An HPLC method for the pharmacokinetic study of vincristine sulfate-loaded PLGA–PEG nanoparticle formulations after injection to rats

Chen, Jianian; He, Huijuan; Li, Shaoshun; Shen, Qi

doi:10.1016/j.jchromb.2011.05.031

Cited by 27 publications

(9 citation statements)

References 23 publications

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“…The PSZ and VCR retention times using our simultaneous assay lie within the reported retention times for PSZ and VCR, respectively [ 13 – 18 ]. There is a slight interference from an endogenous plasma peak in the PSZ peak; this interference resulted in the fact that we could not quantify the PSZ peak accurately below the 50 ng/mL concentration; however, as mentioned before, it did not interfere with any of the validation parameters within our concentration range 50–5000 ng/mL.…”

Section: Discussionsupporting

confidence: 79%

“…Different chromatographic methods have been described for this task such as micellar electrokinetic chromatography (MEKC) [ 9 ] and several liquid chromatographic methods coupled with tandem mass spectrometry [ 10 , 11 ], fluorescence detection [ 12 ], and UV detection [ 13 – 16 ]. On the other hand, estimation of VCR in plasma samples has been carried out using HPLC-UV detection [ 17 , 18 ], UPLC-MS-MS [ 19 ], and several HPLC-MS-MS methods [ 20 – 23 ]. Besides, an HPLC-MS-MS method was presented for the simultaneous determination of VCR and actinomycin-D in human dried blood spots [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

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High Performance Liquid Chromatographic Assay for the Simultaneous Determination of Posaconazole and Vincristine in Rat Plasma

Khalil

El-Yazbi

Belal

et al. 2015

International Journal of Analytical Chemistry

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Purpose. Developing a validated HPLC-DAD method for simultaneous determination of posaconazole (PSZ) and vincristine (VCR) in rat plasma. Methods. PSZ, VCR, and itraconazole (ITZ) were extracted from 200 μL plasma using diethyl ether in the presence of 0.1 M sodium hydroxide solution. The organic layer was evaporated in vacuo and dried residue was reconstituted and injected through HC-C18 (4.6 × 250 mm, 5 μm) column. In the mobile phase, acetonitrile and 0.015 M potassium dihydrogen orthophosphate (30 : 70 to 80 : 20, linear gradient over 7 minutes) pumped at 1.5 mL/min. VCR and PSZ were measured at 220 and 262 nm, respectively. Two Sprague Dawley rats were orally dosed PSZ followed by iv dosing of VCR and serial blood sampling was performed. Results. VCR, PSZ, and ITZ were successfully separated within 11 min. Calibration curves were linear over the range of 50–5000 ng/mL for both drugs. The CV% and % error of the mean were ≤18% and limit of quantitation was 50 ng/mL for both drugs. Rat plasma concentrations of PSZ and VCR were simultaneously measured up to 72 h and their calculated pharmacokinetics parameters were comparable to the literature. Conclusion. The assay was validated as per ICH guidelines and is appropriate for pharmacokinetics drug-drug interaction studies.

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Section: Discussionsupporting

confidence: 79%

Section: Introductionmentioning

confidence: 99%

High Performance Liquid Chromatographic Assay for the Simultaneous Determination of Posaconazole and Vincristine in Rat Plasma

Khalil

El-Yazbi

Belal

et al. 2015

International Journal of Analytical Chemistry

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“…In Figure 10 , we found that the plasma concentration of the control group (DTX suspension) decreased sharply after the peak of concentration while that of nanoparticles decreased gradually. The reason for the slow rate of nanoparticles after administration was that the drug was not easily released from the nanoparticles [ 47 ]. DTX inside PLGA nanoparticles may possibly perforate the blood vessel wall and then enter into other tissues or organs.…”

