An Immune-Related Signature Predicted Survival in Patients With Kidney Papillary Cell Carcinoma

Shen, Junwen; Wang, Rongjiang; Chen, Yu; Fang, Zhihai; Tang, Jun; Yao, Jianxiang; Ling, Yuhang; Zhang, Lisha; Zhang, Xu

doi:10.3389/fonc.2021.670047

Cited by 5 publications

(5 citation statements)

References 27 publications

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“…Th1 cells mainly produce cytokines that defend against the tumor, such as TNF-a and IFN-γ, while Th2 cells promote tumor growth by releasing cytokines that inhibit the antitumor immune responses [28]. Though several studies have claimed BIRC5 was related to immune signature in several solid tumors, few of them researched the detailed mechanisms [23,[29][30][31]. In this study, we analyzed the data from the TCGA-LUAD cohort and found the high BIRC5 level was associated with chemokine activity, cytokine activity, and pathways in immune cells chemotaxis, which is deeply involved in TME of LUAD [32][33][34].…”

Section: Discussionmentioning

confidence: 99%

BIRC5 Modulates PD-L1 Expression and Immune Infiltration in Lung Adenocarcinoma

Ma¹,

Gu²,

Wen³

et al. 2022

J. Cancer

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Background: Lung adenocarcinoma (LUAD) is the most prevalent thoracic cancer with the highest incidence and mortality worldwide. Baculoviral IAP Repeat Containing 5 (BIRC5) is well studied in many malignancies, its prognosis value and correlation with the tumor microenvironment (TME) in LAUD remains largely elusive. Methods: The Wilcoxon signed-rank test and logistic regression were used to evaluate the relationship between clinical features and BIRC5 expression in LUAD. To assess the impact of BIRC5 on prognosis, the Kaplan-Meier plotter analysis and Cox regression were used, as well as a receiver operating characteristic (ROC) curve and nomogram. Gene set enrichment analysis (GSEA) and single-sample gene set enrichment analysis (ssGSEA) were recruited to predict the association between BIRC5 and immune cell infiltrations. Furthermore, qRT-PCR and western bolt were utilized to confirm gene expression on mRNA and protein levels. The proliferation of A549 and H1299 cells was evaluated using CCK8 and EdU assay. Cell mobility was tested by transwell assay and wound healing assay. Detection of PD-L1 and infiltrated CD8 T cells in xenograft tumors was done by flow cytometry. Results: BIRC5 expression was found to be substantially greater in LUAD patients. According to KM-plotter analysis, patients with high levels of BIRC5 had shorter survival rates. Multivariate Cox analysis revealed that elevated BIRC5 expression was an independent risk factor for OS and PFS in LUAD patients. High BIRC5 expression was predicted to be associated with chemokine activity and immune cell chemotaxis, whereas ssGSEA suggested that BIRC5 is highly associated with CD8 T cell infiltration and PD-L1 levels. In vitro experiments suggested overexpression of BIRC5 promoted the proliferation, mobility, and PD-L1 level of A549 cells, and vice versa in H1299 cells. Furthermore, in vivo study suggested elevated tumor weight and PD-L1 levels in xenograft tumors generated from LLC cells with overexpressed BIRC5. Conclusion: BIRC5 promotes lung adenocarcinoma progression by modulating PD-L1 expression and inducing tumor immune evasion.

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Section: Discussionmentioning

confidence: 99%

BIRC5 Modulates PD-L1 Expression and Immune Infiltration in Lung Adenocarcinoma

Ma¹,

Gu²,

Wen³

et al. 2022

J. Cancer

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“…In addition, a five-year time-dependent ROC analysis revealed a satisfied AUC value of 0.92. Because the metabolism-related signature had a high AUC value, the sensitivity and specificity of this signature were higher than those of signatures reported by a few previous studies in renal cancer patients [ 24 – 25 ].…”

Section: Discussionmentioning

confidence: 61%

Fatty acid metabolism-related signature predicts survival in patients with clear cell renal carcinoma

