An IgM specific ELISA for the serodiagnosis of viral bovine respiratory infections

Florent, G.; Wiseman, Anat

doi:10.1016/0147-9571(90)90089-c

Cited by 9 publications

(14 citation statements)

References 6 publications

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“…The cause of the failure of the ELISA test in demonstrating sero-conversion following an experimental infection may be due to the use of class-specific (anti-IgG) conjugates, which are incapable of detecting class IgM antibodies. In this experiment, IgM class antibodies were detected 8 to 10 days post-infection (PI), while IgG class antibodies were detected 13 to 17 days PI [33]. Similar results were obtained in the experiment reported here, where IgG class antibodies were detected 10 to 16 days PI.…”

Section: Discussionsupporting

confidence: 80%

The Development and Application of an Indirect ELISA Test for the Detection of Chicken Anaemia Virus (CAV) by VP1 in Chicken Flock Serum

Mahgoub¹

2014

OJGen

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Chicken anaemia virus (CAV) causes a viral disease in chickens worldwide and thus has economic importance. The main aim of this study was to develop a rapid, sensitive and specific VP1-CAVI indirect ELISA for the detection of CAV infection. The CAV-VP1, was separately cloned and expressed in recombinant E. coli. The purified recombinant CAV-VP1 protein was then coated as an antigen on an ELISA plates to evaluate its reactivity against chicken sera. The resulting indirect ELISA was then compared with a commercial ELISA. The specificity and sensitivity of the indirect ELISA were measured as 93.3% and 100%, respectively. A t-test produced a t-value of 15.805 for the indirect ELISA and revealed a significant difference between CAV-positive serum and CAV-negative serum (p-value of 0.001). For the second variable (i.e., a commercial ELISA), the t-test yielded a t-value of 5.063, which revealed a significant difference between CAV-positive serum and CAV-negative serum (p-value of 0.015). This intervention produces statistically significant improvements in both variables (p-values < 0.05). The correlation coefficient for the indirect ELISA was r = 0.93. Therefore, this work can be considered as a new achievement in diagnosis for Chicken anaemia virus in chicken flocks.

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Section: Discussionsupporting

confidence: 80%

The Development and Application of an Indirect ELISA Test for the Detection of Chicken Anaemia Virus (CAV) by VP1 in Chicken Flock Serum

Mahgoub¹

2014

OJGen

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“…Similar results were obtained in another experiment where IgG1-class antibodies were detected 10 to 16 days PI. IgM-class antibodies were detected 10 days PI, they reached their peak on the 16th day PI and after that their level fell sharply until the 38th day after infetion when they were undetectable (Florent and Wiseman, 1990). In both experiments IgM and IgG1 class antibodies were detected at approximately the same time.…”

Section: Discussionmentioning

confidence: 59%

The development and application of an indirect Elisa test for the detection of antibodies to bovine respiratory syncytial virus in blood serum

Kovařčík¹

2001

Vet. Med. - Czech

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ABSTRACT:We developed an indirect enzyme-linked immunosorbent assay (ELISA) for detection of serum antibodies to bovine respiratory syncytial virus. For evaluation of the newly developed ELISA, field sera collected from 549 head of cattle in the Czech Republic were tested in parallel by a serum neutralization test. The tests showed 98.36% agreement. The specificity and sensitivity of the ELISA relative to serum neutralization test was 97.00% (226/233) and 99.37% (314/316), respectively. Tissue culture-grown viral antigen was used in the tests. The corrected optical density (COD) of each sample tested at dilution 1/100 was expressed as a percentage of the COD of a positive reference serum included on each plate, this value was the sample/positive (S/P) ratio. We determined the relationship between the S/P ratio (%) obtained at a dilution 1/100 and the end point titer calculated by serum neutralization test (r = 0.9743). The ELISA test was evaluated by testing acute and convalescent (3 wk later) serum pairs from 9 head of cattle with confirmed BRSV infection for demonstration of seroconversion. The ELISA test demonstrated a clear increase of the S/P ratio (%) between acute and convalescent serum pairs (on average 42.2 ± 13.1).Keywords: bovine respiratory syncytial virus (BRSV); respiratory tract pathogen; cattle; ELISA A list of abbreviations: BRSV bovine respiratory syncytial virus, SN test serum neutralization test, HIT haemagglutination inhibition test, FAT fluorescent antibody test, CPE cytopathic effect, OD optical density, PBS-T phosphate buffered saline with Tween 20, MEM minimal essential medium, RT-PCR reverse transcription-polymerase chain reaction.

