An immunoassay-based reverse-transcription loop-mediated isothermal amplification assay for the rapid detection of avian influenza H5N1 virus viremia

Tang, Yi; Yu, Xu; Chen, Hao; Diao, Youxiang

doi:10.1016/j.bios.2016.06.063

Cited by 27 publications

(14 citation statements)

References 36 publications

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“…positives are significantly reduced compared with existing methods. A further benefit of the real-time assay system is that it has the ability to be scaled up for high-throughput detection and to be used for accurate target quantification (Tang et al, 2016). The detection limit of the developed LAMP assay was compared with a conventional PCR assay system in the present study.…”

Section: Discussionmentioning

confidence: 99%

Development of a novel loop-mediated isothermal amplification assay for the detection of lipolytic Pseudomonas fluorescens in raw cow milk from north China

Liang

Zhang

et al. 2017

Journal of Dairy Science

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Lipases secreted by psychrotrophic bacteria are known to be heat resistant and can remain active even after the thermal processing of milk products. Such enzymes are able to destabilize the quality of milk products by causing a rancid flavor. Rapid detection of a small amount of heat-resistant lipase-producing psychrotrophic bacteria is crucial for reducing their adverse effects on milk quality. In this study, we established and optimized a novel loop-mediated isothermal amplification (LAMP) assay for the detection of Pseudomonas fluorescens in raw cow milk, as the most frequently reported heat-resistant lipase-producing bacterial species. Pseudomonas fluorescens-specific DNA primers for LAMP were designed based on the lipase gene sequence. Reaction conditions of the LAMP assay were tested and optimized. The detection limit of the optimized LAMP assay was found to be lower than that of a conventional PCR-based method. In pure culture, the detection limit of the LAMP assay was found to be 4.8 × 10 cfu/reaction of the template DNA, whereas the detection limit of the PCR method was 4.8 × 10 cfu/reaction. Evaluation of the performance of the method in P. fluorescens-contaminated pasteurized cow milk revealed a detection limit of 7.4 × 10 cfu/reaction, which was 10 lower than that of the PCR-based method. If further developed, the LAMP assay could offer a favorable on-farm alternative to existing technologies for the detection of psychotrophic bacterial contamination of milk, enabling improved quality control of milk and milk products.

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Section: Discussionmentioning

confidence: 99%

Development of a novel loop-mediated isothermal amplification assay for the detection of lipolytic Pseudomonas fluorescens in raw cow milk from north China

Liang

Zhang

et al. 2017

Journal of Dairy Science

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“…(3) For fish and other marine animals: koi herpes viruses [19,70,71], lymphocytis disease viruses for fish [54], infectious hematopoietic necrosis viruses (IHNV) in rain-bow trout [72], viral hemorrphagic septicemia viruses [73], infectious spleen and kidney necrosis viruses (ISKNV) in fishes [14,74], cyprinid herpesvirus 2 (CyHV-2) [75,76], cyprinid herpesvirus 3 [77,78], Edwardsiella tarda in fish [79], Francisella piscicida in Atlantic cod [80], iridovirus in fish [36,81,82], white spot syndrome virus in shrimp [44,[83][84][85], shrimp yellow head virus (YHV) [86,87], Taura syndrome virus (TSV) in shrimp [88], shrimp infectious myonecrosis virus (IMNV) [89,90], infectious hypodermal and hematopoietic necrosis virus (IHHNV) in shrimp [91,92], Penaeus monodon nucleopolyhedrovirus (PemoNPV) for shrimp [93], macrobrachium rosenbergii nodavirus (MrNV) and extra small virus (XSV) in prawn [94], acute viral necrobiotic virus in scallop [95], softshelled turtle iridovirus [96], abalone herpesviruses [39], mud crab dicistrovirus-1 [27], and ostreid herpesvirus 1 (OsHV-1) DNA [97]. (4) For birds and chicken: subgroup A and subgroup J of avian leukosis viruses [35,98], avian H5N1 viruses [99], infectious bursal disease viruses (IBDV) [15,…”