Wang

Shen

Chen

et al. 2022

Aging

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Objective: To explore fatty acid metabolism-related genes and signature, which could predict survival outcomes of clear cell renal carcinoma (ccRCC) patients. Materials and Methods: Transcriptional and survival data of fatty acid genes in ccRCC patients were retrieved from UCSC Xena and Geo DataSets. We first performed Lasso Cox regression analysis to identify survival-related genes. These genes were then used to construct metabolic-related gene signature and risk score. Enrichment analysis and immune component and chemotherapy response prediction were also performed. Results: In total, five survival-related genes were identified: AGR2, HAO2, IGF2BP1, MCCD1 and OLFM4 (p < 0.05). A series of survival value analyses revealed survival-related signature and risk score, including KM analysis (training set: p < 0.001; test set: p = 0.008). Four clinical indexes (T stage, N stage, M stage, and pathology) were positively correlated with risk score. Time-dependent ROC analysis yielded AUC value of 0.813. Immune landscape analysis revealed that risk score was strongly correlated with TAM score and cytotoxic score. Patients with high risk score and TAM score or cytotoxic score had the shortest survival time. Finally, inhibition of fatty acid metabolism in human ccRCC cell line produced corresponding changes in five genes, consistent with our preliminary results. Conclusion: We identified five survival-related genes (AGR2, HAO2, IGF2BP1, MCCD1 and OLFM4) in ccRCC patients. Our results also indicated that survival-related signature based on these genes is a potential robust prognostic biomarker for ccRCC in patients.

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“…The performance of the signature was compared with four other renal cancer signatures. Results showed that our signature had the highest AUC value based on the ROC and maximum area under the curve in DCA, suggesting that TAN phenotype has a more significant function in ccRcc patients compared with the immune phenotype [ 33 ], fatty acid metabolism [ 34 ], amino acid metabolism [ 35 ], and autophagy [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

Prognostic significance and mechanisms of CXCL genes in clear cell renal cell carcinoma

Shen¹,

Wang²,

Chen³

et al. 2023

Aging

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This study aimed to investigate the clinical significance, biological functions, and underlying mechanisms of CXCL genes in clear cell renal cell carcinoma (ccRcc) based on patient datasets and pan-cancer analysis. The interaction between CXCL genes in ccRcc and immune components, particularly in relation to neutrophil recruitment and polarization mechanisms, was also evaluated. Furthermore, a risk score was developed using a signature for neutrophil polarization. The role of CXCL2 was assessed through in vitro experiments. Results showed that five CXCL genes (CXCL 2, 5, 9, 10, and 11) were upregulated in renal cancer tissue, while seven genes (CXCL 1, 2, 3, 5, 8, 13, and 14) significantly impacted patient survival. Moreover, CXCL 1, 5, and 13 affected progression-free survival. Besides, differences in mRNA expression and immune components affected renal cancer outcomes. Furthermore, three pairs of CXCL gene-immune cell interactions (CXCL13-CD8+ T cells, CXCL9/10-M1 cells, CXCL1/2/3/8-neutrophils) were identified through single-cell and pan-cancer analysis. A TAN risk score with prognostic value for KIRC patients was constructed using 11 genes and a TAN signature. Neutrophil polarization significantly impacted survival. Notably, CXCL2 was involved in neutrophil recruitment and polarization, thus promoting ccRcc progression. In conclusion, seven prognostic CXCL genes (CXCL 1/2/3/5/8/13/14) for ccRcc patients and three pairs of CXCL gene-immune cell interactions were identified. Furthermore, results showed that CXCL 2 promotes ccRcc progression through neutrophil recruitment and polarization.

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An Immune-Related Signature Predicted Survival in Patients With Kidney Papillary Cell Carcinoma

Cited by 5 publications

References 27 publications

BIRC5 Modulates PD-L1 Expression and Immune Infiltration in Lung Adenocarcinoma

BIRC5 Modulates PD-L1 Expression and Immune Infiltration in Lung Adenocarcinoma

Fatty acid metabolism-related signature predicts survival in patients with clear cell renal carcinoma

Prognostic significance and mechanisms of CXCL genes in clear cell renal cell carcinoma

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