Anin vivomethod for measuring the adsorption of plasma proteins to titanium in humans

Abstract

A novel method of collecting in vivo plasma proteins of humans from osteotomies prepared during insertion of an oral implant is described. A rod containing a collecting portion with a predetermined surface is introduced into the osteomy, removed, and transferred for enzyme-linked immunosorbent assay analysis. Two experiments were used to examine the feasibility of the method. In the first, titanium (Ti) rods with different roughness were exposed for 10 min to the blood. Blasted and acid-etched surfaces adsorbed four times more and acid-etched surfaces adosorbed two times more plasma proteins as compared to machined surfaces. In the second experiment, blasted and acid-etched rods were wetted for 10 s prior to the insertion. The adsorption for fibronectin, albumin, fibrinogen, and IgG was enhanced significantly compared with nonwetted rods. These results are discussed in the light of previous methods used in studies on adsorption. Thus, use of the collecting instrument enables aspects of human plasma-implant interface to be studied in a more realistic manner.

Intrasurgical Protein Layer on Titanium Arthroplasty Explants: From the Big Twelve to the Implant Proteome

“…However, the higher protein adsorption rate onto Plasmapore‐coated Ti surface compared to smoother surface geometries in our study correspond to the study of Kohavi et al . In their experiments they found four times higher protein adsorption for sand‐blasted Ti surfaces compared to machined surfaces.…”

“…Practically nothing is known about the biological effects and interactions between the other adsorbed proteins orchestrating implant integration in cementless total hip replacement. This is in contrast to other experimental studies emphasizing the relevance of other proteins such as fibronectin (Fn), fibrinogen or thrombin as a molecular glue, anchoring osteoblasts to the implant . Some in vitro data indicate that fibronectin showed excellent adsorption on titanium surfaces even at low concentrations in vitro .…”

Time course of surface characteristics of alkali‐ and heat‐treated titanium dental implants during vacuum storage

“…Nagassa et al observed that the degree of BSA adsorption in vitro on a surface with nanometer‐scale structural features was higher than that on a similar but smooth surface . Kohavi et al also observed that the in vivo adsorption of human plasma proteins on an acid‐etched rough surface (Ra = approximately 200 nm) was higher than that on a machined smooth surface (Ra = approximately 20 nm) . After the AH treatment, nanometer‐ and/or micrometer‐scale structural features were formed on the surface of the treated Ti sample, as shown in Figures and .…”

“…29 Kohavi et al also observed that the in vivo adsorption of human plasma proteins on an acidetched rough surface (Ra 5 approximately 200 nm) was higher than that on a machined smooth surface (Ra 5 approximately 20 nm). 30 After the AH treatment, nanometer-and/or micrometer-scale structural features were formed on the surface of the treated Ti sample, as shown in Figures 1 and 2. However, the original microscaled surface outline remained intact [ Figure 1(A)], and the surface roughness of the AHtreated was only slightly higher, as shown in Figure 2(A).…”

Susceptibility to corrosion of laser welding composite arch wire in artificial saliva of salivary amylase and pancreatic amylase

“…[41] α-Amylase formed clusters or aggregates when adsorbed onto the surface of interlayer like IgG and fibrinogen, resulting in differences in layer thickness and morphology. [42] (Fig. 3 and 4).…”