An<i>In Vitro</i>Investigation of Platelet-Rich Plasma-Gel as a Cell and Growth Factor Delivery Vehicle for Tissue Engineering

Jalowiec, Jagoda M; D’Este, Matteo; Bara, Jennifer J.; Denom, Jessica; Menzel, Ursula; Alini, Mauro; Verrier, Sophie; Herrmann, Marietta

doi:10.1089/ten.tec.2015.0223

Cited by 63 publications

(59 citation statements)

References 57 publications

(62 reference statements)

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“…Next to the fact that all platelet-derived products are subject of large donor variation [3], this represents a major hurdle in comparing different studies and ultimately drawing conclusions on the efficacy of PRP in these approaches. This is also highlighted in a recent study systematically investigating the influence of different platelet concentrations on both the release of growth factors and cells incorporated in PRP gels [8]. This work demonstrated that the concentration of released growth factors is strongly correlated with the platelet concentration.…”

Section: Prp In Tissue-engineered Constructsmentioning

confidence: 59%

“…Upon activation, PRP forms a soft hydrogel as determined in a recent rheology study [8]. As such, it represents a suitable material for soft tissue repair.…”

Section: Prp In Tissue-engineered Constructsmentioning

confidence: 92%

“…This work demonstrated that the concentration of released growth factors is strongly correlated with the platelet concentration. Moreover, it was shown that the platelet concentration in PRP gels has an influence on incorporated MSCs, with most favorable conditions in terms of cell viability and proliferation observed for PRP gels with a platelet concentration of 1,000 × 10 3 platelets/µL (referring to a 3.3-to 6.7-fold platelet enrichment based on a platelet concentration ranging from 150-300 × 10 3 platelets/µL in healthy human donors) [8]. PRP has been used most often in the field of bone tissue engineering, where PRP has been applied as a cell carrier and/or as a source of osteogenic and angiogenic factors.…”

Section: Prp In Tissue-engineered Constructsmentioning

confidence: 99%

“…Most commonly, thrombin and/or calcium chloride are used for this purpose [7]. Once activated, PRP will eventually form a hydrogel, which has been reported to secret growth factors [8] and may be applied as delivery vehicle for cells [8,9], as is discussed below.…”

Section: Introductionmentioning

confidence: 99%

“…Exosomes might therefore play an important role in the effect of PL and represent a delivery system for cell-free therapy strategies [11]. Jalowiec et al [8] showed that there is a positive correlation between the platelet concentration in thrombin activated platelet gel and the protein secretion and observed the highest release of growth factors within the first 4 h after gelification. High levels of TGF-β 1 could be demonstrated for all platelet concentrations, followed by PDGF-AB and PDGF-BB.…”

Section: Introductionmentioning

confidence: 99%

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Platelet-Rich Plasma in Tissue Engineering: Hype and Hope

Lang

Loibl²,

Herrmann³

2018

Eur Surg Res

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Background: Platelet-rich plasma (PRP) refers to an enriched platelet suspension in plasma. In addition to the clinical application of PRP in the context of various orthopedic diseases and beyond, PRP and platelet lysate (PL) have been in focus in the field of tissue engineering. In this review, we discuss the application of PRP as a cell culture supplement and as part of tissue engineering strategies, particularly emphasizing current hurdles and ambiguities regarding the efficacy of PRP in these approaches. Summary: As a putative autologous replacement for animal-derived supplements such as fetal calf serum (FCS), PRP has been applied as cell culture supplement for the expansion of stem and progenitor cells for tissue engineering applications and cell therapies. Attributed to the high content of growth factors in platelets, PRP has been shown to promote cell growth, which was mostly superior to standard cultures supplemented with FCS, while the differentiation capacity of progenitor cells seems not to be affected. However, it was also suggested that cultivation of cells with PRP significantly alters the protein expression profile in cells in comparison to FCS, indicating that the influence of PRP on cell behavior should be thoroughly investigated. Moreover, different PRP preparation methods and donor variations have to be considered for the use of PRP under good manufacturing practice conditions. PRP has been used for various tissue engineering applications in the context of bone, cartilage, skin, and soft tissue repair, where most studies were conducted in the field of bone tissue engineering. These approaches take either advantage of the release of chemoattractive, angiogenic, proliferative, and putatively pro-regenerative growth factors from PRP, and/or the hydrogel properties of activated PRP, making it suitable as a cell delivery vehicle. In many of these studies, PRP is combined with biomaterials, cells, and in some cases recombinant growth factors. Although the experimental design often does not allow conclusions on the pro-regenerative effect of PRP itself, most publications report beneficial effects if PRP is added to the tissue-engineered construct. Furthermore, it was demonstrated that the release of growth factors from PRP may be tailored and controlled when PRP is combined with materials able to capture growth factors. Key Messages: Platelet-derived preparations such as PRP and PL represent a promising source of autologous growth factors, which may be applied as cell culture supplement or to promote regeneration in tissue-engineered constructs. Furthermore, activated PRP is a promising candidate as an autologous cell carrier. However, the studies investigating PRP in these contexts often show conflicting results, which most likely can be attributed to the lack of standardized preparation methods, particularly with regard to the platelet content and donor variation of PRP. Ultimately, the use of PRP has to be tailored for the individual application.