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“…13 Testing of field sera confirmed the serodiagnostic potential of BHV-1 IgM, as in previous reports. 6,31 In this study, the detection of BHV-1 IgM by IIFAT was superior to testing by single-dilution ELISA for seroconversion (i.e., a change in status from seronegative to seropositive, or Ն4-fold increase in specific titer). This is in contrast to a previous report, 6 where, using a capture ELISA, BHV-1 IgM was detected less commonly than seroconversion on both an individual animal and herd basis.…”

Section: Discussionmentioning

confidence: 92%

“…Three conventionally reared calves (2,3,4), were each inoculated with 20 ml (10 8,43 TCID 50 /ml) of the Colorado strain of BHV-1 grown in fetal bovine lung cells (FBL), receiving 10 ml intratracheally and 5 ml in each nostril. Blood was collected from each calf 8 days before inoculation and 2, 3,6,8,10,13,15,17,20,23,27,30,34,36,38,41,43,45,48,55,61, and 68 days postinfection (dpi), and sera were stored at Ϫ20 C for subsequent testing. Each calf was reinfected in the same way at 30 dpi with an inoculum containing 10 6.65 TCID 50 /ml.…”

Section: Animals and Samplingmentioning

confidence: 99%

“…IgM-specific indirect and capture enzyme-linked immunosorbent assays (ELISAs) have been described for a number of bovine respiratory viruses, including bovine respiratory syncytial virus, 9,32 BHV-1, 6 and bo-vine coronavirus. 14 In addition to their use for making a diagnosis with a single acute serum sample, these assays have usually but not always been superior to the detection of seroconversion when used to test sera from field outbreaks of respiratory disease.…”

mentioning

confidence: 99%

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Evaluation of an Immunofluorescent Antibody Test to Detect Bovine Herpesvirus 1-Specific IgM

Graham¹,

Foster²,

German³

et al. 1999

J VET Diagn Invest

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Abstract. An indirect immunofluorescent antibody test (IIFAT) was developed to detect bovine herpesvirus 1 (BHV-1)-specific IgM. All sera were treated with protein-G agarose prior to testing to eliminate the possibility of false-positive results due to IgM-isotype rheumatoid factor (IgM-RF). Specific IgM was first detected 8 days after experimental infection of 3 calves free of maternally derived antibody, with peak responses occurring 2-7 days later. Seroconversion was detected in all 3 calves using a single-dilution enzyme-linked immunosorbent assay. Following reinfection at 30 days postinfection, a low-level IgM response was detected in only 1 calf. Seroconversion was detected in 2 calves. There was no evidence of activation of IgM-RF by infection or reinfection with BHV-1. When 87 acute and convalescent serum pairs collected from 21 outbreaks of respiratory disease were tested, specific IgM was detected in 58 animals (66.6%) from 19 (90.5%) outbreaks. Seroconversion was detected in 44 of these animals (50.6%) from 17 outbreaks (81.0%). The correlations between these 2 assays on a calf and outbreak basis were 79.3% and 90.5%, respectively. Specific IgM was detected in 17/ 20 sera (85.0%) collected from an additional outbreak. No virus was detected by virus isolation or immunofluorescent staining in nasal mucus samples collected at the same time. Detection of specific IgM by IIFAT is a useful technique for the serodiagnosis of BHV-1 infection.

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An IgM specific ELISA for the serodiagnosis of viral bovine respiratory infections

Cited by 9 publications

References 6 publications

The Development and Application of an Indirect ELISA Test for the Detection of Chicken Anaemia Virus (CAV) by VP1 in Chicken Flock Serum

The Development and Application of an Indirect ELISA Test for the Detection of Chicken Anaemia Virus (CAV) by VP1 in Chicken Flock Serum

The development and application of an indirect Elisa test for the detection of antibodies to bovine respiratory syncytial virus in blood serum

Evaluation of an Immunofluorescent Antibody Test to Detect Bovine Herpesvirus 1-Specific IgM

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