An immunohistochemical study of the pancreatic endocrine cells of the ddN mouse.

Lee, Hyeung Sik; Chang, Junghwan; Ku, Sae Kwang

doi:10.2478/v10042-010-0026-y

Cited by 13 publications

(15 citation statements)

References 21 publications

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“…All sections were rinsed 3 times in 0.01 M phosphate-buffered saline (PBS) between steps. Immunoreactive cell densities over 20% as compared with other naïve cells were regarded as positive, and the positive cells were assessed based on mean areas of pancreatic islets (mm 2 ) using the automated image analysis, as previously described (41,40). The ratios of insulin-positive/glucagon-positive cells were calculated as shown below in equation [2].…”

Section: Methodsmentioning

confidence: 99%

Blood glycemia-modulating effects of melanian snail protein hydrolysates in mice with type II diabetes

Choi¹,

Kim²,

Park³

et al. 2017

International Journal of Molecular Medicine

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Abstract. Freshwater animal proteins have long been used as nutrient supplements. In this study, melanian snail (Semisulcospira libertina) protein hydrolysates (MPh) were found to exert anti-diabetic and protective effects against liver and kidney damage in mice with type II diabetes adapted to a 45% kcal high-fat diet (HFD). The hypoglycemic, hepatoprotective and nephroprotective effects of MPh were analyzed after 12 weeks of the continuous oral administration of MPh at 125, 250 and 500 mg/kg. Diabetic control mice exhibited an increase in body weight, and blood glucose and insulin levels, with a decrease in serum high-density lipoprotein (HDL) levels. In addition, an increase in the regions of steatohepatitis, hepatocyte hypertrophy, and lipid droplet deposit-related renal tubular vacuolation degenerative lesions were detected, with noticeable expansion and hyperplasia of the pancreatic islets, and an increase in glucagon-and insulin-producing cells, insulin/glucagon cell ratios in the endocrine pancreas and hepatic lipid peroxidation, as well as decreased zymogen contents. Furthermore, a deterioration of the endogenous antioxidant defense system was observed, with reduced glucose utilization related hepatic glucokinase (GK) activity and an increase in hepatic gluconeogenesis-related phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase (G6pase) activity. However, all of these diabetic complications were significantly inhibited by oral treatment with MPh in a dose-dependent manner. In addition, the marked dose-dependent inhibition of hepatic lipid peroxidation, the depletion of the liver endogenous antioxidant defense system, and changes in hepatic glucose-regulating enzyme activities were also observed. The results of this study suggest that MPh exerts potent anti-diabetic effects, along with the amelioration of related complications in mice with type II diabetes. The overall effects of MPh at a dose of 125 mg/kg on HFD-induced diabetes and related complications were similar or more potent than those of metformin (250 mg/kg).

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Section: Methodsmentioning

confidence: 99%

Blood glycemia-modulating effects of melanian snail protein hydrolysates in mice with type II diabetes

Choi¹,

Kim²,

Park³

et al. 2017

International Journal of Molecular Medicine

Self Cite

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show abstract

“…All sections were rinsed 3 times between steps in 0.01 M phosphate-buffered saline (PBS). Cells with >20% immunoreactivity were regarded as positive compared with other naïve cells, and the mean numbers of insulin- and glucagon- immunoreactive cells dispersed in the pancreatic parenchyma were counted using an automated image analysis process, as previously described (39,47) and the ratio was calculated using equation [4] as follows:

\frac{Insulin}{Glucagon} cells (ratio) = \frac{Mean number of insulin - immunoreactive cells}{Mean number of glucagon immunoreactive cells}

…”

Section: Methodsmentioning

confidence: 99%

Anti-obesity effects of yellow catfish protein hydrolysate on mice fed a 45% kcal high-fat diet

