2023
DOI: 10.1021/cbmi.3c00102
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An Image Processing Algorithm for Facile and Reproducible Quantification of Vomocytosis

Neeraj Senthil,
Noah Pacifici,
Melissa Cruz-Acuña
et al.

Abstract: Vomocytosis is a process that occurs when internalized fungal pathogens escape from phagocytes without compromising the viability of the pathogen and the host cell. Manual quantification of time-lapse microscopy videos is currently used as the standard to study pathogen behavior and vomocytosis incidence. However, human-driven quantification of vomocytosis (and the closely related phenomenon, exocytosis) is incredibly burdensome, especially when a large volume of cells and interactions needs to be analyzed. In… Show more

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