An Immunocytochemical Study of Interchromatin Granule Clusters in Early Mouse Embryos

Bogolyubova, I. O.; Bogolyubov, D. S.

doi:10.1155/2013/931564

Cited by 3 publications

(9 citation statements)

References 37 publications

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“…The data obtain by indirect immunofluorescent microscopy (Table) suggest that the molecular composition of the NPB surrounding heterochromatin including some chromatinassociated proteins, changes significantly during realization of the major events of embryonic genome activation as well as after artificial suppression of transcription. For example, some factors involved in mRNA metabolism including the spicing factor SC35 and basal transcription factor TFIID are revealed in the NPBassociated chromatin at transcriptionally inert stages (Bogolyubova, Bogolyubov, 2013) (Fig. 3).…”

Section: Molecular Composition Dynamics Of Npb-surrounding Heterochromentioning

confidence: 99%

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Peculiarities of the molecular composition of heterochromatin associated with pronucleoli in mouse embryos

2019

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The nucleus of pre-implantation mammalian embryos is characterized by peculiar structural organization. At the initial stages of cleavage, the nucleus of the embryo contains the so-called nucleolus precursor bodies (NPBs) or pronucleoli rather than functionally active nucleoli. The NPBs are fibrillar electron-dense structures inactive in RNA synthesis. The vast majority of NPBs are surrounded by a ring-shaped zone of transcriptionally inactive heterochromatin. Intriguingly, these zones contain not only tri-methylated histone Н3K9me3 as an epigenetic mark of repressed chromatin but also acetylated histone H4K5ac, a well-known marker of active chromatin. Immunocytochemical data suggest that the molecular composition of this ‘ring heterochromatin’ in mouse embryos changes during the realization of embryonic genome activation events, as well as during artificial suppression of transcription. In zygotes, some factors of mRNA biogenesis including splicing factor SC35 (SRSF2) and basal transcription factor TFIID are detectable in the ring chromatin. At later stages of development, other nuclear proteins such as Y14, a core component of the exon-exon junction complex (EJC), as well as the proteins involved in chromatin remodeling (ATRX, Daxx) are also detectable in this area. A typical component of the ‘ring heterochromatin’ is actin. Anti-actin immunocytochemical labeling is most expressed at the two-cell cleavage stage after activation of the embryonic genome. Indicatively, the molecular composition of the ‘ring heterochromatin’ associated with different NPBs may differ significantly even in the same nucleus. This seems to reflect the functional heterogeneity of morphologically similar NPBs according to their competence to the process of nucleologenesis. Here, we discuss briefly some peculiarities of the molecular composition and possible functions of the NPB-associated heterochromatin in mouse early embryos.

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Section: Molecular Composition Dynamics Of Npb-surrounding Heterochromentioning

confidence: 99%

“…Immunolocalization of the S/R-rich splicing factor SC35/SRSF2 in NPB-associated chromatin at an early stage (transcriptionally inert, 24 h post-hGG) of mouse development.Arrows indicate anti-SC35 labeling in the vicinity of NPBs. Original; inset is from(Bogolyubova, Bogolyubov, 2013), open access.…”

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confidence: 99%

Peculiarities of the molecular composition of heterochromatin associated with pronucleoli in mouse embryos

2019

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“…In our immunocytochemical experiments, we used an affinity purified polyclonal serum to reveal the hyperphosphorylated form of RNAP II in mouse embryos. This form of RNAP II was detected before the major ZGA phase, namely, in early 2-cell embryos [ 24 ]. While ZGA proceeds, RNAP II was found in association with perichromatin fibrils (PFs) [ 25 ], which are referred to as the ultrastructural “ in situ forms” of nascent pre-mRNA transcripts [ 55 ].…”

Section: Rna Polymerase II and Basal Transcription Factorsmentioning

confidence: 99%

“…Besides, the hyperphosphorylated RNAP II was detected in nuclear speckles identified by the presence of the SR protein SC35. The intensity of anti-RNAP II immunostaining in speckles was being increased towards the end of ZGA [ 24 ] ( Figure 1 ). If ZGA is delayed, for example, in embryos under the so-called “2-cell block in vitro ” (for details, see [ 56 , 57 ]), the hyperphosphorylated form of RNAP II begins to accumulate in enlarged nuclear speckles [ 26 ] ( Figure 2 ).…”

Section: Rna Polymerase II and Basal Transcription Factorsmentioning

confidence: 99%

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Nuclear Distribution of RNA Polymerase II and mRNA Processing Machinery in Early Mammalian Embryos

Bogolyubova

Bogolyubov

2014

BioMed Research International

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Spatial distribution of components of nuclear metabolism provides a significant impact on regulation of the processes of gene expression. While distribution of the key nuclear antigens and their association with the defined nuclear domains were thoroughly traced in mammalian somatic cells, similar data for the preimplantation embryos are scanty and fragmental. However, the period of cleavage is characterized by the most drastic and dynamic nuclear reorganizations accompanying zygotic gene activation. In this minireview, we try to summarize the results of studies concerning distribution of major factors involved in RNA polymerase II-dependent transcription, pre-mRNA splicing mRNA export that have been carried out on early embryos of mammals.

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Chromatin Configuration in Diplotene Mouse and Human Oocytes during the Period of Transcriptional Activity Extinction

Bogolyubova

Salimov²,

Bogolyubov

2023

IJMS

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In the oocyte nucleus, called the germinal vesicle (GV) at the prolonged diplotene stage of the meiotic prophase, chromatin undergoes a global rearrangement, which is often accompanied by the cessation of its transcriptional activity. In many mammals, including mice and humans, chromatin condenses around a special nuclear organelle called the atypical nucleolus or formerly nucleolus-like body. Chromatin configuration is an important indicator of the quality of GV oocytes and largely predicts their ability to resume meiosis and successful embryonic development. In mice, GV oocytes are traditionally divided into the NSN (non-surrounded nucleolus) and SN (surrounded nucleolus) based on the specific chromatin configuration. The NSN–SN transition is a key event in mouse oogenesis and the main prerequisite for the normal development of the embryo. As for humans, there is no single nomenclature for the chromatin configuration at the GV stage. This often leads to discrepancies and misunderstandings, the overcoming of which should expand the scope of the application of mouse oocytes as a model for developing new methods for assessing and improving the quality of human oocytes. As a first approximation and with a certain proviso, the mouse NSN/SN classification can be used for the primary characterization of human GV oocytes. The task of this review is to analyze and discuss the existing classifications of chromatin configuration in mouse and human GV oocytes with an emphasis on transcriptional activity extinction at the end of oocyte growth.

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An Immunocytochemical Study of Interchromatin Granule Clusters in Early Mouse Embryos

Cited by 3 publications

References 37 publications

Peculiarities of the molecular composition of heterochromatin associated with pronucleoli in mouse embryos

Peculiarities of the molecular composition of heterochromatin associated with pronucleoli in mouse embryos

Nuclear Distribution of RNA Polymerase II and mRNA Processing Machinery in Early Mammalian Embryos

Chromatin Configuration in Diplotene Mouse and Human Oocytes during the Period of Transcriptional Activity Extinction

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