Microorganism are ubiquitous and intimately connected
with human
health and disease management. The accurate and fast identification
of pathogenic microorganisms is especially important for diagnosing
infections. Herein, three tetraphenylethylene derivatives (S-TDs:
TBN, TPN, and TPI) featuring different cationic groups, charge numbers,
emission wavelengths, and hydrophobicities were successfully synthesized.
Benefiting from distinct cell wall binding properties, S-TDs were
collectively utilized to create a sensor array capable of imaging
various microorganisms through their characteristic fluorescent signatures.
Furthermore, the interaction mechanism between S-TDs and different
microorganisms was explored by calculating the binding energy between
S-TDs and cell membrane/wall constituents, including phospholipid
bilayer and peptidoglycan. Using a combination of the fluorescence
sensor array and a deep learning model of residual network (ResNet),
readily differentiation of Gram-negative bacteria (G−), Gram-positive
bacteria (G+), fungi, and their mixtures was achieved. Specifically,
by extensive training of two ResNet models with large quantities
of images data from 14 kinds of microorganism stained with S-TDs,
identification of microorganism was achieved at high-level accuracy:
over 92.8% for both Gram species and antibiotic-resistant species,
with 90.35% accuracy for the detection of mixed microorganism in infected
wound. This novel method provides a rapid and accurate method for
microbial classification, potentially aiding in the diagnosis and
treatment of infectious diseases.