: This work describes a comparative study of staged and non-staged anaerobic Ðlters for treating a synthetic dairy waste under similar operating conditions. The e †ect of increasing the substrate concentration from 3 to 12 g COD dm~3 at a constant hydraulic residence time (HRT) of 2 days was evaluated with respect to overall reactor performance, biogas production, volatile fatty acids proÐles along the height, methanogenic and acidogenic activity distribution, and hydrodynamic behaviour. The potential maximum speciÐc methanogenic activity against acetate, hydrogen, propionate and butyrate and the lactose speciÐc activity were determined for sludge sampled from three di †erent points in each reactor, under two operating conditions (inÑuent COD of 3 and 9 g COD dm~3). Although all trophic groups involved in the anaerobic process were found throughout the reactors, it was possible to identify di †erent speciÐc sludges at di †erent heights in both reactors. Performances of the two conÐgu-rations were very similar under the operating conditions tested and the plug Ñow behaviour of the staged reactor was clearly reduced when the inÑuent concentration increased from 3 to 9 g COD dm~3.1998 Society of Chemical Industry ( J. Chem. T echnol. Biotechnol. 73, 99È108 (1998)