An Experimental Study of Intramammary Injection of Au198

Berg, Harold F.; Christophersen, William M.

doi:10.1097/00000658-195601000-00012

Cited by 3 publications

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“…Decreased anticancer activity of docetaxel-loaded NLC and the NLC-loaded gel in comparison to docetaxel as observed in our experiments is also due to slow rate of uptake of nanolipid carriers, and the docetaxel release from these NLCs is the critical factor for the drug uptake by cells. Colloidal delivery systems like NLCs administered by intramammary injection could yield high local concentration in the breast tissue and its associated lymph drainage area (Berg and Christophersen 1956 ). Similar conclusions were also drawn by Mendes et al ( 2009 ) based on their work on the uptake by breast carcinoma and lymph nodes of a lipidic nanoemulsion after intralesional injection into the patients.…”

Section: Discussionmentioning

confidence: 99%

Nanolipid carrier-based thermoreversible gel for localized delivery of docetaxel to breast cancer

et al. 2013

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Intratumoral and intralesional administration of anticancer drugs in gels and implantable formulations is gaining much importance on account of its advantage of site-specific delivery with highly dependable freedom from unwanted side effects. Nanolipid carriers (NLC) are the preferred vehicle due to their improved properties particularly drug loading. In the present investigation, glyceryl monostearate–oleic acid NLCs loaded with docetaxel were prepared by emulsification and ultrasonication technique and were incorporated in thermoreversible pluronic F127 gel (TRPgel) for intralesion injection to breast tissue. The NLCs were spherical particles of 113 nm size with a negative zeta potential of −32.8 and 85 % drug entrapment. In vitro drug release profile of the NLC showed 96 % drug release in 48 h following Higuchi release kinetics. NLC incorporated TRPgel showed mucoadhesive force of 3.07 dynes/cm2 and gelling temperature in the range of 32 to 37 °C. The drug entrapped gel was also subjected to in vitro cytotoxicity study in human B-16 and HeLa cell lines by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and in vivo drug distribution study in breast tissue in healthy Wistar rats. The MTT assay revealed that docetaxel-loaded NLC incorporated into gel showed lower cytotoxicity than docetaxel. However, in vivo breast tissue distribution studies showed high tissue drug concentration, sustained over a period of 60 h in comparison to docetaxel and docetaxel-loaded NLCs. These results suggest that nanolipid carrier of docetaxel in TRPgel could be a promising carrier system to deliver drug to tumor by intralesional administration for improving therapeutic benefits of docetaxel.

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Section: Discussionmentioning

confidence: 99%