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Section: Prp In Tissue-engineered Constructsmentioning

confidence: 59%

“…Upon activation, PRP forms a soft hydrogel as determined in a recent rheology study [8]. As such, it represents a suitable material for soft tissue repair.…”

Section: Prp In Tissue-engineered Constructsmentioning

confidence: 92%

Section: Prp In Tissue-engineered Constructsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Platelet-Rich Plasma in Tissue Engineering: Hype and Hope

Lang

Loibl²,

Herrmann³

2018

Eur Surg Res

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Long‐Term Recruitment of Endogenous M2 Macrophages by Platelet Lysate‐Rich Plasma Macroporous Hydrogel Scaffold for Articular Cartilage Defect Repair

Pan

Yuan

Xun

et al. 2022

Adv Healthcare Materials

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After cartilage damage, a large number of monocytes/macrophages infiltrate into adjacent synovium and the resident macrophages in synovial tissue transform to activated macrophages (M1), which secrete pro-inflammatory cytokines to induce sustained inflammation and chondrocyte apoptotic. However, current clinical therapies for cartilage repair can rarely achieve long-term anti-inflammatory regulation and satisfactory outcomes. Herein, a platelet lysate-rich plasma macroporous hydrogel (PLPMH) scaffold with around 100 μm pore size and 1.25 MPa Young's modulus is developed to sustainedly recruit and polarize endogenous anti-inflammatory macrophages (M2) for improving cartilage defect repair. PLPMH scaffold can steadily release sphingosine1-phosphate and proteins via gradual degradation, thus inducing M2 macrophages migration or resting (M0) macrophages migration and then polarization to M2 phenotype, and improving the secretion of anti-inflammatory cytokines. Furthermore, PLPMH scaffold exhibits negligible inflammatory responses in vivo and promotes endogenous M2 macrophage infiltration in large numbers and long-time duration to provide a local anti-inflammatory microenvironment, which even lasts for 42 d. In a rabbit model of cartilage defect, PLPMH scaffold increases the ratio of M2 macrophages and improves cartilage tissue regeneration. These studies support that PLPMH scaffold may have a great potential in articular cartilage tissue engineering by providing an anti-inflammatory and pro-regenerative microenvironment.

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Platelet-rich plasma-based bioactive membrane as a new advanced wound care tool

Spanò

Muraglia

Todeschi

et al. 2017

J Tissue Eng Regen Med

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Chronic skin ulcers, consequence of diabetes and other pathological conditions, heavily compromise the patient life quality and represent a high and constantly growing cost for National Health Services. Autologous platelet-rich plasma (PRP), has been proposed to treat these lesions. The absence of guidelines for the PRP production and the need of a fresh preparation for each treatment lead us to develop a protocol for the production of an allogenic PRP-based bioactive membrane (BAM), standardized for platelet concentration and growth factor release. This work compares BAMs obtained starting from two different platelet concentrations. There was no direct correlation between the amount of growth factors released by BAM in vitro and the initial platelet count. However, different release kinetics were noticed for different growth factors, suggesting that they were differently retained by the two BAMs. The angiogenic potential of both BAMs was determined by Luminex Angiogenesis Assay. The biological activity of the factors released by the two BAMs was confirmed by cell proliferation and migration. A diabetic mouse chronic ulcer model was used to define the best PRP therapeutic dose in vivo. Both BAMs induced wound healing by increasing the thickness of the regenerated epidermis and the vessel number. However, a too high platelet concentration resulted in a slowdown of the membrane resorption that interfered with the skin healing. Overall, the results indicate that the BAMs could represent a natural and effective wound healing tool for the treatment of skin ulcers. Copyright © 2016 John Wiley & Sons, Ltd.

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AnIn VitroInvestigation of Platelet-Rich Plasma-Gel as a Cell and Growth Factor Delivery Vehicle for Tissue Engineering

Cited by 63 publications

References 57 publications

Platelet-Rich Plasma in Tissue Engineering: Hype and Hope

Platelet-Rich Plasma in Tissue Engineering: Hype and Hope

Long‐Term Recruitment of Endogenous M2 Macrophages by Platelet Lysate‐Rich Plasma Macroporous Hydrogel Scaffold for Articular Cartilage Defect Repair

Platelet-rich plasma-based bioactive membrane as a new advanced wound care tool

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