An Immuno- and Enzyme Cytochemical Study of the H+-K+ ATPase in Human Parietal Cells after Administration of Tetragastrin and Omeprazole.

Kobayashi, Akihiro; Araki, Keijiro; Ando, Tomoyuki; Ogata, Takuro

doi:10.1679/aohc.60.205

Cited by 3 publications

(2 citation statements)

References 22 publications

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“…This transitional substructure of the membranes is interpreted to be initial dilution of the apical plasma membrane by the massive recruitment of tubulovesicular membrane. (5) Immunocytochemical localization of H ϩ /K ϩ -ATPase (Hanzel et al, 1989;Kobayashi et al, 1997;Sachs, 1987;Smolka et al, 1983) indicated that this enzyme is located on both the cytoplasmic and plasma membranes. (Fig.…”

Section: Connection Between the Tubulocisternal Network And The Intramentioning

confidence: 99%

Morphological studies on the translocation of tubulovesicular system toward the intracellular canaliculus during stimulation of the gastric parietal cell

Ogata¹,

Yamasaki²

2000

Microsc. Res. Tech.

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The gastric parietal has two characteristic membrane systems. One is the intracellular canaliculus, which is specialized networks of enfolded luminal membrane channels lined with numerous microvilli. The other structures common to all parietal cells are the tubulovesicles or the tubulovesicular membranes, a system of tubules and vesicles. The tubulovesicular compartment is drastically depleted during maximal gastric acid secretion and this is coincident with an increase in the canalicular cell surface membrane. A plausible explanation for this redistribution is the fusion and transfer of tubulovesicular membranes to the plasma membrane. However, for many years there was no convincing evidence of connections between these two membrane systems. The mechanism of the transformation of tubulovesicular membrane into the plasma membrane without demonstrable connections has been an enigma to electron microscopists. Using a recently developed fixation technique for parietal cells [Sugai et al. (1995) Acta Anat Nippon 74:S101], we have investigated the organization of the cytoplasmic membrane systems in the rat resting and tetragastrin stimulated stomachs by ultra‐high‐resolution scanning electron microscopy (SEM). Gastric mucosae were microwave‐fixed in a cacodylate buffer, (334 milliosmoles/kgH2O (mOsm)), to which 1.0% glutaraldehyde and 0.5% formaldehyde were added. Specimens examined by TEM of thin sections revealed the cytoplasm packed with tubular membranes similar to images detected by rapid‐freeze/freeze‐substitution fixation. To render the cytoplasmic membranes visible by SEM, fixed mucosae were treated by the aldehyde‐osmium‐DMSO‐osmium maceration procedure. With much of the cell matrix and filaments removed, SEM revealed numerous 30–60‐nm tubules, which formed a meshwork with small cisternae. Vesicles or isolated tubules were not found in adequately macerated parietal cells. The cytoplasmic surface of the intracellular canaliculus was smooth except for round openings representing the bases of macerated microvilli. In favorable sites, connections of the tubular membranes to the canaliculi were clearly visible. Stereo pair views were particularly useful to demonstrate these continuities. Connections between these two membrane compartments suggest the probability of rapid membrane transposition. In this article, the form and distribution of membrane systems of parietal cells in the resting state and after tetragastrin stimulation will be presented and discussed. Special emphasis is made to demonstrate connections between the tubulovesicular system and the intracellular canaliculus. Microsc. Res. Tech. 48:282–292, 2000 © 2000 Wiley‐Liss, Inc.

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Section: Connection Between the Tubulocisternal Network And The Intramentioning

confidence: 99%

Morphological studies on the translocation of tubulovesicular system toward the intracellular canaliculus during stimulation of the gastric parietal cell

Ogata¹,

Yamasaki²

2000

Microsc. Res. Tech.

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“…There is further evidence that these two membrane compartments share similar properties. Immunocytochemical localization of H ϩ K ϩ ATPase (Smolka et al, 1983;Hanzel et al, 1989;Kobayashi et al, 1997) indicated that this enzyme is located on both the cytoplasmic and plasma membranes. Furthermore, freeze-fracture images of these two groups of membranes display a similar distribution of intramembrane particles (Forte et al, 1981).…”

Section: Discussionmentioning

confidence: 99%

Scanning EM of resting gastric parietal cells reveals a network of cytoplasmic tubules and cisternae connected to the intracellular canaliculus

Ogata¹,

Yamasaki²

2000

Anat. Rec.

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Using a recently developed fixation technique for parietal cells (Sugai et al., Acta Anat Nippon 1995:70:S79, 1999:74:S101), we have reinvestigated the organization of the cytoplasmic membrane system in the resting stomach by ultra-high-resolution scanning electron microscopy (SEM). Rat gastric mucosae were microwave-fixed in cacodylate buffer [334 milliosmoles/kg H(2)O (mOsm)], to which 1.0% glutaraldehyde and 0.5% formaldehyde were added. Specimens examined by transmission electron microscopy (TEM) of thin sections revealed cytoplasm packed with tubular membranes similar to images detected by rapid-freeze/freeze-substitution fixation which is generally considered to cause minimal structural alterations. To render the cytoplasmic membranes visible by SEM, fixed mucosae were frozen, fractured, and the exposed cytoplasm of parietal cells was macerated by the aldehyde-osmium-DMSO-osmium procedure. With much of the cell matrix and filaments removed, SEM revealed numerous 30-60 nm tubules which formed a meshwork and also small cisternae. The cytoplasmic surface of the tubules was smooth while some cisternal areas had attached polyribosomes. Vesicles or isolated tubules were not found in appropriately macerated parietal cells. The cytoplasmic surface of the intracellular canaliculus was smooth except for round openings representing the bases of macerated microvilli. In favorable sites connections of the tubular membranes to the canaliculi were clearly visible. Stereo pair views were particularly useful to demonstrate these continuities. Connections between these two membrane compartments suggest the probability of rapid membrane transposition.

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Scanning EM Studies of Gastric Oxyntic Cells with Special Reference to the Translocation of Tubulovesicular System towards the Intracellular Canaliculus

Ogata

2002

Mechanisms and Consequences of Proton Transport

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An Immuno- and Enzyme Cytochemical Study of the H+-K+ ATPase in Human Parietal Cells after Administration of Tetragastrin and Omeprazole.

Cited by 3 publications

References 22 publications

Morphological studies on the translocation of tubulovesicular system toward the intracellular canaliculus during stimulation of the gastric parietal cell

Morphological studies on the translocation of tubulovesicular system toward the intracellular canaliculus during stimulation of the gastric parietal cell

Scanning EM of resting gastric parietal cells reveals a network of cytoplasmic tubules and cisternae connected to the intracellular canaliculus

Scanning EM Studies of Gastric Oxyntic Cells with Special Reference to the Translocation of Tubulovesicular System towards the Intracellular Canaliculus

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