An examination of an Antarctic soil metagenomic-derivate putative methylthioadenosine phosphorylase gene as a novel reporter gene for promoter trapping

Abstract

The reporter genes are widely used in promotertrapping vectors for easy and rapid identifi cation of the putative promoter sequences in cloned DNA fragments and in some cases to measure the promoter strength.

Amplicon-Metagenomic Analysis of Fungi from Antarctic Terrestrial Habitats

“…Sequences mainly belonging to Proteobacteria and Actinobacteria were found in metagenomic analysis of soils from the Mars Oasis on Alexander Island ( Pearce et al, 2012 ), and sequences close to the Flavobacterium genus were predominant in surface snow close to Russian stations in Eastern Antarctica ( Lopatina et al, 2016 ). Furthermore, new genes from Antarctica have been found through metagenomic and functional analysis, such as a new cytosolic esterase from the α/β hydrolase family 6 ( Berlemont et al, 2013 ); a methylthioadenosine phosphorylase gene, which was proposed as a novel reporter gene for promoter trapping ( Bartasun et al, 2012 ); bacterial hydrocarbon-degrading genes ( Luz et al, 2010 ); genes encoding proteins that functionally respond to environmental stress such as exopolysaccharides, cold shock proteins, and membrane modifications ( Varin et al, 2012 ); and genes encoding enzymes with potential in industrial applications such as lipase/esterase, amylase, protease and cellulase ( Berlemont et al, 2011 , 2013 ).…”