An experimental model: intrauterine adhesion versus subendometrial fibrosis

Abstract

Background: Intrauterine adhesions (IUA) remain a major cause of infertility. The prevalence of IUA varies geographically and keeps increasing over the last few decades due to increased hysteroscopic surgeries. Therefore, IUA management has received considerable attention. However, the management of IUAs still presents a big challenge: the recurrence rate could be up to 62.5% in severe IUAs. Intrauterine adhesion (IUA) occurs as a result of endometrial destruction by surgical interventions or infection causing obliteration of uterine cavity by the adhesions which interfere with embryonicimplantation. Sub endometrial fibrosis may occurs, which causes narrowing of uterine cavity so no enough space for fetal growth. This leads to recurrent abortion. Aim: We aim in this experiment to apply a method for two models of IUA and or fibrosis by intrauterine injections of two different doses of trichloroacetic acid. Method: This experimental study was performed on 30 albino adult rats which were divided into three groups (n=10 rats/group) as follows, group 1: normal control rats, group 2: induced IUA and/or fibrosis that received low dose trichloroacetic acid, group 3: induced fibrosis that received high dose trichloroacetic acid, the extent of fibrosis, vascularization and inflammation were evaluated by; qRT-PCR for IL-6, TNF, VEGF and TG-β immunohistochemistry examination for VEGF and TGF-β and histological assessment. Results: We found significant increase in IL-6, TNF and TGF in high dose group and significant decrease in VEGF in high dose group compared to normal group and low dose group (p<0.05). Discussion: The reproductive cells are the most sensitive to toxic environmental materials. In this study, we found that group II rats (IUAs), unlike control rats, had impaired endometrial epithelial cells, a lower number of endometrial glands, higher inflammatory cell infiltration, poor vascularity, and a severe narrowing of the uterine cavity with dense endometrial fibrosis as confirmed by H&E and Masson's trichrome staining.

Inhibitory activity of flaxseed oil against CdCl2 induced liver and kidney damage: Histopathology, genotoxicity, and gene expression study

“…The sequences of the specific primers used are listed in Table 1 . The following thermal cycling conditions were applied: 50 °C for 2 min, then 95 °C for10 min followed by 40 cycles of 95 °C for 30 s, 60 °C for 30 s, and 72 °C for 30 s. Changes in the expression of each target mRNAs were normalized relative to the mean critical threshold values of GAPDH mRNA(internal reference) using the ΔΔCt method [ 25 ].…”

Insulin-like Growth Factor Initiates Hepatocellular Carcinoma in Chronic Hepatitis C Virus Patients through Induction of Long Non-coding Ribonucleic Acids AF085935

“…The PCR run was performed on a Rotor Gene Real-Time PCR System (Qiagen). Normalization for variation in the expression of each target gene was performed referring to the mean critical threshold (CT) values of ß-actin housekeeping gene expression by the ΔΔCt method [18]. Primers sequence for all studied genes was listed as following in Table 1.…”

Protective Role of Ficus carica Extract Against Hepato-Testicular Side Effects and Genotoxicity Induced by Cisplatin

“…To remove any possible genomic DNA contamination, the total RNA samples were pre-treated using DNA-free™ DNase treatment and removal of reagents kit (Ambion, Austin, TX, USA) following the manufacturer's protocol and were recovered in 100 μl molecular biology grade water and stored at -20°C. The RNA concentration and purity were determined by Nanodrop Spectrophotometer absorption (Thermo Scientific, USA) at 260 nm 16 .…”