An Experimental Steroid Responsive Model of Ocular Inflammation in Rabbits Using an SLT Frequency Doubled Q Switched Nd:YAG Laser

Gulati, Vikas; Pahuja, Sandhya; Fan, Shan; Toris, Carol B.

doi:10.1089/jop.2012.0223

Cited by 6 publications

(8 citation statements)

References 27 publications

(23 reference statements)

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“…Increased levels of lipid peroxidase in aqueous humor and free oxygen radicals that are released from trabecular meshwork have been suggested to take part in inflammation occurring after SLT application [ 21 ]. In an animal experiment by Gulati et al [ 22 ] that investigated response to steroid treatment in anterior uveitis which was developed by applying laser to anterior surface of iris with SLT device, histopathological examinations revealed that there was pigment clumping at anterior surface of iris stroma, and that the changes were limited to anterior layers of iris. Additionally, after measurements conducted with flarimetry, they reported that laser applications resulted in anterior chamber inflammation that was responsive to steroids [ 22 ].…”

Section: Discussionmentioning

confidence: 99%

“…In an animal experiment by Gulati et al [ 22 ] that investigated response to steroid treatment in anterior uveitis which was developed by applying laser to anterior surface of iris with SLT device, histopathological examinations revealed that there was pigment clumping at anterior surface of iris stroma, and that the changes were limited to anterior layers of iris. Additionally, after measurements conducted with flarimetry, they reported that laser applications resulted in anterior chamber inflammation that was responsive to steroids [ 22 ]. In our study, we observed ciliary injection and mild anterior chamber reaction in all groups on the first day, being more prominent in the Group B in which higher dose of laser was applied; and these signs disappeared in the examination at first week.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of Color-Changing Effect and Complications After Nd: YAG Laser Application On Iris Surface

et al. 2016

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BackgroundThe aim of this study was to evaluate the color-changing effect and adverse effects after Nd: YAG laser application on the iris surface of rabbit eyes.Material/MethodsThe study was performed on right eyes of 12 pigmented rabbits. A laser device that produces frequency doubled 532 nm wavelength Nd: YAG laser with 900 μm spot diameter was used. The laser was applied in 3 sessions at 2-week intervals, at energy levels of 0.8 mJ in Group A and 1.5 mJ in Group B. Slit-lamp examinations and measurements of intraocular pressure (IOP) using a Tono-Pen were performed before and 1 day after each laser session. Iris thickness (IT) was measured at the beginning and the end using an ultrasonic biomicroscope. The eyes were enucleated for histopathologic examination on day 60.ResultsOn the first day after each laser session, maximum grade 1 anterior chamber flare and cells were observed in both groups. In all eyes, flare and cells disappeared at the end of the first week. There was no significant difference in the IOP and IT values between measurements performed prior to and after laser sessions during the study (p>0.05). None of the eyes showed complications such as corneal edema, hypopyon, posterior synechia, transillumination defect, or pupillary defect. In histopathological examinations, reduction in pigment density was more profound in Group B compared to Group A, which was statistically significant (p<0.019).ConclusionsThere were no serious complications apart from mild transient inflammatory signs. Change in iris color was more evident at the end of the second month.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Evaluation of Color-Changing Effect and Complications After Nd: YAG Laser Application On Iris Surface

et al. 2016

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“…SLT induces minimal structural changes in the trabecular meshwork [1] in contrast to argon laser trabeculoplasty. Therefore, the liberation of humoral factors is thought to play a significant role in IOP reduction, which was also observed in animal models involving rabbits [2,3]. There were a few animal studies to suggest that the chemical components of the aqueous was modified after SLT [4,5].…”

Section: Introductionmentioning

confidence: 93%

Intraocular Pressure Elevation in the Contralateral Untreated Eye Following Selective Laser Trabeculoplasty in Rabbit Eyes

