An <i>Arabidopsis</i> (malectin‐like) leucine‐rich repeat receptor‐like kinase contributes to downy mildew disease

Hok, Sophie; Danchin, Etienne G. J.; Allasia, Valérie; Panabières, Franck; Attard, Agnès; Keller, Harald

doi:10.1111/j.1365-3040.2011.02390.x

Cited by 104 publications

(137 citation statements)

References 77 publications

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“…This putative extracellular, C-terminus domain is likely involved in carbohydrate binding of maltoselike glucose oligomers (Schallus et al, 2008). A similar Malectin-like LRR-RLK gene was found to contribute to downy mildew disease in Arabidopsis (Hok et al, 2011). Furthermore, we observed evidence of various functional domains fusions, predominantly at the C-termini of M. notabilis LRR-RSTKs, mostly integrated by the Pkinase_Tyr and the Malectin or Malectin-like domains.…”

Section: Discussionsupporting

confidence: 58%

In silico analysis of the LRR receptor-like serine threonine kinases subfamily in Morus notabilis

Zambounis¹,

Psomopoulos²,

Ganopoulos³

et al. 2016

Plant Omics

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Mulberries are important trees crops for orchards and agroforestry systems, which are plagued by many phytopathogenic fungal species. Leucine rich repeats (LRRs) receptor-like serine threonine kinases (LRR-RSTK) subfamily plays an important role in plant defense-related reactions against fungal attacks. In the present study, we mined this subfamily on Morus notabilis, a mulberry species whose relevant annotated genome assembly has recently become publicly available. Our aim was to decipher in silico the expansion and phylogeny of these genes, their homology relationships against their orthologous in woody angiosperm plant species, and the existence of positive selective pressures acting upon their LRRs. This subfamily was found to be quite abundant and diverged, comprising by 142 annotated gene members and containing a range of conserved functional domains in their C-termini, whilst their LRRs number ranged from one to 17 repeats. A phylogenetic investigation revealed 12 distinct clades based on their diverse structural profiles, mainly as a result of the fused functional domains at their C-termini. The interspecific expansion of these M. notabilis LRR-RSTKs has been investigated by a homology analysis across 12 other woody angiosperm species, showing that the highest proportion of homologous best BLAST hits observed primarily in Prunus persica, Malus domestica and Theobroma cacao. Using a series of maximum likelihood analyses, extensive episodes of positive selective pressures acting across the LRRs were observed. This overall evidence supports a potential crucial role of this diverged LRR-RSTK subfamily as a surveillance mechanism of M. notabilis against fungal attacks by providing rapidly evolving ligand-binding specificities.

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Section: Discussionsupporting

confidence: 58%

In silico analysis of the LRR receptor-like serine threonine kinases subfamily in Morus notabilis

Zambounis¹,

Psomopoulos²,

Ganopoulos³

et al. 2016

Plant Omics

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“…We previously identified the RLK IMPAIRED OOMYCETE SUSCEPTIBILITY1 (IOS1; At1g51800) as required for the full susceptibility of Arabidopsis (Arabidopsis thaliana) to the downy mildew pathogen Hyaloperonospora arabidopsidis (Hpa; Hok et al, 2011). IOS1 belongs to a subfamily of approximately 50 RLKs in Arabidopsis, which possess an extracellular region composed of a malectin-like domain (MLD) in addition to (two to three) leucine-rich repeats (LRRs; Hok et al, 2011).…”

mentioning

confidence: 99%

“…IOS1 belongs to a subfamily of approximately 50 RLKs in Arabidopsis, which possess an extracellular region composed of a malectin-like domain (MLD) in addition to (two to three) leucine-rich repeats (LRRs; Hok et al, 2011). Malectin was first identified in Xenopus laevis as an endoplasmic reticulum-localized carbohydrate-binding protein (Schallus et al, 2008) that controls the N-glycosylation status of secreted glycoproteins .…”

