An explanation for nonimmunologic adsorption of proteins onto red blood cells: Schiff's base reactions

Jamin, Daniela; Demers, J. M.; Shulman, Ira A.; Lam, H Y; Momparler, Richard L.

doi:10.1182/blood.v67.4.993.993

Cited by 15 publications

(4 citation statements)

References 3 publications

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“…Jamin et al (1986 ) showed that diglycoaldehyde (INOX), an intravenous chemotherapeutic agent used to treat childhood malignancies, caused positive DATs in all eight patients receiving the drug. IgG and albumin were found to bind to human RBCs when they were incubated in vitro with normal plasma and INOX, or glutaraldehyde, which is similar in structure to INOX.…”

Section: Discussionmentioning

confidence: 99%

Positive direct antiglobulin tests and haemolytic anaemia following therapy with beta‐lactamase inhibitor containing drugs may be associated with nonimmunologic adsorption of protein onto red blood cells

Garratty

Arndt

1998

Br J Haematol

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Summary.A high incidence (39%) of positive direct antiglobulin tests (DATs) has been reported in patients taking Unasyn [ampicillin sodium plus sulbactam sodium (a beta-lactamase inhibitor)]. Three of four patients, with positive DATs, receiving Unasyn or Timentin [ticarcillin disodium plus clavulanate potassium (also a beta-lactamase inhibitor)] developed a haemolytic anaemia (HA) associated with a positive DAT, which resolved when drug therapy was stopped. The patients' sera did not react with red blood cells (RBCs) in the presence of Unasyn or Timentin, but when drug-treated RBCs were tested, patients' sera and normal sera reacted equally by indirect antiglobulin test. Following incubation in normal sera, RBCs treated with Unasyn, Timentin, Augmentin (amoxicillin þ clavulanate), sulbactam and clavulanate reacted with anti-human globulin and anti-human albumin (an index of non-specific adsorption); RBCs treated with ampicillin and amoxicillin were nonreactive.The beta-lactamase inhibitors sulbactam and clavulanate seem to cause nonimmunologic adsorption of protein onto RBCs in vitro. This may explain the high incidence of positive DATs detected in patients taking Unasyn, which contains sulbactam. It was not possible to prove that there was a direct association between the nonspecific uptake of protein onto drug-treated RBCs in vitro with the positive DATs or the HA.

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Section: Discussionmentioning

confidence: 99%

Positive direct antiglobulin tests and haemolytic anaemia following therapy with beta‐lactamase inhibitor containing drugs may be associated with nonimmunologic adsorption of protein onto red blood cells

Garratty

Arndt

1998

Br J Haematol

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“…Some other drugs also appear to do the same [e.g. diglycoaldehyde (INOX) (Jamin et al ., 1986), suramin (Jamin et al ., 1986), cisplatin (Getaz et al ., 1980; Levi et al ., 1981; Nguyen & Jaffe, 1981; Cinollo et al ., 1988; Zeger et al ., 1988 and Weber et al ., 1990) and β‐lactamase inhibitors (e.g. clavulanate and sulbactam)].…”

Section: Discussionmentioning

confidence: 99%

Tazobactam‐induced haemolytic anaemia, possibly caused by non‐immunological adsorption of IgG onto patient's red cells

Broadberry

Farren

Bevin

et al. 2004

Transfusion Medicine

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A patient with pneumonia was treated with Tazocin (piperacillin/tazobactam). However, the expected haemoglobin (Hb) increment after transfusion was not achieved. Plasma bilirubin and lactate dehydrogenase were raised. The direct antiglobulin test (DAT) was positive (4+) for immunoglobulin G (IgG) only, but no RBC antibodies were demonstrable in the plasma or an eluate from the patient's RBCs. Drug-induced haemolysis was suspected. After discontinuing Tazocin administration, Hb and bilirubin levels returned to expected values. The patient's plasma gave a positive (3+) indirect antiglobulin reaction only with RBCs pretreated with tazobactam. However, random patient plasmas also gave weak (+/- to 1+) reactions, indicating non-immunological adsorption of IgG onto RBCs rather than specific anti-tazobactam antibodies. Subsequently, plasma samples with varying IgG levels (0.8-89.7 g L(-1)) were tested against RBCs pretreated with tazobactam. The amount of plasma IgG non-immunologically adsorbed onto the drug-coated RBCs was found to correlate directly with the plasma IgG level. The patient had a high plasma IgG level (41.6 g L(-1)) which explains why the antiglobulin test was stronger with the patient's plasma than with random plasma samples. Previous reports (Garratty & Arndt, (1998) British Journal of Haematology, 100, 777-783; Arndt & Garratty (2000) Transfusion, 40, 29S) suggested that non-immunological coating of RBCs with IgG may affect RBC survival; our results would support that suggestion. This is the first reported case of haemolytic anaemia associated with tazobactam.

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“…As reported in the literature, the Glu coupling reaction on chip was performed at a pH of 7.4, a slightly alkaline environment, as a lower pH than this would reduce the efficiency of the coupling reaction. 33 Attachment of gold nanoparticle conjugated-streptavidin on sensor chips: SEM and waveguide sensor analyses Nanomaterials can facilitate signal transduction just like fluorophores or electroactive tags and be useful for imaging studies. GNP is the ideal nanomaterial commonly used in the sensor developments, has unique characteristics, such as easy to disperse in water, compatible with surface functionalization to link biomolecules and can be tailored with the desired nano-sizes.…”

Section: Surface Functionalization Chemistry On Waveguide Sensor Chipsmentioning

confidence: 99%

Surface functionalization chemistries on highly sensitive silica-based sensor chips

Gopinath

Awazu

Fujimaki

et al. 2012

Analyst

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The surfaces of silica-based sensor chips, designed for evanescent-field-coupled waveguide-mode sensors, were functionalized using various surface chemistries. The immobilization of molecular entities on the functionalized silica surfaces was monitored using various microscopic techniques (scanning electron, fluorescence, and atomic force microscopies). Further, gold nanoparticle-based signal enhancement analyses were performed with protein conjugation on different functionalized surfaces using a waveguide-mode sensor. Based on these analyses, the sensor surfaces modified with glutaraldehyde (Glu) and carbonyldiimidazole were found to be good for molecules of different sizes. In addition, it can be inferred that the Glu-modified surface may be suitable for small molecules with diameters around 5 nm owing to its surface roughness. The modified surface with carbonyldiimidazole is suitable for the direct immobilization of larger molecules especially for biomolecular assemblies without intermediate chemical modifications.

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An explanation for nonimmunologic adsorption of proteins onto red blood cells: Schiff's base reactions

Cited by 15 publications

References 3 publications

Positive direct antiglobulin tests and haemolytic anaemia following therapy with beta‐lactamase inhibitor containing drugs may be associated with nonimmunologic adsorption of protein onto red blood cells

Positive direct antiglobulin tests and haemolytic anaemia following therapy with beta‐lactamase inhibitor containing drugs may be associated with nonimmunologic adsorption of protein onto red blood cells

Tazobactam‐induced haemolytic anaemia, possibly caused by non‐immunological adsorption of IgG onto patient's red cells

Surface functionalization chemistries on highly sensitive silica-based sensor chips

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