Choy¹,

Chiu²

2015

J Clin Exp Ophthalmol

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Our study aimed at evaluating if a single session of unilateral selective laser trabeculoplasty would affect the intraocular pressure of the fellow untreated eye in rabbits. Eleven rabbits were involved. 360° selective laser trabeculoplasty with 532 nm frequency-doubled green Nd:YAG laser was performed over the right eyes. The mean intraocular pressure of both eyes at baseline, 3 hours, 24 hours, 3 days and 7 days after laser were measured. The baseline mean intraocular pressure of the right eye and the left eye were 8.07 ± 1.72 mmHg and 8.27 ± 1.56 mmHg respectively (p=0.78). The mean intraocular pressure of the treated eye was lower than the baseline from 3 hours through 3 days after laser, with a maximum mean decrease of 1.36 mmHg on Day 3. On the contrary, the mean intraocular pressures of the untreated eyes were higher than the baseline throughout the study, particularly at later course (1.91 mmHg on Day 3 and 1.85 mmHg on Day 7). This suggests the change of intraocular pressure in one eye after selective laser trabeculoplasty, leads to a change in the pressure of the fellow eye. It is thought that a neuronal and humoral response is triggered centrally after unilateral intraocular pressure change. The detailed mechanisms would require further studies to evaluate. The rising intraocular pressure trend in the contralateral untreated eye suggests that response may continue to exert its action for some time after the initial laser. This is the first reported study of intraocular pressure elevation of the untreated eye following contralateral selective laser trabeculoplasty.

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“…На відміну від загальноприйнятої терапії увеїтів (Jamieson, Meckoll-Brinck, & Keller, 1989;Shelley et al, 2012;Gulati, Pahuja, Fan, & Toris, 2012;Movafagh, Heydary, Mortazavi-Tabatabaei, & Azargashb, 2011;Ahmad, 2018) з використанням кортикостероїдів, застосування яких супроводжується рядом небажаних побічних ефектів (гальмування регенерації, пригнічення імунної функції тощо), провели апробацію лікування токсикоалергічного увеїту із використанням нестероїдних протизапальних препаратів.…”

Section: вступunclassified

Modelling and treatment of the uveitis in rabbit

Doroshchuk¹

2019

VTTP

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Modelling of the toxico-allergic uveitis is better conducted on rabbits. For the treatment, contrary to corticosteroids that generate a number of side effects (e.g. suppression of reaction, immune inhibitions etc.), one can successfully use nonsteroid drugs, particularly Voltaren and Vetofluxin, which render phagocytes stimulation and immunoglobulin control. As a result, use of these drugs brings to recovery within 7 days. The decrease in the level of immunoglobulins in the blood serum during the treatment of uveitis compared with levels untreated animals was accompanied by a sharp attenuation of the inflammatory response in the blood test and taking into account the features of immunological protection of the eye (the presence of blood-ocular barrier between blood and chamber moisture, vascular membrane, retina, vitreous body) it should be emphasized that the body as a whole responds to the inflammatory process in the uveal tract. Considering toxico-allergic uveitis as an autoimmune disease and in respect that the positive effects of the drugs used on inflammatory and allergic processes, we can predict that the drugs we use are able to participate in the regulation of antibody-forming function of Bcells and inhibite autoimmune disorders. Both treatment schemes show the stimulating effect on phagocytosis, comparing with using phytohemagglutinin, which show no effect. In experimental toxico-allergic uveitis, the treatment regimen in the first experimental group not only did not reduce, but moreover, retained the potential effect on the phagocytic activity of leukocytes. Compared with phytohemagglutinin impact on phagocytosis, found that both represented treatment strategies stimulate the phagocytosis. In experimental toxico-allergic uveitis, Voltaren and Vetofluxin not only did not reduce, as is usually the case with corticosteroids, but moreover, clearly showed a stimulating effect on the phagocytic activity of peritoneal macrophages. Thus, studies have shown that in the treatment of such a severe inflammatory process as uveitis, in contrast to corticosteroids, the use of which is accompanied by a number of undesirable effects (inhibition of regeneration, inhibition of immune reactions, etc.), you can successfully use non-steroidal anti-inflammatory drugs, in particular Voltaren and Vetofluxin. The stimulating phagocytosis effect of Voltaren and Vetofluxin and ability to regulate the levels of immunoglobulins during inflammation has been established.

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An Experimental Steroid Responsive Model of Ocular Inflammation in Rabbits Using an SLT Frequency Doubled Q Switched Nd:YAG Laser

Abstract: Commercially available SLT laser can be used to create a minimally invasive, steroid-responsive animal model of anterior uveitis with the potential for use in the evaluation and comparison of drugs intended to treat AC inflammation.

Cited by 6 publications

References 27 publications

Evaluation of Color-Changing Effect and Complications After Nd: YAG Laser Application On Iris Surface

Evaluation of Color-Changing Effect and Complications After Nd: YAG Laser Application On Iris Surface

Intraocular Pressure Elevation in the Contralateral Untreated Eye Following Selective Laser Trabeculoplasty in Rabbit Eyes

Modelling and treatment of the uveitis in rabbit

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