Section: Applications Of Lampmentioning

confidence: 99%

Colorimetric biosensors for point-of-care virus detections

Zhao

Wong

Zheng

et al. 2020

Materials Science for Energy Technologies

102

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a b s t r a c tColorimetric biosensors can be used to detect a particular analyte through color changes easily by naked eyes or simple portable optical detectors for quantitative measurement. Thus, it is highly attractive for point-of-care detections of harmful viruses to prevent potential pandemic outbreak, as antiviral medication must be administered in a timely fashion. This review paper summaries existing and emerging techniques that can be employed to detect viruses through colorimetric assay design with detailed discussion of their sensing principles, performances as well as pros and cons, with an aim to provide guideline on the selection of suitable colorimetric biosensors for detecting different species of viruses. Among the colorimetric methods for virus detections, loop-mediated isothermal amplification (LAMP) method is more favourable for its faster detection, high efficiency, cheaper cost, and more reliable with high reproducible assay results. Nanoparticle-based colorimetric biosensors, on the other hand, are most suitable to be fabricated into lateral flow or lab-on-a-chip devices, and can be coupled with LAMP or portable PCR systems for highly sensitive on-site detection of viruses, which is very critical for early diagnosis of virus infections and to prevent outbreak in a swift and controlled manner.

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“…Rapid influenza diagnostic tests (RIDTs) are widely used to identify IAVs in healthcare services because they are simple and swift [8][9][10] to 90%, the defect is an inconsistent sensitivity (10-80%) [8,9,11]. More recently, nucleic acid amplification tests (NAATs), such as reverse transcription polymerase chain reaction (RT-PCR) [12][13][14][15], real-time RT-PCR [16,17], and reverse transcription loop-mediated isothermal amplification (RT-LAMP) [18,19], have been used for rapid and sensitive diagnosis or subtyping of IAVs. Nevertheless, these methods require expensive equipment and/or skilled technicians, making them inappropriate for use in developing countries.…”

Section: Introductionmentioning

confidence: 99%

Reverse transcription recombinase polymerase amplification with lateral flow dipsticks for detection of influenza A virus and subtyping of H1 and H3

Sun

Wang

et al. 2018

Molecular and Cellular Probes

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Three reverse transcription recombinase polymerase amplification assays with lateral flow dipsticks (RT-RPA-LFD) were developed for identification of the matrix and hemagglutinin (HA) genes to detect influenza A virus and distinguish subtypes H1 and H3. Assessment of the assays' specificity showed that there was no crossreactivity with other targets. Their limits of detection were 123.6 copies per reaction for the matrix gene, 677.1 copies per reaction for the H1 HA gene, and 112.2 copies/reaction for the H3 HA gene. Of 111 samples tested by RT-RPA-LFD assays, 27 were positive for influenza A virus, 14 were positive for H1, and 10 were positive for H3. Compared to the results obtained from real-time RT-PCR assays, the sensitivity of RT-RPA-LFD assays was 75%, 93.33% and 71.43% for the matrix, H1, and H3, with 100% specificity. The sensitivity of RT-RPA-LFD assays is lower than that of real-time RT-PCR, comparable or better than that of conventional RT-PCR, and much better than that of RIDTs. In conclusion, these assays offer an efficient and reliable tool for identification and subtyping of influenza A virus (subtype H1 and H3) in the resource-limited setting.

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An immunoassay-based reverse-transcription loop-mediated isothermal amplification assay for the rapid detection of avian influenza H5N1 virus viremia

Cited by 27 publications

References 36 publications

Development of a novel loop-mediated isothermal amplification assay for the detection of lipolytic Pseudomonas fluorescens in raw cow milk from north China

Development of a novel loop-mediated isothermal amplification assay for the detection of lipolytic Pseudomonas fluorescens in raw cow milk from north China

Colorimetric biosensors for point-of-care virus detections

Reverse transcription recombinase polymerase amplification with lateral flow dipsticks for detection of influenza A virus and subtyping of H1 and H3

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