Local particle deposition measurements in a T-junction of a real scale ventilation network are studied and interpreted using RANS CFD simulations. To assess the validity of the simulation, an analysis of the flow at different scales is proposed. The experimental results show a particle deposition tendency to be higher downstream the T-junction and particularly on the outer side of the T-junction. This tendency appears to be linked to a separation downstream the T-junction, with the main flow on the outer side of the junction and secondary flow on the inner side. This separation seems to have an impact on the aerosol repartition in the bulk, which can be linked to aerosol concentrations gradient downstream the T-junction.