An immunohistochemical study of laminin in basal cell carcinoma

Mostafa, Wedad Z.; Mahfouz, Soheir; Bosseila, Manal; Sobhi, Rehab Mohamed; El‐Nabarawy, Eman

doi:10.1111/j.1600-0560.2009.01310.x

Cited by 9 publications

(11 citation statements)

References 19 publications

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“…These results are in accordance with previous studies by Tosios et al .,[ 8 ] Souza et al .,[ 7 ] and Mostafa et al .,[ 9 ] where positive continuous staining of laminin was observed around the basement membrane. This indicates that the cells adjacent to basement membrane and the tumor cells secrete laminin.…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical expression of basement membrane laminin in histological grades of oral squamous cell carcinoma: A semiquantitative analysis

R¹,

Sharada

Swaminathan

et al. 2013

J Oral Maxillofac Pathol

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Background and Objectives:To determine the immunohistochemical (IHC) localization of basement membrane component laminin in histological grades of oral squamous cell carcinoma (OSCC). The purpose of this study was to demonstrate the loss of continuity of the basement membrane in OSCC using an antibody directed against laminin using advanced polymer staining system.Materials and Methods:A total of 30 cases of OSCC: 10 cases of well differentiated squamous cell carcinom (WDSCC), 10 cases of moderately differentiated squamous cell carcinoma (MDSCC), and 10 cases of poorly differentiated squamous cell carcinoma (PDSCC) were subjected to heat-induced antigen retrieval method using ethylene-di-amine-tetraacetic acid buffer in a microwave oven. Then the sections were stained with anti-laminin polyclonal antibody and visualized using super sensitive polymer horseradish peroxidase detection system. In each case, the integrity of the basement membrane laminin was assessed by using statistical analysis.Results:Statistical analysis showed a decreased distribution of laminin from WDSCC to MDSCC to PDSCC (P value 0.0573). The intracytoplasmic staining of laminin gradually increased from WDSCC to MDSCC to PDSCC (P value 0.0198).Interpretation and Conclusion:WDSCC cases showed more laminin expression in basement membrane around the tumor islands and less loss of continuity compared to MDSCC and PDSCC cases suggesting a greater enzymatic degradation of basement membrane components in MDSCC and PDSCC than WDSCC. The loss of structural basement membrane laminin and the presence of laminin in the tumor cells of PDSCC cases suggest that laminin helps in tumor invasion. The expression of laminin in the basement membrane may be a useful parameter to evaluate tumor histologic differentiation and aggressiveness.

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Section: Discussionmentioning

confidence: 99%

Immunohistochemical expression of basement membrane laminin in histological grades of oral squamous cell carcinoma: A semiquantitative analysis

R¹,

Sharada

Swaminathan

et al. 2013

J Oral Maxillofac Pathol

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“…12 In addition, alterations in BM proteins expression contribute to development and progression of tumors. 13,14 Expression of cytoplasmic and BM laminin was increased with the degree of tumor cell differentiation in basal cell carcinoma. 14 Loss of collagen type IV has been observed in invasive prostate cancer.…”

Section: Introductionmentioning

confidence: 98%

“…13,14 Expression of cytoplasmic and BM laminin was increased with the degree of tumor cell differentiation in basal cell carcinoma. 14 Loss of collagen type IV has been observed in invasive prostate cancer. 13 BM proteins appear to play an important role in odontogenic tumors since many BM proteins are expressed in several types of odontogenic tumors.…”

Section: Introductionmentioning

confidence: 98%

Investigation of basement membrane proteins in a case of granular cell ameloblastoma

2012

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Granular cell ameloblastoma is a rare, benign neoplasm of the odontogenic epithelium. A case of massive granular cell ameloblastoma in a 44-year-old Thai female is reported. Histopathological features displayed a follicular type of ameloblastoma with an accumulation of granular cells residing within the tumor follicles. After treatment by partial mandibulectomy, the patient showed a good prognosis without recurrence in a 2-year follow-up. To characterize the granular cells in ameloblastoma, we examined the expression of basement membrane (BM) proteins, including collagen type IV, laminins 1 and 5 and fibronectin using immunohistochemistry. Except for the granular cells, the tumor cells demonstrated a similar expression of BM proteins compared to follicular and plexiform ameloblastomas in our previous study, whereas the granular cells showed strong positivity to laminins 1 and 5 and fibronectin. The increased fibronectin expression in granular cells suggests a possibility of age-related transformation of granular cells in ameloblastoma.

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“…The authors of the previous study also showed that patients with diffuse expression had a poorer prognosis than patients with the peripheral expression. Similar to SCC, the majority of cases of basal cell carcinoma also showed cytoplasmic expression of laminin‐5 in a recent study 21 …”

Section: Discussionmentioning

confidence: 59%

Expression of laminin-5 γ2 chain in cutaneous pseudocarcinomatous hyperplasia

Santos

Carneiro

Queiroz

et al. 2011

Journal of Cutaneous Pathology

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An immunohistochemical study of laminin in basal cell carcinoma

Abstract: Cytoplasmic and basement membrane laminin is important in the pathogenesis and invasion of BCC. Most laminin was in basement membrane zone (BMZ), and the more differentiated the tumor, the more cytoplasmic and BM staining it expressed.

Cited by 9 publications

References 19 publications

Immunohistochemical expression of basement membrane laminin in histological grades of oral squamous cell carcinoma: A semiquantitative analysis

Immunohistochemical expression of basement membrane laminin in histological grades of oral squamous cell carcinoma: A semiquantitative analysis

Investigation of basement membrane proteins in a case of granular cell ameloblastoma

Expression of laminin-5 γ2 chain in cutaneous pseudocarcinomatous hyperplasia

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