Section: Resultsmentioning

confidence: 99%

Folate and Borneol Modified Bifunctional Nanoparticles for Enhanced Oral Absorption

et al. 2018

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Oral delivery is considered the preferred route of administration due to its convenience and favorable compliance. Here, docetaxel (DTX) loaded polylactic-co-glycolic acid (PLGA) nanoparticles, coated with polyethyleneimine–folic acid (PEI-FA) and polyethyleneimine–borneol (PEI-BO), were designed to enhance oral absorption (FA/BO-PLGA-NPs). The FA/BO-PLGA-NPs were spherical and smooth with an average size of (137.0 ± 2.1) nm. Encapsulation efficiency (EE%) and drug loading (DL%) were (80.3 ± 1.8)% and (2.3 ± 0.3)%, respectively. In vitro release studies showed that approximately 62.1% of DTX was released from FA/BO-PLGA-NPs in media at pH 7.4. The reverted gut sac method showed that the absorption of FA/BO-PLGA-NPs in the intestines was approximately 6.0 times that of DTX. Moreover, cellular uptake suggested that the obtained FA/BO-PLGA-NPs could be efficiently internalized into Caco-2 cells via FA-mediated active targeting and BO-mediated P-glycoprotein (P-gp) inhibition. Pharmacokinetics study demonstrated that after oral administration of DTX at a dose of 10 mg/kg in FA/BO-PLGA-NPs, the bioavailability of FA/BO-PLGA-NPs was enhanced by approximately 6.8-fold compared with that of DTX suspension. FA/BO-PLGA-NPs caused no obvious irritation to the intestines. Overall, the FA/BO-PLGA-NP formulation remarkably improved the oral bioavailability of DTX and exhibited a promising perspective in oral drug delivery.

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“…The rats in the control groups were injected with native VS solution and VSLI (1.2 mg of VS/kg, 0.6 mL/kg) intravenously into the tail vein. 40 The other three groups (the HSPC formulation, PEG-DSPE formulation, and PEG-PLGA formulation) were injected into the tail vein with a dose of 0.6 mL/kg and a concentration of 2.0 mg/mL. For the 1.0 mg/mL chitosan formulation, the treated rats were injected at 1.2 mL/kg.…”

Section: Pharmacokinetics Studiesmentioning

confidence: 99%

The influence of different long-circulating materials on the pharmacokinetics of liposomal vincristine sulfate

Zhang

Chen

Li³

et al. 2016

IJN

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Purpose This study was designed to improve the in vivo pharmacokinetics of long-circulating vincristine sulfate (VS)-loaded liposomes; three different long-circulating materials, chitosan, poly(ethylene glycol)-1,2-distearoyl sn-glycero-3-phosphatidylethanolamine (PEG-DSPE), and poly(ethylene glycol)-poly-lactide- co -glycolide (PEG-PLGA), were evaluated at the same coating molar ratio with the commercial product Marqibo ® (vincristine sulfate liposome injection [VSLI]). Materials and methods VS-loaded liposomes were prepared by a pH gradient method and were then coated with chitosan, PEG-DSPE, or PEG-PLGA. Physicochemical properties, including the morphology, particle size, zeta potential, encapsulation efficiency (EE%), pH, drug loading, and in vitro release, were determined. Preservation stability and pharmacokinetic studies were performed to compare the membrane-coated liposomes with either commercially available liposomes or the VS solution. Results The sphere-like morphology of the vesicles was confirmed by transmission electron microscope. Increased particle size, especially for the chitosan formulation, was observed after the coating process. However, the EE% was ~99.0% with drug loading at 2.0 mg/mL, which did not change after the coating process. The coating of long-circulation materials, except for chitosan, resulted in negatively charged and stable vesicles at physiological pH. The near-zero zeta potential exhibited by the PEG-DSPE formulation leads to a longer circulation lifetime and improved absorption for VS, when compared with the PEG-PLGA formulation. Compared with the commercial product, PEG was responsible for a higher plasma VS concentration and a longer half-life. Conclusion PEG-DSPE coating may be related to better absorption, based on the stability and a pharmacokinetic improvement in the blood circulation time.

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An HPLC method for the pharmacokinetic study of vincristine sulfate-loaded PLGA–PEG nanoparticle formulations after injection to rats

Cited by 27 publications

References 23 publications

High Performance Liquid Chromatographic Assay for the Simultaneous Determination of Posaconazole and Vincristine in Rat Plasma

High Performance Liquid Chromatographic Assay for the Simultaneous Determination of Posaconazole and Vincristine in Rat Plasma

Folate and Borneol Modified Bifunctional Nanoparticles for Enhanced Oral Absorption

The influence of different long-circulating materials on the pharmacokinetics of liposomal vincristine sulfate

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