In epidural anesthesia, it is difficult to specify the puncture position of the anesthesia needle. We have proposed an ultrasonic method to depict the thoracic spine using the different characteristics of reflection from bone and scattering from muscle tissue. In the present paper, we investigated the transmission aperture’s width of the ultrasound probe to emphasize the differences in the reflection and scattering characteristics. First, we determined the optimum transmission aperture’s width using a simulation experiment. Next, we measured reflection and scattering signals by changing the transmission aperture’s width in a water tank experiment and confirmed that the results corresponded to the simulations. However, as the transmission aperture’s width increased, the lateral resolution at the focal point improved. Therefore, better imaging of the human thoracic vertebrae can be achieved by selecting the transmission aperture’s width, which considers the effect on lateral resolution.