An experimental method to facilitate the identification of hybrid sporophytes in the moss <i>Physcomitrella patens</i> using fluorescent tagged lines

Perroud, Pierre‐François; Cove, David J.; Quatrano, Ralph S.; McDaniel, Stuart F.

doi:10.1111/j.1469-8137.2011.03668.x

Cited by 64 publications

(114 citation statements)

References 27 publications

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“…However, these inseminations were never successful in any of the experiments, including those using crosses with wildtype Gd plants. This is in agreement with the previous observations (Perroud et al, 2011) and is likely due the strong potency of the Vx accession as male parent compared to the Gd accession. Therefore, male sterility as a reason for the lack of sporophytes on DPpXLG or DPpGb2 gametophores, although highly likely, can neither be confirmed nor excluded at this point.…”

Section: Inability To Form Sporophyte Is Not Related To Female Sterilitysupporting

confidence: 93%

“…6B). Spores from wild-type Vx 3 Gd crosses germinated with relatively higher frequencies, but also showed a wide range of germination efficiency common for outcrosses between Vx and Gd accessions, as reported previously (Perroud et al, 2011).…”

Section: Spores Lacking Ppxlg or Ppgb2 Are Normalsupporting

confidence: 84%

“…To evaluate whether the sporophyte deficient phenotype is due to the sterility of reproductive organs, we performed crossing experiments using DPpXLG or DPpGb2 with a red fluorescent protein (dsRED) tagged line of P. patens accession Villersexel (Vx) described previously (Perroud et al, 2011). Both DPpXLG and DPpGb2 were able to form sporophytes when grown together with Vx:dsRED in sporulation assays ( Fig.…”

Section: Inability To Form Sporophyte Is Not Related To Female Sterilitymentioning

confidence: 99%

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Sporophyte formation and life cycle completion in moss requires heterotrimeric G-proteins

et al. 2016

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Section: Inability To Form Sporophyte Is Not Related To Female Sterilitysupporting

confidence: 93%

Section: Spores Lacking Ppxlg or Ppgb2 Are Normalsupporting

confidence: 84%

Section: Inability To Form Sporophyte Is Not Related To Female Sterilitymentioning

confidence: 99%

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Sporophyte formation and life cycle completion in moss requires heterotrimeric G-proteins

et al. 2016

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“…The life stage that is most likely to suffer from inbreeding depression is the diploid sporophytic phase . The studies that have attempted to address the relationship between heterozygosity and fitness in sporophytes of various bryophyte species have rendered conflicting results, suggesting that inbreeding depression may be present under certain circumstances but not others Szövényi et al, 2009;Perroud et al, 2011;Jesson et al, 2012;McDaniel and Perroud, 2012;Johnson and Shaw 2015). If the level and risk of inbreeding depression varies greatly between different populations, the selection pressure to avoid inbreeding is also expected to differ.…”

Section: Inbreedingmentioning

confidence: 99%

Balance between inbreeding and outcrossing in a nannandrous species, the moss Homalothecium lutescens

2015

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Epiphytic dwarf males on the females present a possible solution to the problem of short fertilization distances in mosses. However, leptokurtic spore dispersal makes dwarf males likely to be closely related to their host shoot, with an accompanying risk of inbreeding. The capacity of a female to harbour a high number of different dwarf males suggests that there may be mechanisms in place that counteract inbreeding, such as polyandry and post-fertilization selection. We have genotyped sporophytes, female host shoots and dwarf males in four populations of the moss Homalothecium lutescens. We found no evidence of selective sporophyte abortion based on level of heterozygosity. The occurrence of entirely homozygous sporophytes together with significantly positive inbreeding coefficients in three of the populations (mean F IS between 0.48 and 0.64) suggest frequent mother-son mating events. However, 23% of all sampled sporophytes had a higher level of heterozygosity compared with the mean expected heterozygosity at the population level. Polyandry was frequent, on average 59% of the sporophytes on a female shoot were sired by distinct fathers. In conclusion, sporadic fertilizations by dwarf males originating from nonhost female shoots appear to counteract strong inbreeding.

