An Extract of Tagetes lucida and Its Phenolic Constituents as Antioxidants

Aquino, Rita Patrizia; Cáceres, Armando; Morelli, Silvana; Rastrelli, Luca

doi:10.1021/np020018i

Cited by 66 publications

(61 citation statements)

References 20 publications

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“…The antiradical activity of quercetin as positive control was determined using the stable 1,1'-diphenyl-2-picrylhydrazyl (DPPH) (12)(13)(14). The quenching of free radicals by A.punensis lyophilized EtOH:H 2 O extract was evaluated spectrophotometrically at 515 nm against the absorbance of the DPPH radical.…”

Section: Dpph Decoloration Assaymentioning

confidence: 99%

Antioxidant activity of 50% aqueous - ethanol extract from Acantholippia deserticola

et al. 2008

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The antioxidant properties of Acantholippia deserticola, a herb used in traditional northern Chilean medicine was studied using free radical -generating systems. The 50% aqueous -ethanol extract of Acantholippia deserticola protected against non-enzymatic lipid peroxidation in microsomal membranes of rat, induced by an Fe ++ -ascorbate system and measured spectrophotometrically by the TBARS test, and had strong free radical scavenging capacities on stable ABTS and DPPH radicals. The results shows that the IC 50 value of the 50% aqueous -ethanolic extract of A.deserticola is 18 ± 0.5 μg/ mL in DPPH radical -scavenging, 15 ± 0.8 μg/mL in lipid peroxidation , Total Antioxidant Activity (TAA) is 0.95 mM of Trolox per mg/mL of extract. The total phenolics content of extract is 725 ± 12 mg of gallic acid equivalent per g of dried extract. The results indicate that the 50% aqueous -ethanol extract of Acantholippia deserticola clearly has antioxidant properties

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Section: Dpph Decoloration Assaymentioning

confidence: 99%

Antioxidant activity of 50% aqueous - ethanol extract from Acantholippia deserticola

et al. 2008

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“…1D and 2D NMR experiments were obtained using the conventional pulse sequences as previously described (Aquino et al 2002). Electrospray-ionization mass spectrometry (ESIMS) were recorded using a Finnigan LC-Q Deca instrument (Thermoquest, San Jose, CA, USA) equipped with Xcalibur software.…”

Section: Instrumentsmentioning

confidence: 99%

“…The minor constituents were identified as vitexin (4) (Picerno et al 2003), isovitexin (5) (Picerno et al 2003), gallic acid (6) (Aquino et al 2002) and epi-catechin (7) (Foo et al 1996).…”

Section: Fractionation Proceduresmentioning

confidence: 99%

“…Extract A, dissolved in methanol, was analysed for its total phenolic content according to the Folin-Ciocalteu colorimetric method (Aquino et al 2002). Total phenols were expressed as myricetin equivalents (387.97 AE 2.1 g/ mg extract).…”

Section: Quantitative Determination Of Total Phenolsmentioning

confidence: 99%

“…Compound 2 was the major constituent, representing 6.13% w/w of the extract, compound 1 was 3.04%, and compound 3 was 1.54% w/w of the extract as shown by HPLC analysis (see Methods section). The extract had a total phenolic content, determined by the colorimetric Folin-Ciocalteu method (Aquino et al 2002) and expressed as myricetin equivalents, equal to 387.97 AE 2.1 (g/mg extract). Minor constituents were identified as vitexin (4) (Picerno et al 2003), isovitexin (5) (Picerno et al 2003), gallic acid (6) (Aquino et al 2002) and epicatechin (7) (Foo et al 1996), by ESIMS and NMR spectroscopic methods and by comparison with data reported in the literature.…”

Section: Phytochemical Analysismentioning

confidence: 99%

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An extract of Lannea microcarpa: composition, activity and evaluation of cutaneous irritation in cell cultures and reconstituted human epidermis

Picerno

Mencherini

Loggia

et al. 2006

Journal of Pharmacy and Pharmacology

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Lannea microcarpa (Anacardiaceae) is a tropical tree used in African folk medicine and commercial dermopharmaceutical formulations. Fractionation and analysis of its polar extract allowed the identification of 4, gallic acid and epi-catechin, as the major constituents. In-vivo assay (the croton oil ear test in mice) showed that the extract had significant anti-inflammatory effect (ID50 ¼ 900 g cm À2) but ten times lower than that of indometacin (ID50 ¼ 93 g cm À2 ), the non-steroidal anti-inflammatory drug used as reference. Cytotoxicity and cutaneous irritation of the extract and its constituents were investigated. The crude extract and its major components did not affect cell viability in-vitro either in three different cultures (J774.A1, WEHI-164 and HEK-293) of cells grown in monolayers or in the reconstituted human epidermis (RHE, 3D model), nor did they cause release of pro-inflammatory mediators (IL-1) or histomorphological modification of RHE.

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SU‐8/Pyrex microchip electrophoresis with integrated electrochemical detection for class‐selective electrochemical index determination of phenolic compounds in complex samples

et al. 2013

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A SU-8/Pyrex single-channel microchip integrating three 100 μm Pt electrodes (MCE-ED) for class-selective electrochemical index determination (CSEID) of phenolic acids and flavonoids in complex extracts of Tagetes lucida (Tl), Mentha piperita (Mp), Cymbopogon citratus (Cc), Calendula officinalis (Co), and Cynara scolymus (Cs) is proposed. Under strategic conditions controlled by a MES buffer (pH 5.0; 25 mM) and accordingly to the antioxidant acid-base properties, the simultaneous measurement of total acids and flavonoids indexes was achieved in less than 100 s with excellent analytical performance. The reliability of MCE-ED approach was demonstrated toward the high agreement between the total phenolic content obtained using microchip approach with those obtained by the well-established HPLC-DAD; revealing both identical order regarding the total phenolic content in the target samples. In addition, further comparison of MCE-ED with the traditional Folin-Ciocalteu antioxidant capacity assay, showed that MCE-ED approach could become a class-selective antioxidant capacity assay revealing that the sample antioxidant capacity was decreasing as Tl > Mp > Cs > Cc > Co according to their endogenous polyphenol content. These results suggested that the microchip approach is not only a reliable method for fast assessment of class-selective antioxidants constituting a very good alternative to the long analysis times and the using of toxic solvents required in HPLC but a novel truly antioxidant capacity assay. This excellent analytical performance is connected with the key-features of the "ready-to-use" system employed in this work such as portability, full integration of electrochemical detection, easy-operation, and potential MCE-ED disposability.

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An Extract of Tagetes lucida and Its Phenolic Constituents as Antioxidants

Cited by 66 publications

References 20 publications

Antioxidant activity of 50% aqueous - ethanol extract from Acantholippia deserticola

Antioxidant activity of 50% aqueous - ethanol extract from Acantholippia deserticola

An extract of Lannea microcarpa: composition, activity and evaluation of cutaneous irritation in cell cultures and reconstituted human epidermis

SU‐8/Pyrex microchip electrophoresis with integrated electrochemical detection for class‐selective electrochemical index determination of phenolic compounds in complex samples

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