An evolutionary and functional analysis of FoxL2 in rainbow trout gonad differentiation

Báron, Daniel; Cocquet, Julie; Xia, Xuhua; Fellous, Marc; Guiguen, Yann; Veitia, Reiner A.

doi:10.1677/jme.1.01566

Cited by 185 publications

(136 citation statements)

References 29 publications

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“…In trout, a link between estrogens and foxl2a (trout Foxl2 ortholog), based on the temporal gonadal co-expression of foxl2a and cyp19a1, has been postulated (Baron et al, 2004). This hypothesis is strengthened by the early temporal coexpression of cyp19a1 and foxl2a that we found in the present work on gonads of rainbow trout, and by the fact that in the medaka, foxl2 is localized in all the somatic cells also expressing cyp19a1 (Nakamoto et al, 2006).…”

Section: Figsupporting

confidence: 63%

Characterization of early molecular sex differentiation in rainbow trout, Oncorhynchus mykiss

Vizziano

Randuineau²,

Báron³

et al. 2007

Developmental Dynamics

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Early differentiation in rainbow trout gonads was investigated by expression profiling and in situ hybridization (ISH). Expression of cyp19a1 and fst in females and sox9a1 in males were sexually dimorphic between 32 to 35 days post-fertilization (dpf). After 35 dpf, the differentiation proceeded with sexually dimorphic profiles for sox9a2, dmrt1, cyp11b2.1, amh in males and foxl2a, foxl2b, hsd3b1, inha in females. cyp17a1, cyp11a1, star, nr5a1b increased only after 40 dpf in both sexes with a slightly higher expression in females. cyp19a1 expression was localized in a cluster of somatic cells in the ventral side of female gonads, and sox9a2 and amh in somatic cells surrounding the germ cells, at 28 dpf and thereafter, both in male and female gonads. cyp11b2.1, cyp17a1, and cyp11a1 expressions were only detected in scattered somatic cells in males after 46 dpf. This confirms the early implication of cyp19a1 in trout ovarian differentiation and suggests that early testicular differentiation does not need androgen production. Developmental Dynamics

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Section: Figsupporting

confidence: 63%

Characterization of early molecular sex differentiation in rainbow trout, Oncorhynchus mykiss

Vizziano

Randuineau²,

Báron³

et al. 2007

Developmental Dynamics

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171

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“…On the contrary, FOXL2 could not be detected neither in the oocyte, nor in the fetal or adult testes. Identical results have been observed in the mouse, goat, chicken and fish [9,10].…”

Section: Genetics Of Bpes In Humanssupporting

confidence: 74%

Foxl2 gene and the development of the ovary: a story about goat, mouse, fish and woman

et al. 2005

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-In this review, we describe recent results concerning the genetics of sex determination in mammals. Particularly, we developed the study of the FOXL2 gene and its implication in genetic anomalies in goats (PIS mutation) and humans (BPES). We present the expression of FOXL2 in the ovaries of different species. Article published by EDP Sciences and available at http://www.edpsciences.org/rnd or http://dx

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“…They are able to induce male-to-female sex reversal in fish, reptiles, and birds, while counteracting their production (by using, for instance, aromatase inhibitors) leads to female-to-male reversal ( [37] and references therein). The latter fact might now be explained, at least in part, by a defect in the synergy between FOXL2 and ER to control and maintain granulosa cell identity.…”

Section: Pr Ospects and Overviews R A Veitiamentioning

confidence: 99%

“…Although published data are compatible with this idea, it remains to be experimentally shown. [37] Stability and plasticity of a differentiated state…”

Section: Pr Ospects and Overviews R A Veitiamentioning

confidence: 99%

FOXL2 versus SOX9: A lifelong “battle of the sexes”

Veitia

2010

BioEssays

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Testis determination in most mammals is regulated by a genetic hierarchy initiated by the SRY gene. Early ovarian development has long been thought of as a default pathway switched on passively by the absence of SRY. Recent studies challenge this view and show that the ovary constantly represses male-specific genes, from embryonic stages to adulthood. Notably, the absence of the crucial ovarian transcription factor FOXL2 (alone or in combination with other factors) induces a derepression of male-specific genes during development, postnatally and, even more interestingly, during adulthood. Strikingly, in the adult, targeted ablation of Foxl2 leads to a molecular transdifferentiation of the supporting cells of the ovary, which acquire cytological and transcriptomic characteristics of the supporting cells of the testes. These studies bring many answers to the field of gonadal determination, differentiation and maintenance, but also open many questions. Keywords:.F OXL2; ovary determination; ovarian maintenance IntroductionGonadal sex determination is a process that implies a unique decision to make a testis or an ovary out of a bipotential primordium. In most mammals, sex determination is equated with testis determination, whereas ovarian determination has been considered a default process. That is, absence of the testisdetermining factor would automatically lead to the development of an ovary (in appropriate conditions). Recent articles challenge this view and show that in the ovary active mechanisms are required to maintain the differentiated state. [1,2] The bipotential gonad is made up of four presumptive cell lineages: germ cells (which have an extraembryonic origin) and three types of somatic cells. The somatic component involves the supporting cell precursors, which will give rise to Sertoli cells in the male or granulosa cells in the female, the steroidogenic precursors, which differentiate into Leydig cells (male) and theca cells (female), and finally the connective tissue cells yielding other important structures. [3] From a genetic perspective, the SRY gene is pivotal in switching on the testis-determining cascade.[4] Indeed, murine Sry transcripts appear in the presumptive Sertoli cells from 10.5 days postcoitum and for about 2 days, at the right moment for testis determination. [5] In other mammals, including man, SRY is expressed from the period of testis determination until adulthood [6]). It seems now that the main task of Sry/SRY is to directly activate the gene Sox9, which also encodes a transcription factor. Recently a gonad-specific enhancer that mediates testis Sox9 expression, called TESCO, has been identified. [7] Indeed, Sry along with the steroidogenic factor 1 (Sf1) binds to multiple elements within the TESCO. Moreover, mutation, co-transfection, and sex-reversal studies suggest the existence of a positive feedback circuit. First, Sf1 and Sry cooperatively up-regulate Sox9 transcription. Then, Sox9 and Sf1 recognize the enhancer to maintain the expression of the former in the absence of Sr...

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An evolutionary and functional analysis of FoxL2 in rainbow trout gonad differentiation

Cited by 185 publications

References 29 publications

Characterization of early molecular sex differentiation in rainbow trout, Oncorhynchus mykiss

Characterization of early molecular sex differentiation in rainbow trout, Oncorhynchus mykiss

Foxl2 gene and the development of the ovary: a story about goat, mouse, fish and woman

FOXL2 versus SOX9: A lifelong “battle of the sexes”

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