An Experimental Model for Hyaline Membrane Disease

On the pulmonary toxicity of oxygen. 4. The thyroid arena

“…They were maintained there until 24 hours of age without caloric supplementation. At 24 hours all animals were subjected to bilateral cervical vagotomy as previously described (Shanklin and Berman, 1964;Shanklin and Sotelo-Avila, 1967). Almost all vagotomies took less than one minute to complete, including positioning and restraining the subject for the procedure.…”

“…Almost all vagotomies took less than one minute to complete, including positioning and restraining the subject for the procedure. A few drops of dilute local anesthetic were used in all cases (Shanklin and Berman, 1964;Shanklin and Sotelo-Avila, 1967).…”

“…Animals were removed after death and were autopsied according to the methods and procedures previously reported (Shanklin and Berman, 1964;Shanklin and Sotelo-Avila, 1967;Shanklin, 1969). In brief, the extent of lesion was determined by a grid approximation .formula on the pleural surface of the lungs, with each of the four principal lobes considered separately.…”

“…The sex ratio of individual groups was left to random sort by the minimum test group size as noted above (Shanklin and Berman, 1964;Shanklin and SoteloAvila, 1967;Shanklin, 1969) since the sex of the newborn animal is readily determined only at autopsy.…”

“…The model is based on the effects of bilateral cervical vagotomy (BCV) on the lung (Farber, 1937). When Farber's model using adult rabbits is transferred to the newborn rabbit the resulting lesion is that of human neonatal hyaline membrane disease (Shanklin and Berman, 1964 ). The frequent occurrence of intermediate severity of the lesion in the model offered a way to ascertain certain pathogenetic factors of importance.…”