An Immunohistochemical Study of the Localization of Biglycan, Decorin and Large Chondroitin-sulphate Proteoglycan in Pleomorphic Adenoma.

Abiko, Yoshihiro; Nishimura, Michiko; Mitamura, Jiro; Rahemtulla, Firoz; Scott, Paul; Dodd, Carole M.; Mizoguchi, Itaru; Kaku, Tohru

doi:10.1267/ahc.32.459

Cited by 3 publications

(1 citation statement)

References 22 publications

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“…Furthermore, it was found strong immunoreactivity in the epithelium of versican in the peripheral region of salivary pleomorphic adenoma [ 49 ]. High versican immunoexpression was observed in all kinds of odontogenic myxoma [ 50 ].…”

Section: Discussionmentioning

confidence: 99%

Gene Expression and Immunochemistry Analysis of ADAMTS-1 and Versican in Ameloblastoma

Neto

Fuzii

Silva

et al. 2022

International Journal of Dentistry

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Background. Ameloblastoma is a benign but locally invasive odontogenic epithelial tumor, associated with a high recurrence rate after treatment. The action of enzymes of the metalloproteinase family is important to the degraded extracellular matrix, contributing to invasion. Thus, this study aimed to investigate the gene and protein expression of ADAMTS-1 and versican in ameloblastoma. Materials and Methods. Twenty cases of ameloblastoma (n = 20) and ten dental follicles (DF) (n = 10) were used as a source for immunochemistry and quantitative RT-PCR for determining the protein and mRNA expressions of the concerned genes, respectively. Moreover, western blot and indirect immunofluorescence analysis were performed in AME cells. Results. ADAMTS-1 and versican were overexpressed in DF than ameloblastoma by RT-PCR. However, in the immunolocalization analysis, ADAMTS-1 was expressed in ameloblastoma more than in DF and versican immunostaining obtained a similar pattern between ameloblastoma and DF. Indirect immunofluorescence detected the ADAMTS-1 and versican expression in cell lines derived from ameloblastoma. Western blot from cell lysate and conditioned medium detected ADAMTS-1 bands representing full-length and different processed forms. Monensin treatment confined ADAMTS-1 in the cell cytoplasm. Versican fragments also were detected in different compartments, intracellular and conditioned medium, allowing the versican process by ADAMTS-1. Conclusion. This study showed a distinct expression of ADAMTS-1 and versican in ameloblastoma and DF, with ADAMTS-1 protein higher expression observed in ameloblastoma and possibly cleaved versican. These findings suggested that ADAMTS-1 may participate in tumor invasion, especially for the degradation of substrates (versican) in the ECM.

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Section: Discussionmentioning

confidence: 99%

Gene Expression and Immunochemistry Analysis of ADAMTS-1 and Versican in Ameloblastoma

Neto

Fuzii

Silva

et al. 2022

International Journal of Dentistry

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Immunohistochemical localization of large chondroitin sulfate proteoglycan in odontogenic tumor

Ito

Abiko²,

Tanaka

et al. 2002

Medical Electron Microscopy

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The present study investigated the localization of versican in odontogenic tumors by immunohistochemistry, using paraffin-embedded sections obtained from 27 patients with odontogenic tumors (17 ameloblastomas, 1 adenomatoid odontogenic tumor, 4 odontogenic keratocysts, 1 calcifying odontogenic cyst, 2 ameloblastic fibromas, and 2 malignant ameloblastomas). Deparaffinized sections were immersed in a buffered 1 : 1000 solution of an antibody, 5D5 (raised against a large chondroitin sulfate proteoglycan from bovine sclera), which mainly recognizes versican. All samples showed a positive reaction for versican in connective tissues, whereas positive staining of epithelial nests was observed in only some samples. The positive staining in epithelial nests was in areas showing stellate reticulum-like, cuboidal, columnar cells at the periphery, and tear-drop structures. These results indicated that versican might be involved in, at least in part, the morphogenesis of neoplastic epithelium and mesenchymal tissues in odontogenic tumors.

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Pleomorphic adenoma and carcinoma ex‐pleomorphic adenoma tumorigenesis: A proteomic analysis

Zanella,

Costa,

Schuch

et al. 2024

Oral Diseases

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ObjectivesTo conduct a comprehensive proteomic analysis of normal salivary gland tissue, pleomorphic adenoma (PA), and carcinoma ex‐pleomorphic adenoma (CXPA), and validate the proteomic findings using immunohistochemistry.MethodsSix normal salivary gland tissues, seven PA and seven CXPA samples underwent laser microdissection followed by liquid chromatography coupled to mass spectrometry. Protein identification and quantification were performed using MaxQuant software. Statistical analysis and functional enrichment were conducted using the Perseus platform and STRING tool, respectively. Immunohistochemistry was used for validation.ResultsComparative proteomic analysis revealed 2680 proteins across the three tissue types, with 799 significantly altered between groups. Translocation protein SEC63 homolog, Annexin A6 and Biglycan were up‐regulated in CXPA compared to PA. Decorin was markedly up‐regulated in both PA and CXPA compared to normal salivary gland (log2 fold changes of 7.58 and 7.38, respectively). Validation confirmed elevated levels of Biglycan and Decorin in the extracellular matrix of CXPA compared to PA.ConclusionsProteomic analysis identified differential protein expression patterns associated with malignant transformation of PA into CXPA. Findings indicate a crucial role for extracellular matrix proteins, specifically Biglycan and Decorin, in the tumorigenic progression of PA and CXPA.

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An Immunohistochemical Study of the Localization of Biglycan, Decorin and Large Chondroitin-sulphate Proteoglycan in Pleomorphic Adenoma.

Cited by 3 publications

References 22 publications

Gene Expression and Immunochemistry Analysis of ADAMTS-1 and Versican in Ameloblastoma

Gene Expression and Immunochemistry Analysis of ADAMTS-1 and Versican in Ameloblastoma

Immunohistochemical localization of large chondroitin sulfate proteoglycan in odontogenic tumor

Pleomorphic adenoma and carcinoma ex‐pleomorphic adenoma tumorigenesis: A proteomic analysis

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