An immimohistochemical study of regulatory peptides in lungs of amphibians

Cutz, Ernest; Goniakowska-Witalińska, Lucyna; Chan, Wing‐Lok

doi:10.1007/bf00218403

Cited by 37 publications

(25 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, they were also described less often in the respiratory epithelium near the ciliated epithelium. Studies indicate that 5-HT in PNECs may be involved in respiratory circulation by contracting the smooth muscles of blood vessels through a paracrine/endocrine pathway, and may also regulate mucus secretion and cilia movement in ciliated cells (Cutz et al, 1986;Beorlegui et al, 1994;Goniakowska-Witalinska, 1997). In addition, Watts et al (1994) suggested that 5-HT-induced contraction occurred in the smooth muscle cells of the trachea in guinea pigs.…”

Section: Discussionmentioning

confidence: 98%

“…A limited number of bombesin-IR single cells were found at the base of ciliated epithelium in the lungs of lower vertebrates, e.g., T. alpestris, H. nebulosus tokyoensis and R. temporaria (Cutz et al, 1986;Gomi et al, 1994;Bodegas et al, 1995). Cutz et al (1986) did not observe any bombesin immunoreactivity in the lung epithelium of B. bombina, B. variegata, R. lessonae, B. viridis and H. arborea, but did find numerous nerve fibres between the smooth muscles in the connective tissue.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The responses of pulmonary neuroendocrine cells in the lungs of Rana ridibunda to different experimental conditions

Öztay¹

2006

Animal Biol

View full text Add to dashboard Cite

The study investigated the localisation and immunohistochemistry of neuroendocrine cells (PNECs) and their behaviour in the lungs of Rana ridibunda under experimental conditions and compared with control lungs. Serotonin-and bombesin-immunoreactive (IR) PNECs were observed as solitary cells or clusters of cells at the base of ciliated epithelium on the dilated apical parts of primary septa where incoming air first arrives, and in the respiratory epithelium. Serotonin-IR PNECs were predominantly present in the ciliated epithelium while bombesin-IR PNECs were found in the ciliated and respiratory epithelium. Additionally, bombesin-IR nerve fibres were found in the epithelium and among smooth muscle cells in the connective tissue. In frogs kept in a dry aquarium, serotoninand bombesin-IR PNECs were characterised by various stages of secretion. Serotonin-IR PNECs released most of their secretory material, while bombesin-IR PNECs were stimulated for secretion. Moreover, bombesin secretions were raised. In conclusion, the cells were identified as members of the pulmonary epithelial endocrine system and can contribute to the pulmonary biology through a paracrine/endocrine pathway in the lungs of R. ridibunda.

show abstract

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

The responses of pulmonary neuroendocrine cells in the lungs of Rana ridibunda to different experimental conditions

Öztay¹

2006

Animal Biol

View full text Add to dashboard Cite

show abstract

“…Whereas it is absent from amphibian lungs (Cutz et al, 1986), immunostaining against calcitonin can be used as a reliable marker for NEB in Basiliscus uittatus, being even better than serotonin.…”

Section: The Cytochemical Staining Patternmentioning

confidence: 99%

“…Interestingly, the reduced staining intensities observed in some lower vertebrates for neuron specific enolase (NSE) in NEB but not in nerves (Cutz et al;, 1986;Scheuermann et al, 1989), also points to a reduced need for a protein being common for the neuroendocrine and the nervous system (Jackson et al, 1985). Therefore, the intriguing possibility arises that the variability for both PGP 9.5 and NSE, and maybe for argyrophilia, is parallel and thus might have the same origin.…”

Section: The Cytochemical Staining Patternmentioning

confidence: 99%

Neuroepithelial bodies in the lung of Basiliscus vittatus (Reptilia, Iguanidae)

Steen¹,

Lauweryns²

1994

Anat. Rec.

View full text Add to dashboard Cite

Beyond the confirmation that morphology, content of biologically active substances such as serotonin and calcitonin, and innervation are evolutionary well preserved features of NEB, the results reveal some intriguing features of B. vittatus NEB: strict separation of calcitonin and CGRP, reduced need for the de-ubiquitinating enzyme PGP 9.5, lack of luminal contact, and the basket-like innervation. The latter two properties possibly refer to a mechanoreceptor function of NEB in this species.

show abstract

“…They were identified by their content of 5-hydroxytryptamine (5-HT), which was demonstrated both microspectrofluorimetrically and immunocytochemically, as well as by the occurrence of neurosecretory granules Haller 1978, 1980;Wasano and Yamamoto 1978;Cutz et al 1986;Goniakowska-Witalińska 1986;Scheuermann et al 1989, Goniakowska-Witalińska and Cutz 1990, Bodegas et al 1993.…”

Section: Introductionmentioning

confidence: 99%

Neuroepithelial bodies in the lung of Melanophryniscus stelzneri stelzneri (Anura, Bufonidae)

Hermida

Farías²,

Fiorito

2003

Anatomy and Embryology

View full text Add to dashboard Cite

Electron microscopy of the lungs of Melanophryniscus stelzneri stelzneri (Anura) revealed the presence of a complex pattern of corpuscular cells (CCs). The respiratory surface over the septa presents small areas where the CCs are grouped forming neuroepithelial bodies (NEBs). These corpuscular structures can also be localized in the inner layer of the lung wall. Although in both cases NEBs protrude slightly into the airway lumen, they are separated from the airway lumen and the basal connective tissue by thin apical and basal cytoplasmic processes of neighbouring pneumocytes. Ultrastructurally, the CCs possess a large nucleus, clear cytoplasm and vesicles of variable morphology and size, containing an electron dense material surrounded by a lucent space in some cases. The size of these dense-core vesicles (DCVs) ranged from 40 to 100 nm. The NEBs are associated with afferent and efferent terminal nerves. These types of nerve endings are located between the CCs and in the basal part of the NEBs. The location of the NEBs in strategic positions on the septa and in the wall of the lung, the presence of the DCVs in the basolateral region of the CCs, the occurrence of synaptic contacts between nerve endings and the CCs and the occurrence of capillaries close to the NEBs, suggest a receptosecretory function for NEBs in the lung of M.s. stelzneri.

show abstract

An immimohistochemical study of regulatory peptides in lungs of amphibians

Cited by 37 publications

References 24 publications

The responses of pulmonary neuroendocrine cells in the lungs of Rana ridibunda to different experimental conditions

The responses of pulmonary neuroendocrine cells in the lungs of Rana ridibunda to different experimental conditions

Neuroepithelial bodies in the lung of Basiliscus vittatus (Reptilia, Iguanidae)

Neuroepithelial bodies in the lung of Melanophryniscus stelzneri stelzneri (Anura, Bufonidae)

Contact Info

Product

Resources

About