This study aims to evaluate the effectiveness of a natural biological treatment system for wastewater using Red soil, Arka Darshan, Arka Tilak, Arka Nirantara, and Arka Prajwal plants. The system employs clay as a natural nanomaterial for adsorption of substances such as nitrates, phosphates, potassium, and heavy metals. The soil contains numerous microorganisms that decompose organic matter into simpler compounds, while the plants use phytoremediation to uptake heavy metals and nutrients. The study measured the system’s efficiency in reducing Biochemical Oxygen Demand (BOD), Chemical Oxygen Demand (COD), Total Suspended Solids (TSS), Total Dissolved Solids (TDS), Phosphate, Magnesium, Zinc, Nitrate, Sodium, and Potassium. The results showed that the system had an average efficiency of 98% in COD and 96% in BOD removal, and contributed an efficiency of 99% TSS and 46% TDS. The system also removed nitrates and phosphates, which cause eutrophication in water bodies, by 76% and 72%, respectively. Additionally, heavy metals like chromium, Nickel and Zinc were reduced by up to 81%, 71% and 59%. The study suggests that the natural biological treatment system is a sustainable and effective alternative to conventional wastewater treatment, particularly in sparsely populated rural areas. Therefore, we can conclude that this system has potential for wider adoption in treating wastewater.