Kim

Kim²,

Park

et al. 2017

International Journal of Molecular Medicine

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Obesity contributes to the etiologies of a variety of comorbid conditions, such as type 2 diabetes, hypertension and cardiovascular disease. In the present study, the anti-obesity effects of yellow catfish protein hydrolysate (YPh) were observed in mice fed a 45% kcal high-fat diet (HFD) compared with those of mice treated with simvastatin. The HFD-fed control mice exhibited noticeable increase in body weight, and whole-body and abdominal fat densities, periovarian and abdominal wall-deposited fat pad weight, as well as in the levels of triglycerides (TG), blood total cholesterol (TC), low-density lipoprotein, alanine aminotransferase, aspartate aminotransferase, creatinine, blood urea nitrogen, and in the fecal TG and TC contents. However, they exhibited a decrease in serum high-density lipoprotein levels. In addition, an increase was detected in periovarian and dorsal abdominally deposited fat pad thickness, adipocyte hypertrophy, the number of steatohepatitis regions, hepatocyte hypertrophy and lipid droplet deposition-related renal tubular vacuolation degenerative lesions, along with increased hepatic lipid peroxidation and a deteriorated endogenous antioxidant defense system (glutathione, catalase and superoxide dismutase). However, all the above-mentioned obesity-related complications were dose-dependently and significantly inhibited after 84 days of thye consecutive oral administration of 125, 250 and 500 mg/kg YPh. In addition, YPh dose-dependently depleted the liver endogenous antioxidant defense system and inhibited hepatic lipid peroxidation. Overall, the effects of 250 mg/kg YPh on HFD-induced obesity and related complications were similar or more potent than those of 10 mg/kg simvastatin. These results indicate that YPh is a promising new potent medicinal ingredient for possible use in the treatment of obesity and related complications.

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“…All sections were rinsed with 0.01 M PBS 3 times between each step. The cells with density over 20% of the immunoactivities as compared with other naïve cells, were regarded as positive, and the positive cells were assessed as mean numbers in the area of pancreatic islets (mm 2 ) using the automated image analysis process [37]. In addition, the ratio of insulin-positive to glucagon-positive cells was calculated.…”

Section: Methodsmentioning

confidence: 99%

Fermentation with Aquilariae Lignum Enhances the Anti-Diabetic Activity of Green Tea in Type II Diabetic db/db Mouse

et al. 2014

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The major components of tea may be significantly influenced according to the type of fermentation, and consequently the effects of different teas will differ. We examined whether green tea fermented with Aquilariae Lignum (fGT) shows a stronger anti-diabetic effect than unfermented green tea (GT) on mice with type 2 diabetes. To evaluate the anti-obesity effect of fGT, we assessed body weight, fecal excretion, serum leptin levels, exocrine pancreatic zymogen granule contents, and periovarian fat weight and adiponectin contents. Blood glucose levels, pancreatic weight, and numbers of pancreatic islet insulin- and glucagon-producing cells were determined to evaluate anti-hypoglycemic effects, while total cholesterol, triglyceride, and low- and high-density lipoprotein levels were determined to evaluate anti-hyperlipidemic effects. The antioxidant effect of fGT was detected by measuring malondialdehyde and glutathione contents and the activities of catalase and superoxide dismutase. fGT showed anti-obesity, anti-hypoglycemic, anti-hyperlipidemia, and antioxidant effects. Additionally, fGT exerted stronger anti-diabetic effects compared with GT. Collectively, these results suggested that fGT fermented with the appropriate amounts of Aquilariae Lignum (49:1) has a stronger effect compared with GT. Thus, fGT is a promising and potent new therapeutic agent for type 2 diabetes.

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An immunohistochemical study of the pancreatic endocrine cells of the ddN mouse.

Cited by 13 publications

References 21 publications

Blood glycemia-modulating effects of melanian snail protein hydrolysates in mice with type II diabetes

Blood glycemia-modulating effects of melanian snail protein hydrolysates in mice with type II diabetes

Anti-obesity effects of yellow catfish protein hydrolysate on mice fed a 45% kcal high-fat diet

Fermentation with Aquilariae Lignum Enhances the Anti-Diabetic Activity of Green Tea in Type II Diabetic db/db Mouse

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