An explorative study comparing levels of soluble mediators in control and osteoarthritic synovial fluid

Beekhuizen, M.; Gierman, Lobke; Spil, W.E. van; Osch, Gerjo J. V. M. van; Huizinga, Tom W J; Saris, Daniël B.F.; Creemers, Laura B.; Zuurmond, Anne‐Marie

doi:10.1016/j.joca.2013.04.002

Cited by 113 publications

(111 citation statements)

References 11 publications

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“…[20,33,34] In order to examine whether synovial fluid levels of leptin are affected in OA, we assessed synovial fluid leptin concentrations in individuals with and those without OA. Our results indicate that, at least in women and similar to IGF-1, both unadjusted as well as protein-normalized leptin levels are significantly higher in OA compared to normal.…”

Section: Discussionmentioning

confidence: 99%

Women with Osteoarthritis have Elevated Synovial Fluid Levels of Insulin-Like Growth Factor (IGF)-1 and IGF-Binding Protein-3

Hooshmand

Juma

Khalil

et al. 2014

Journal of Immunoassay and Immunochemistry

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The present study explores the possible connection between synovial fluid concentrations of insulin like growth factor (IGF-1), IGF-binding protein (IGFBP-3), leptin, and C-reactive protein (CRP) in osteoarthritis (OA). Synovial fluid specimens were obtained from a total of thirty-four individuals with and without OA. Protein-normalized measurements of IGF-1, IGFBP-3, and leptin concentrations in synovial fluid showed significantly (P < 0.05) elevated levels in women with knee OA but not in men. This study provides initial evidence that protein normalized IGF-1 and IGFBP-3 and leptin levels increase in synovial fluid of women but not in men with OA versus those without OA.

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Section: Discussionmentioning

confidence: 99%

Women with Osteoarthritis have Elevated Synovial Fluid Levels of Insulin-Like Growth Factor (IGF)-1 and IGF-Binding Protein-3

Hooshmand

Juma

Khalil

et al. 2014

Journal of Immunoassay and Immunochemistry

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“…Beekhuizen et al (2013) analysed the presence of inflammatory mediators in knee synovial fluid. Inflammatory mediators were detected by immunoenzymatic assays (ELISA).…”

Section: Occurrence Of Cx3cl1 and Its Receptor Cx3cr1 In Affected Tmentioning

confidence: 99%

The Chemokine CX3CL1 (Fractalkine) and its Receptor CX3CR1: Occurrence and Potential Role in Osteoarthritis

Wojdasiewicz

Poniatowski

Kotela

et al. 2014

Arch. Immunol. Ther. Exp.

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Chemokines are molecules able to induce chemotaxis of monocytes, neutrophils, eosinophils, lymphocytes and fibroblasts. The complex chemokine acts in many physiological and pathological phenomena, including those occurring in the articular cartilage. To date, chemokine CX3CL1 (fractalkine) is the only member of the CX3C class of chemokines with well-documented roles in endothelial cells. CX3CL1 is a unique chemokine that combines properties of chemoattractant and adhesion molecule. The main roles of CX3CL1 include promotion of leukocyte binding and adhesion as well as activation of the target cells. The soluble chemokine domain of CX3CL1 is chemotactic for T cells and monocytes. CX3CL1 acts via its receptor, CX3CR1, which belongs to a family of G protein-coupled receptors. Stimulation of CX3CR1 activates both CX3CL1-dependent and integrin-dependent migrations of cells with synergistically augmented adhesion. Genetic polymorphisms of CX3CR1 may significantly modify the biological roles of CX3CL1, especially in pathologic conditions. Osteoarthritis (OA) is the most common joint disease, affecting approximately 7–8 % of the general population. Development of OA is largely driven by low-grade local background inflammation involving chemokines. The importance of CX3CL1/CX3CR1 signalling in the pathophysiology of OA is still under investigation. This paper, based on a review of the literature, updates and summarises the current knowledge about CX3CL1/CX3CR1 in OA and indicates possible interactions with a potential for therapeutic targeting.

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“…36 We examined whether low concentrations of IL-1b and TNF-a would influence the chondrogenic differentiation potential. Studies have shown that synovial fluid levels of IL-1b and TNF-a vary widely in osteoarthritic patients, from 4.8 pg/mL 37 38 for TNF-a. Our results indicate that 10 pg/mL of IL1b and 1 pg/mL of TNF-a are sufficient to modulate the expression of chondrogenic-related genes, suggesting that IL-1b and TNF-a in osteoarthritic synovial fluid might influence the chondrogenic differentiation potential.…”

Section: Cd14 and Proinflammatory Cytokines On Chondrogenesis In Oa Smentioning

confidence: 99%

Effects of CD14 Macrophages and Proinflammatory Cytokines on Chondrogenesis in Osteoarthritic Synovium-Derived Stem Cells

Sun

Lee

Seong

et al. 2014

Tissue Engineering Part A

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We investigated the effects of CD14 macrophages and proinflammatory cytokines on chondrogenic differentiation of osteoarthritic synovium-derived stem cells (SDSCs). Osteoarthritic synovial fluid was analyzed for interleukin-1b (IL-1b), tumor necrosis factor-a (TNF-a), and IL-6. Levels of stem cell surface markers in osteoarthritic SDSCs were evaluated using flow cytometry. CD14-negative cells were obtained using magnetically activated cell sorting. We compared chondrogenic potentials between whole cells and CD14-negative cells in CD14 low cells and CD14 high cells, respectively. To assess whether nuclear factor-kB (NF-kB) and CCAAT/enhancer-binding protein b (C/EBPb) modulate IL-1b-induced alterations in chondrogenic potential, we performed small interfering RNA transfection. We observed a significant correlation between the CD14 ratio in osteoarthritic SDSCs and IL-1b and TNF-a in osteoarthritic synovial fluid. Phenotypic characterization of whole cells and CD14-negative cells showed no significant differences in levels of stem cell markers. mRNA expression of type II collagen was higher in CD14-negative cell pellets than in whole cell pellets. Immunohistochemical staining indicated higher levels of type II collagen in the CD14-negative cell pellets of CD14 high cells than in whole cell pellets of CD14 high cells. As expected, IL-1b and TNF-a significantly inhibited the expression of chondrogenic-related genes in SDSCs, an effect which was antagonized by knockdown of NF-kB and C/EBPb. Our results suggest that depletion of CD14 + synovial macrophages leads to improved chondrogenic potential in CD14 high cell populations in osteoarthritic SDSCs, and that NF-kB (RelA) and C/EBPb are critical factors mediating IL-1b-induced suppression of the chondrogenic potential of human SDSCs.

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An explorative study comparing levels of soluble mediators in control and osteoarthritic synovial fluid

Cited by 113 publications

References 11 publications

Women with Osteoarthritis have Elevated Synovial Fluid Levels of Insulin-Like Growth Factor (IGF)-1 and IGF-Binding Protein-3

Women with Osteoarthritis have Elevated Synovial Fluid Levels of Insulin-Like Growth Factor (IGF)-1 and IGF-Binding Protein-3

The Chemokine CX3CL1 (Fractalkine) and its Receptor CX3CR1: Occurrence and Potential Role in Osteoarthritis

Effects of CD14 Macrophages and Proinflammatory Cytokines on Chondrogenesis in Osteoarthritic Synovium-Derived Stem Cells

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