An ex vivo evaluation of antimicrobial efficacy of root canal preparation with hand K-files, hand Pro Taper, and Pro Taper rotary files

Abstract

Background: Microorganisms are considered to be the primary cause behind persistent pulpoperiapical pathologies as well as endodontic failures. It has been found that Enterococcus faecalis is one of the most commonly isolated organisms in failed endodontic treatment. Proper and appropriate mechanical instrumentation is necessary to eradicate these microorganisms. Aims and Objectives: The aim of this study was to compare antimicrobial efficacy of various root canal instrumentation techniques. This study was designed to compare antimicrobial efficacy of root canal preparation using hand K-files, hand Pro Taper files, and Pro Taper rotary files. Materials and Methods: Forty intact maxillary premolars extracted for orthodontic reasons were used. After extirpation of pulp, teeth are decoronated and autoclaved. Then the root canals were infected with E. faecalis suspension and the teeth were incubated at 37°C for 24 h. Thereafter the teeth were divided into five groups and were prepared using the following instruments: group 1 (hand K-files), group 2 (hand Pro Taper files), group 3 (Pro Taper rotary files), group 4 (Control I; treatment consists of irrigation with 1 mL of sterile physiological saline with no instrumentation), and group 5 (Control II; teeth received no treatment at all). Before and after treatments, samples were taken from the root canals for culture and were transferred to physiological saline. Results were evaluated after 24-h incubation in selective culture medium. Results: The results showed that the teeth prepared with Pro Taper rotary files showed significant reduction in number of bacteria from the root canals.

Rotary systems in root canal treatment of primary teeth

“…The use of rotary files demonstrates better canal cleaning compared to manual files in primary molars [38] . Rotary systems have also been shown to have a significant reduction in the number of bacteria in root canals [39] . Both manual and rotary techniques show similar success rates, however, the use of rotary in primary teeth leads to better canal shaping providing better treatment quality in less time [1,21,40,41] .…”

Efficacy of different endodontic procedure with post-operative pain and Quality of Life (QoL) using (OHIP-14).

“…9 Hand NiTi instruments gives better hand control to both amateur and specialist as the cutting and forming velocity can be controlled by the user and this gives more certainty in canal preparation. 10 Hand Pro-Taper NiTi instruments have multiple and dynamic decreases (2-19%), triangular-convex cross area with sharp cutting edges 11 , and a variable helical edge with non-cutting tip. 12 Root canal instruments of this cross-sectional design claim to cut dentine more effectively.…”