Anin vitromicrobial-based model for studying caries-preventive agents

Abstract

In conclusion, our in vitro system is suitable for testing potential caries-preventive agents in a reproducible way by using whole tooth samples and offers full access together with the possibility of manipulating the specimens during operation.

Effect of microparticulate silver addition in dental adhesives on secondary caries in vitro

“…Experiences with the cariogenity of the selected kind of S. mutans have been reported previously [9,13,33,46]. As a consequence, in the chosen set up, distinct demineralization was evident already after short incubation times.…”

“…Due to ethical problems with experimental clinical studies, in vitro models have the potential to elucidate fundamental aspects of caries processes by simulating the human oral microcosm [12,[23][24][25][26][27][28][29][30][31][32][33]. Main advantage here is that artificially proliferated plaque may be incubated with particularly interesting bacteria [14,[32][33][34].…”

“…Main advantage here is that artificially proliferated plaque may be incubated with particularly interesting bacteria [14,[32][33][34]. Plaque and saliva of human subjects, however, exhibit individual spectra negatively influencing data scatter [12,28,33,35].…”

“…Plaque and saliva of human subjects, however, exhibit individual spectra negatively influencing data scatter [12,28,33,35]. Fundamental requirements for an effective artificial mouth are chambers with temperature remote, simulation of intraoral sugar clearance by addressing the average saliva flow rate, and a nutrition medium being exactly adjusted to the used bacteria [12,26,33,35].…”

Glycan-binding specificities of Streptococcus mutans and Streptococcus sobrinus lectin-like adhesins

“…Although the lectin-mediated binding specificities of caries-inducing microorganisms are not very well characterized yet, studies revealed that a high molecular mass cranberry constituent was capable of reducing mutans streptococci level in saliva and effectively inhibiting in vitro adhesion to hydroxyapatite [22,23]. Furthermore, studies in an artificial mouth model showed a caries-preventive effect of an experimental glycan solution prepared by hydrolysis of porcine gastric mucine on natural teeth [24]. Early adhesion experiments by Gibbons and Qureshi revealed that the adhesion of different S. mutans serotypes to saliva-coated hydroxyapatite could be inhibited by galactose and melibiose (6-α-D-galactosyl-glucose) but not by lactose [25].…”