In order to understand the characteristics and influencing factors of surface release fluxes in the typical agro-pastoral mosaic region of the Songnen grasslands of northern China, this study selected two sample plots to, respectively, represent the L. chinensis covered grassland (L), and the Z. mays covered fields (Z) in the Waizi area of Changling County, with western Jilin Province as the study area. This study investigated the atmospheric mercury (Total Gaseous Mercury (TGM)) concentrations, surface soil mercury concentrations, the gaseous elemental mercury exchange fluxes, and related environmental factors. The results showed that the TGM concentrations in two sample plots in the study area were basically the same during the summer and autumn months, but were higher in summer than in autumn, and there was a clear diurnal pattern. The average mercury concentrations in the surface soil were also higher in summer than in autumn. Furthermore, the surface soil has a clear mercury release process, and the mercury release were higher in the sheep grass field than the maize field during the same season. The mercury release fluxes in each site within the ecotone were related to the solar radiation and soil temperature values, and the effects of them on the soil mercury release were independent from one another.