An Excimer Clamp for Measuring Damaged‐Base Excision by the DNA Repair Enzyme NTH1

Abstract

Supporting information and the ORCID identification number(s) for the author(s) of this article can be found under: https://doi.

Cy5 Dye Cassettes Exhibit Through-Bond Energy Transfer and Enable Ratiometric Fluorescence Sensing

“…Deep-red cyanine dyes are known to self-aggregate in aqueous solution and form nonfluorescent aggregates. On the other hand, pyrene dyes can form excimers in self-aggregation conditions, − ,− and TPE is also well-known for aggregation induced enhancement (AIE). , To probe the change in fluorescent properties upon dye cassette self-aggregation, we acquired absorption (Figure a–d) and emission (Figures S2–S5) spectra for each dye in water/methanol mixtures. In the case of the relatively hydrophobic dyes, 1P or 1T , the transition from 100% methanol to 99.5% water induced minor spectral changes indicating little formation of dye self-aggregates.…”

“…The propensity of pyrene to form excimers with visible emission is often exploited for fluorescence sensing. − For example, the pyrene-based probe L is known to form an excimer complex ( L 2 ·Cu 2+ ) in the presence of Cu 2+ with “turn-on” fluorescence at 430 nm (Figure a) . By simply mixing “responsive” probe L with non-responsive cassette 2P , we achieved ratiometric sensing of Cu 2+ with a single excitation wavelength of 380 nm.…”

“…The past two decades have seen major and ongoing development of intensiometric fluorescent sensors that respond to the presence of specific analytes with “turn on” fluorescence. − Many of the classic examples of fluorescence sensing involve polyaromatic hydrocarbon fluorophores such as pyrene, − tetraphenylethylene (TPE), , and polyaryl­(ethynylene), , and in each case the fluorophore is excited with ultraviolet light (<400 nm), with subsequent emission in the visible region of 400–500 nm. It is well established that many quantitative sensing procedures are more reliable if they monitor a change in the ratio of two emission signals (ratiometric sensing), , and thus there is a need for general methods that convert the classic “turn-on” hydrocarbon fluorescent sensors into ratiometric sensing paradigms.…”

Friedel–Crafts reactions for biomolecular chemistry

“…One of the applications of pyrene nucleotides is the detection of DNA damage (Scheme 5E). 81 The pyrene groups are known to display fluorescence color changes and increase upon a noncovalent dimerization process known as excimer formation, 82 and the design by Kool and co-workers took advantage of the excimer formation when DNA undergoes deletion of a specific nucleotide. 81 In addition to pyrene as a fluorescence reporter, thienoguanosine with a sulfur atom in the ring structure is also a fluorescent analogue with Watson-Crick base pairing ability (Scheme 5F).…”

Synthesis of a fluorescent probe for rhodamine B and determination of Zn2+ content in pharmaceutical zinc gluconate

“…Yang's [14] group designed and synthesized a simple single-and two-photon probe HL to detect Zn 2+ .The probe was highly selective for Zn 2+ and at 500 nm, the fluorescence intensity was significantly enhanced, suggesting a possible reason for this [15] . This paper designed and synthesized a new structure of rhodamine fluorescent probe Y2ST with rhodamine as the parent.…”