

About