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confidence: 99%

The Receptor Kinase IMPAIRED OOMYCETE SUSCEPTIBILITY1 Attenuates Abscisic Acid Responses in Arabidopsis

et al. 2014

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(Al.A., L.M.V.) In plants, membrane-bound receptor kinases are essential for developmental processes, immune responses to pathogens and the establishment of symbiosis. We previously identified the Arabidopsis (Arabidopsis thaliana) receptor kinase IMPAIRED OOMYCETE SUSCEPTIBILITY1 (IOS1) as required for successful infection with the downy mildew pathogen Hyaloperonospora arabidopsidis. We report here that IOS1 is also required for full susceptibility of Arabidopsis to unrelated (hemi)biotrophic filamentous oomycete and fungal pathogens. Impaired susceptibility in the absence of IOS1 appeared to be independent of plant defense mechanism. Instead, we found that ios1-1 plants were hypersensitive to the plant hormone abscisic acid (ABA), displaying enhanced ABA-mediated inhibition of seed germination, root elongation, and stomatal opening. These findings suggest that IOS1 negatively regulates ABA signaling in Arabidopsis. The expression of ABA-sensitive COLD REGULATED and RESISTANCE TO DESICCATION genes was diminished in Arabidopsis during infection. This effect on ABA signaling was alleviated in the ios1-1 mutant background. Accordingly, ABA-insensitive and ABA-hypersensitive mutants were more susceptible and resistant to oomycete infection, respectively, showing that the intensity of ABA signaling affects the outcome of downy mildew disease. Taken together, our findings suggest that filamentous (hemi)biotrophs attenuate ABA signaling in Arabidopsis during the infection process and that IOS1 participates in this pathogen-mediated reprogramming of the host.

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“…In tomato, most cloned resistance genes also belong to this family, including Prf, I2, Mi-1, Mi-9, Sw-5/Mi, and Hero (Salmeron et al, 1996;Ori et al, 1997;Brommonschenkel et al, 2000;Ernst et al, 2002;Tameling et al, 2002;Jablonska et al, 2007). The receptor-like protein kinase is also considered as a disease resistance gene family, and these genes have been shown to be involved in plant defense against pathogens (Kruijt et al, 2005;Hok et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

In silico analysis of gene content in tomato genomic regions mapped to the Ty-2 resistance gene

et al. 2015

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ABSTRACT. Tomato yellow leaf curl virus is one of the main diseases affecting tomato production worldwide. Previous studies have shown that Ty-2 is an important resistance gene located between molecular markers C2_At2g28250 (82.3 cM) and T0302 (89.0 cM), and exhibits strong resistance to tomato yellow leaf curl virus in Asia. In this study, Ty-2 candidate genes were subjected to bioinformatic analysis for the sequenced tomato genome. We identified 69 genes between molecular markers C2_At2g28250 and T0302, 22 of which were disease-related resistant genes, including nucleotide binding site-leucine-rich repeat disease resistance genes, protease genes (protein kinase, kinase receptor, and protein isomerase), cytochromes, and transcription factors. Expressed sequence tag analysis revealed that 77.3% (17/22) of candidate disease-resistance genes were expressed, involving 143 expressed sequence tags. Based on full-length cDNA sequence analysis, 7 candidate genes were found, 4 of which were involved in tomato responses to pathogens. Microarray ex-Y.F. Liu et al. 7948©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (3): 7947-7956 (2015) pression analysis also showed that most candidate genes were involved in the tomato responses to multiple pathogens, including fungi, viruses, and bacteria. RNA-seq expression analysis revealed that all candidate genes participated in tomato growth and development.

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An Arabidopsis (malectin‐like) leucine‐rich repeat receptor‐like kinase contributes to downy mildew disease

Cited by 104 publications

References 77 publications

In silico analysis of the LRR receptor-like serine threonine kinases subfamily in Morus notabilis

In silico analysis of the LRR receptor-like serine threonine kinases subfamily in Morus notabilis

The Receptor Kinase IMPAIRED OOMYCETE SUSCEPTIBILITY1 Attenuates Abscisic Acid Responses in Arabidopsis

In silico analysis of gene content in tomato genomic regions mapped to the Ty-2 resistance gene

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