An immunofluorescence study of the action of antibody in experimental intracerebral infection of mice with <i>Bordetella pertussis</i>

Iida, Tsuyoshi; Kusano, Nobuchika; Yamamoto, Akihiko; Konosu, Masafumi

doi:10.1002/path.1700920213

Cited by 6 publications

(7 citation statements)

References 12 publications

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“…In non-protected mice the bacteria continue to multiply until the viable count reaches 10' to I O~ organisms/brain, when the B. pertussis 'bactericidal antigen' 379 animal dies; in mice which have been protected by active immunization, however, the viable count starts to decline at about 4 days after challenge, and by 7 to 10 days after challenge the brain is sterile. At about the same time as the brain count in protected mice is beginning to decline the blood-brain barrier becomes permeable to bovine serum albumin (Berenbaum et al 1960), to the dye Pontamine Sky Blue, and to human serum (Holt, Spasojevic, Dolby & Standfast, 1961), and to horse gammaglobulin (Iida, Kusano, Yamamoto &Konosu, 1966). The blood-brain barrier is normally impermeable to all these substances and, at least in the case of Pontamine Sky Blue, becomes so again in surviving mice at about the same time as the brain becomes sterile.…”

Section: Discussionmentioning

confidence: 99%

The Antigen of Bordetella pertussis that Induces Bactericidal Antibody and its Relationship to Protection of Mice

Ackers

Dolby

1972

Journal of General Microbiology

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S U M M A R YThe ability to protect mice against intracerebral infection and to elicit, after one dose, complement-mediated bactericidal antibody capable of killing a mousevirulent phase I strain in vitro, was tested in seven cultures of Bordetellapertussis: three typical phase I strains; two strains grown in the presence of 0.5 mg nicotinic acid/ml ; one phase IV strain ; and the atypical strain 134. The typical strains showed both activities; the nicotinic acid-grown and phase IV strains elicited high antibody titres, but did not confer protection, whereas strain 134 protected, but did not elicit bactericidal antibody.Pyrogenic material extracted from dried organisms by hot phenol and water (lipopolysaccharide) was not antigenic in mice; however, with all strains, except 134, multiple doses of this material coupled to stromata or Escherichiu coli conjugated protein elicited bactericidal and precipitating antisera in mice, although the mice were not protected.Strain 134 contained approximately one quarter of the normal amount of endotoxin, as assayed in actinomycin D-sensitized mice, but was as effective as normal strains as an adjuvant for haemolysin production.

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Section: Discussionmentioning

confidence: 99%

The Antigen of Bordetella pertussis that Induces Bactericidal Antibody and its Relationship to Protection of Mice

Ackers

Dolby

1972

Journal of General Microbiology

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“…An intracranial challenge with Bordetella pertussis , administered into the ventricular cavity of experimental animals, results in the characteristic binding of this bacillus to the ependymal cilia ( Berenbaum et al . 1960 ;Iida et al . 1966 ;Hopewell et al .…”

Section: Introductionmentioning

confidence: 99%

Localization of a G protein G_i2 in the cilia of rat ependyma, oviduct and trachea

Shinohara

Asano

Kato

et al. 1998

Eur J of Neuroscience

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In previous studies, the localization of a pertussis toxin-sensitive G protein was demonstrated in ependymal cilia, but the identification of the subtype of G protein was inconsistent. To clarify this issue, we studied the localization of Goalpha, Gi1alpha, Gi3alpha and Gi2alpha in the ciliated ependymal cells and in the cilia of some other tissues of rats using specific antibodies. The cilia of the ependymal cells that line the ventricular cavity of the brain were intensely immunoreactive for Gi2alpha, but not for Goalpha, Gi1alpha or Gi3alpha. Immunoblot analysis demonstrated higher levels of Gi2alpha in the ependymal cilia-rich pellet than in the motor area of the parietal cortex. At the ultrastructural level, the immunoreactivity specific for Gi2alpha was found predominantly in the cilia, but rarely in the microvilli or the basal bodies of ependymal cells. In cross-sections, the immunoreactivity specific for Gi2alpha was observed only in cell membranes, in particular, in the inner electron-dense leaflet of the trilaminar structure. In addition to that in the ependymal cilia, such specific localization of Gi2alpha was observed in the motile cilia in other tissues, including the oviduct and trachea. By contrast, the stereocilia in the ductus deferens were not immunopositive for Gi2alpha. These findings suggest that Gi2 might play an important role in the signal transduction in ciliary membrane-associated function(s) of the ependymal cells, oviduct and trachea.

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“…However, i.c. challenge of mice with viable B. pertussis mirrors the events in human pertussis: first, the organisms do not cause a blood or purulent infection during infection but adhere to the cilia of the bronchi during disease and to the cilia of cerebral ventricles in the assay (both respiratory and CNS cilia have a common ectodermal origin) (20). Second, only pertussis toxin (PT) antibodies, whether actively induced or passively administered, conferred protection against lethal infection in mice including the Food and Drug Administration assay (21,22).…”

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confidence: 99%

Toward a new vaccine for pertussis

Robbins¹,

Schneerson²,

Kübler-Kiełb

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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To overcome the limitations of the current pertussis vaccines, those of limited duration of action and failure to induce direct killing of Bordetella pertussis , a synthetic scheme was devised for preparing a conjugate vaccine composed of the Bordetella bronchiseptica core oligosaccharide with one terminal trisaccharide to aminooxylated BSA via their terminal ketodeoxyoctanate residues. Conjugate-induced antibodies, by a fraction of an estimated human dose injected into young outbred mice as a saline solution, were bactericidal against B. pertussis , and their titers correlated with their ELISA values. The carrier protein is planned to be genetically altered pertussis toxoid. Such conjugates are easy to prepare, stable, and should add both to the level and duration of immunity induced by current vaccine-induced pertussis antibodies and reduce the circulation of B. pertussis .

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An immunofluorescence study of the action of antibody in experimental intracerebral infection of mice with Bordetella pertussis

Cited by 6 publications

References 12 publications

The Antigen of Bordetella pertussis that Induces Bactericidal Antibody and its Relationship to Protection of Mice

The Antigen of Bordetella pertussis that Induces Bactericidal Antibody and its Relationship to Protection of Mice

Localization of a G protein G_i2 in the cilia of rat ependyma, oviduct and trachea

Toward a new vaccine for pertussis

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An immunofluorescence study of the action of antibody in experimental intracerebral infection of mice with Bordetella pertussis

Cited by 6 publications

References 12 publications

The Antigen of Bordetella pertussis that Induces Bactericidal Antibody and its Relationship to Protection of Mice

The Antigen of Bordetella pertussis that Induces Bactericidal Antibody and its Relationship to Protection of Mice

Localization of a G protein Gi2 in the cilia of rat ependyma, oviduct and trachea

Toward a new vaccine for pertussis

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Localization of a G protein G_i2 in the cilia of rat ependyma, oviduct and trachea