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“…The 100 bud-containing filaments were randomly chosen from different areas of the plate. Tissue for sporophyte production was grown on sterile Jiffy7 soil blocks placed in glass jars under short-day conditions (8 h of light [70-80 mmol m 22 s 21 ]/16 h of dark) at 15°C and manipulated as described (Perroud et al, 2011). Tissue for real-time quantitative RT-PCR was grown and harvested as follows.…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

The DEK1 calpain Linker functions in three-dimensional body patterning in Physcomitrella patens

Johansen¹,

Ako²,

Demko

et al. 2016

Plant Physiol.

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The DEFECTIVE KERNEL1 (DEK1) calpain is a conserved 240-kD key regulator of three-dimensional body patterning in land plants acting via mitotic cell plane positioning. The activity of the cytosolic C-terminal calpain protease is regulated by the membrane-anchored DEK1 MEM, which is connected to the calpain via the 600-amino acid residue Linker. Similar to the calpain and MEM domains, the Linker is highly conserved in the land plant lineage, the similarity dropping sharply compared with orthologous charophyte sequences. Using site-directed mutagenesis, we studied the effect on Physcomitrella patens development by deleting the Linker and two conserved Linker motifs. The results show that removal of the Linker has nearly the same effect as removal of the entire DEK1 gene. In contrast, deletion of the conserved Laminin_G3 (LG3) domain had a milder effect, perturbing leafy gametophore patterning and archegonia development. The LG3 domain from Marchantia polymorpha is fully functional in P. patens, whereas angiosperm sequences are not functional. Deletion of a C-terminal Linker subsegment containing a potential calpain autolytic site severely disturbs gametophore development. Finally, changing one of the three calpain activesite amino acid residues results in the same phenotype as deleting the entire DEK1 gene. Based on the conserved nature of animal and DEK1 calpains, we propose that the DEK1 MEM-Linker complex inactivates the calpain by forcing apart the two calpain subunits carrying the three amino acids of the active site.The three-dimensional (3D) architecture of land plant bodies is determined by the orientation of cell walls deposited between dividing nuclei in developing organs. The ability to determine cell wall orientation in various planes was a novel feature that evolved in the transition from filamentous charophyte algae to the first land plants ( Pires and Dolan, 2012). In contrast to land plants, tip expansion and fixed division planes of the cells drove a two-dimensional (2D) growth pattern of charophyte members represented by filamentous or discoid forms. The membrane-anchored DEFECTIVE KERNEL1 (DEK1) calpain is a candidate protein involved in position-dependent cell wall deposition in the plant lineage (Perroud et al., 2014). Evidence for a conserved function in land plants comes from the observation that DEK1 is essential for cell wall orientation in land plants ranging from angiosperms to mosses, an evolutionary time span of 450 million years (Olsen et al., 2015). Support for a central function of DEK1 also comes from a recent review in which analyses of meristematic shoot tip transcriptomes of widely divergent extant vascular plant lineage members suggest that different genes were recruited to regulate similar meristematic processes during evolution. Exceptions identified include PIN-FORMED, DEK1, and LONELY GUY1 (Frank et al., 2015) proteins that represent potential genetic homologs in meristem function (Harrison, 2015).The DEK1 calpain derives its name from the maize (Zea mays) mutant dek1, whic...

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An experimental method to facilitate the identification of hybrid sporophytes in the moss Physcomitrella patens using fluorescent tagged lines

Cited by 64 publications

References 27 publications

Sporophyte formation and life cycle completion in moss requires heterotrimeric G-proteins

Sporophyte formation and life cycle completion in moss requires heterotrimeric G-proteins

Balance between inbreeding and outcrossing in a nannandrous species, the moss Homalothecium lutescens

The DEK1 calpain Linker functions in three-dimensional body patterning in Physcomitrella patens

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