An Immunohistochemical Study of Feline Myocardial Fibrosis

Aupperle, H.; Baldauf, K; März, I.

doi:10.1016/j.jcpa.2010.12.003

Cited by 11 publications

(17 citation statements)

References 46 publications

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“…IL-1, -2, -4, -6, -18, IFN-γ, TNF-α and TGF-β, but also ECM remodelling enzymes (MMP-2, -3, 13, TIMP-1, -2 and -3), with generally higher mRNA concentrations in atria than in ventricles. These results are in line not only with our previous findings in dogs (Fonfara et al, 2013a, b), where we could also confirm protein expression and identified the cardiomyocytes as the source of these markers (own observations), but also with a recent immunohistological study undertaken on cats (Aupperle et al, 2011). It suggests continuous remodelling of the feline myocardium at any age.…”

Section: Discussionsupporting

confidence: 93%

Age- and gender-dependent myocardial transcription patterns of cytokines and extracellular matrix remodelling enzymes in cats with non-cardiac diseases

Fonfara

Hetzel

Hahn

et al. 2015

Experimental Gerontology

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Background:Age, genderand systemic diseases all influence cardiac function and remodelling.In cats, age and genderassociated myocardial remodelling and the effect of systemic diseases on the myocardium have so far not been studied. The aim of the study was therefore to investigate whetherrelevant cytokines and extracellular matrix (ECM) remodellingenzymesare expressed in the myocardium of cats with non-cardiac diseasesand whether transcription levels are influenced by age and gender. Methods:The study was performed on myocardial samples from 26 cats aged between 2 and 19 years that had died with non-cardiac diseases. Seventeen cats were female (2 entire), nine were male (1 entire). Of these, nine cats were diagnosed with diseases unlikely to affect the myocardium (control cats). The remaining 17 cats suffered from diseases with likely systemic effects.All hearts were assessed for any pathological changes, and the myocardium was analysed for interleukin (IL)-1, -2, -4, -6, -18, tumour necrosis factor (TNF)-, interferon(IFN)-, transforming growth factor (TGF)-, matrix metalloproteinase (MMP)-2, -3, -13, tissue inhibitor of MMP (TIMP)-1, -2 and -3 transcription using quantitative RT-PCR assays. Results:Despite the absence of any histological evidence of myocardial damage,inflammation and fibrosis, the myocardium of all cats was found to constitutively transcribe cytokines and ECM remodelling enzymes, with generally higher mRNA concentrations in atria than in ventricles.Young and male cats exhibited higher transcription levels throughout the myocardium in comparison to older and female cats. Furthermore, age-associated transcription pattern differed between male and female cats. Conclusion:The constitutive transcription of ECM remodellingenzymes suggests continuous myocardial remodelling throughout the entire life of a cat. The myocardium of young and malecats appears to be in a pro-inflammatory state, whereas in older and female cats the myocardium exhibits a reduced inflammatory reaction to systemic disease.Age-associated cardiac remodelling seems to be influenced bynon-hormonal factors inmale and female cats. Abstract: Background: Age, gender and systemic diseases all influence cardiac function and remodelling. In cats, age and gender associated myocardial remodelling and the effect of systemic diseases on the myocardium have so far not been studied. The aim of the study was therefore to investigate whether relevant cytokines and extracellular matrix (ECM) remodelling enzymes are expressed in the myocardium of cats with non-cardiac diseases and whether transcription levels are influenced by age and gender. Methods: The study was performed on myocardial samples from 26 cats aged between 2 and 19 years that had died with non-cardiac diseases. Seventeen cats were female (2 entire), nine were male (1 entire). Of these, nine cats were diagnosed with diseases unlikely to affect the myocardium (control cats). The remaining 17 cats suffered from diseases with likely systemic effects. All hearts were assessed fo...

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Section: Discussionsupporting

confidence: 93%

Age- and gender-dependent myocardial transcription patterns of cytokines and extracellular matrix remodelling enzymes in cats with non-cardiac diseases

Fonfara

Hetzel

Hahn

et al. 2015

Experimental Gerontology

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“…Positive reactions were revealed with 3‐amino‐9‐ethilcarbazole (AEC+Substrate‐Chromogen; Dakocytomation). Samples of feline healthy myocardium were used as a positive control, as previously reported . Negative controls were prepared with sections of myocardium treated as previously described, omitting the primary antibody.…”

Section: Methodssupporting

confidence: 82%

“…Recently, the interest in MMPs has increased in veterinary medicine in particular, and several studies focusing on their role in tumour development have been reported . The role of these enzymes has also been investigated in inflammatory and degenerative processes . In the 51 specimens of FEDs tested, MMP‐2 was markedly overexpressed in the spindle cells identified as reactive fibroblasts.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, endothelial cells are thought to secrete proteases, such as MMP‐2 and MMP‐9, in response to angiogenic stimuli to degrade their basement membrane and therefore promote the formation of new blood vessels . Imbalances and alterations in the expression of these proteins have already been hypothesized as pathogenetic events in different diseases, in veterinary and human medicine …”

Section: Introductionmentioning

confidence: 99%

“…21 Imbalances and alterations in the expression of these proteins have already been hypothesized as pathogenetic events in different diseases, in veterinary and human medicine. [22][23][24][25][26][27][28][29][30] The aim of this study was to investigate the expression of MMP-2, MMP-9 and TIMP-2 in FEDs to gain better insight concerning their role in these lesions and to understand whether a different expression or an alteration in their balance could be related to different lesion morphology. Moreover, the correlation of FFs and NF with the site of the lesion and the age of the animal was investigated.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Feline eosinophilic dermatoses: a retrospective immunohistochemical and ultrastructural study of extracellular matrix remodelling

Porcellato

Giontella

Mechelli

et al. 2014

Veterinary Dermatology

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Human heart failure with preserved ejection versus feline cardiomyopathy: what can we learn from both veterinary and human medicine?

et al. 2017

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Cardiovascular affections are a growing health burden in human populations. Recent advances in cardiology have improved treatments and outcomes for myocardial infarction and arrhythmias, but other conditions still remain poorly understood. To date, the classical approach to study cardiovascular diseases involves rodent models, despite their strong differences with human cardiac physiology. In this context, this review will focus on the common traits between human and feline cardiac diseases, namely heart failure with preserved ejection fraction and feline cardiomyopathies, respectively. These two affections share similar pathological patterns and epidemiological characteristics. An improved knowledge would be of interest for both human and feline patients and could lead to the establishment of a more accurate treatment and therapeutic strategy for medical doctors and veterinary practitioners.

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An Immunohistochemical Study of Feline Myocardial Fibrosis

Cited by 11 publications

References 46 publications

Age- and gender-dependent myocardial transcription patterns of cytokines and extracellular matrix remodelling enzymes in cats with non-cardiac diseases

Age- and gender-dependent myocardial transcription patterns of cytokines and extracellular matrix remodelling enzymes in cats with non-cardiac diseases

Feline eosinophilic dermatoses: a retrospective immunohistochemical and ultrastructural study of extracellular matrix remodelling

Human heart failure with preserved ejection versus feline cardiomyopathy: what can we learn from both veterinary and human medicine?

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