An explanation for the relationship between salt‐induced thylakoid stacking and the chlorophyll fluorescence changes associated with changes in spillover of energy from photosystem II to photosystem I

Barber, James

doi:10.1016/0014-5793(80)81207-5

Cited by 247 publications

(102 citation statements)

References 61 publications

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“…As can be seen in Table 2, transition to state II in maize is accompanied by a slight destacking, even though we used a high Mg2" concentration in our in vitro experiments (10 mM, to which we should substract the 1 mM needed to complex the ATP added). This change is of similar amplitude to that seen in pea at 5 mM Mg2" (33) and may be understood in the frame of the electrostatic theory of stacking (34) as reflecting the increased amount of proteins that are excluded from stacked regions, even at maximum electrostatic screening. Such a destacking can by itself increase the proportion of cyt b6/f in unstacked regions and, therefore, its proximity with PSI, with no need for a change in the relative affinity of cyt b6/f for unstacked regions.…”

Section: Discussionsupporting

confidence: 54%

Lateral redistribution of cytochrome b6/f complexes along thylakoid membranes upon state transitions.

Vallon

Bulté

Dainese

et al. 1991

Proc. Natl. Acad. Sci. U.S.A.

190

130

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Although the changes in organization of the lightharvesting antenna upon state transitions are well documented, possible changes in the organization of the photosynthetic electron transfer chain have not been directly investigated. Cytochrome b6/f (cyt b6/f), a major protein complex of this electron-transfer chain, has, however, been implicated in state transitions through its role in LHCIIkinase activation. State transitions are abolished in cyt b6/f mutants of green algae and higher plants due to the absence of LHCII reversible phosphorylation (4-8). Gal et al. (9) recently reported that the LHCII-kinase was, indeed, associated with cyt b6/f complexes.Whereas the PSII and PSI centers are well separated between the stacked and unstacked regions of the thylakoid membranes, cyt b6/f complexes are found in significant amounts in both membrane domains (10-13). The identity of the long-distance carrier between PSIl in the grana regions and PSI in the SL regions has been a matter of debate (14). It has been recently argued that the rapid diffusion ofplastoquinones, which transfer electrons between PSII and cyt b6/f complexes, is limited to small domains containing less than eight PSII centers (15,16). Therefore linear electron flow should be sustained by plastocyanin diffusing in the luminal space from its binding site on cyt b6df complexes in the stacked regions to PSI in the unstacked regions. The fraction of cyt b6/f complexes located in the unstacked regions next to PSI would then serve cyclic electron flow around PSI.There is a growing body of evidence that the ATP requirement of the photosynthetic cell controls state transitions (17)(18)(19) 8262The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.

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Section: Discussionsupporting

confidence: 54%

Lateral redistribution of cytochrome b6/f complexes along thylakoid membranes upon state transitions.

Vallon

Bulté

Dainese

et al. 1991

Proc. Natl. Acad. Sci. U.S.A.

190

130

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“…These reversible responses to changes in the wavelength of light are known as State 1-State 2 transitions and are regarded as being important in ensuring the balanced delivery of excitation energy to the two photosystems under natural conditions . They are observed both in algae and in higher plants (Chow et al, 1981;Barber, 1982 (Murata, 1969b;Williams, 1977;Barber, 1980Barber, , 1982. A characteristic feature of most green plants is that their photosynthetic membranes are differentiated into appressed and non-appressed regions (Fig.…”

Section: Phytochrome Controls the Lhcp Mrna Levelsmentioning

confidence: 99%

Regulation of photosynthesis by reversible phosphorylation of the light-harvesting chlorophyll a/b protein

Bennett¹

1983

Biochemical Journal

272

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“…Unstacking of the thylakoid membrane by cation-depletion is known to increase the PSI fluorescence emission (e.g. Barber 1980;Briantais et al 1984;Williams et al 1987;Stoitchkova et al 2006). Both Briantais and co-workers and Stoitchkova and co-workers assigned this increase to spillover from PSII to PSI, as a result of the induced homogeneous distribution of both photosystems by unstacking.…”

Section: Introductionmentioning

confidence: 99%

Excitation energy transfer in native and unstacked thylakoid membranes studied by low temperature and ultrafast fluorescence spectroscopy

et al. 2007

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In this work, the transfer of excitation energy was studied in native and cation-depletion induced, unstacked thylakoid membranes of spinach by steady-state and time-resolved fluorescence spectroscopy. Fluorescence emission spectra at 5 K show an increase in photosystem I (PSI) emission upon unstacking, which suggests an increase of its antenna size. Fluorescence excitation measurements at 77 K indicate that the increase of PSI emission upon unstacking is caused both by a direct spillover from the photosystem II (PSII) core antenna and by a functional association of light-harvesting complex II (LHCII) to PSI, which is most likely caused by the formation of LHCII-LHCI-PSI supercomplexes. Time-resolved fluorescence measurements, both at room temperature and at 77 K, reveal differences in the fluorescence decay kinetics of stacked and unstacked membranes. Energy transfer between LHCII and PSI is observed to take place within 25 ps at room temperature and within 38 ps at 77 K, consistent with the formation of LHCII-LHCI-PSI supercomplexes. At the 150-160 ps timescale, both energy transfer from LHCII to PSI as well as spillover from the core antenna of PSII to PSI is shown to occur at 77 K. At room temperature the spillover and energy transfer to PSI is less clear at the 150 ps timescale, because these processes compete with charge separation in the PSII reaction center, which also takes place at a timescale of about 150 ps.

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An explanation for the relationship between salt‐induced thylakoid stacking and the chlorophyll fluorescence changes associated with changes in spillover of energy from photosystem II to photosystem I

Cited by 247 publications

References 61 publications

Lateral redistribution of cytochrome b6/f complexes along thylakoid membranes upon state transitions.

Lateral redistribution of cytochrome b6/f complexes along thylakoid membranes upon state transitions.

Regulation of photosynthesis by reversible phosphorylation of the light-harvesting chlorophyll a/b protein

Excitation energy transfer in native and unstacked thylakoid membranes studied by low temperature and ultrafast fluorescence spectroscopy

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