An Immunologic Approach to Cell Cycle Analysis of the Stem Cell

Monette, Francis C.; Eichacker, Peter Q.; Byrt, William; Garver, Robert I.; Gilio, Michael J.; Demello, John B.

doi:10.1007/978-1-4612-9895-3_2

Cited by 3 publications

(3 citation statements)

References 22 publications

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“…Antigens such as Thy 1 (6) are known to be expressed in brain as well as on lymphohemopoietic cells, and many antibrain antisera crossreact with hemopoietic cells (7)(8)(9). Furthermore, antigenic crossreactivities have been reported between brain tissue and a pluripotential hemopoietic stem cell that give rise to hemopoietic colony formation in the spleen (CFU-s) (7).…”

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Pluripotential hemopoietic stem cells in adult mouse brain.

Bartlett¹

1982

Proc. Natl. Acad. Sci. U.S.A.

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Single cell suspensions ofadult mouse brain were shown to contain large numbers of pluripotential hemopoietic stem cells as detected by the ability to form hemopoietic colonies in the spleens of irradiated hosts. These colony forming unit, spleen (CFU-s) cells derived from brain gave rise to colonies identical in morphology and histology to those ofbone marrow-derived CFU-s. The average number of CFU-s obtained per 105 dissociated adult brain cells was 14, whereas other adult tissues such as lung, kidney, liver, heart, and thymus contained insignificant CFU-s levels when tested. As the level of CFU-s in adult blood is <1 per 106 nucleated cells, blood contamination does not contribute to the high levels found in adult brain. Individual spleen colonies isolated from irradiated CBA (H-2k) recipients injected with (BALB/c x CBA)F1 (H-2d x H-2k) brain cells were shown by immunofluorescence to contain cells bearing surface H-2d molecules, thus indicating that the colonies arose from the brain cell inoculum and were not endogenously derived. The surface phenotype of brainand bone marrow-derived CFU-s was found to differ in that brain CFU-s could be inhibited by prior incubation with a monoclonal antibrain antibody B2A2, whereas bone marrow CFU-s were not. Further differences were found between brain and bone marrow CFU-s in the congenitally anemic Wf/lW mice. These mice were shown to have very few CFU-s in the adult bone marrow, whereas the brain contained normal adult levels. The large number of hemopoietic stem cells in the brain may indicate an essential requirement for the continual generation of cells such as microglia or phagocytic cells, without the disruption of the blood-brain barrier.

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confidence: 99%

Pluripotential hemopoietic stem cells in adult mouse brain.

Bartlett¹

1982

Proc. Natl. Acad. Sci. U.S.A.

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“…This mixture was incubated for 30 min at 25 "C and then for 45 min at 4°C. The addition of complement was not required for the inhibitory effect [7]. The cells were then washed twice with Hanks' balanced salt solution.…”

Section: Stem Cell Assaysmentioning

confidence: 99%

Expression of cell surface determinant(s) on murine myeloma stem cells and hematopoietic stem cells

1984

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Murine B cell lymphomas and myelomas were examined for the expression of a determinant previously found exclusively on normal pluripotent stem cells colony-forming unit-spleen (CFU-s). This determinant(s), which is defined by a rabbit antimouse brain antiserum (R alpha MB), is present on the tumor stem cell population of some but not all B cell neoplasms examined. The determinant is not detected on tumor cells of the macrophage or T cell lineage. Absorption of the activity in R alpha MB with myeloma cells, concomitantly removed reactivity with the normal stem cell, CFU-s, and the myeloma stem cell, plasmacytoma CFU-s. Sorting analysis further showed that the antigen was diminished within a positive tumor population as cells acquired the capacity to secrete immunoglobulin. These studies suggest that this normal stem cell-associated antigen may also be an early differentiation antigen for the B cell lineage, and is expressed on some stem cells of B cell tumors.

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“…3 10.83 f 1.01 ( 6) 9.80 f 1.60( 5) Exp. 4 12.36 f 0.89 (11) 12.00 f 0.85( 9) Exp. 5 14.50 f 1.79 (12) 13.45 f 1.63 (11) % Loss due to HU 2.33 5.88 9.51 2.91 7.24 ( ) = number of spleens counted The suicide rate of CFU-s as a whole rose to 22.33% to 35.89% (p < 0.05), and that of the RAMBS-resistant CFU-s subpopulation was elevated to 40.08% to 60.06% (p < 0.01).…”

Section: Tables I and I1mentioning

confidence: 99%

Comparison of the effects of 4‐methylhistamine on the cell‐cycle response between CFU‐s and its subpopulation

Tang

1987

The International Journal of Cell Cloning

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When bone marrow cells were treated with an H2-receptor agonist (4-methylhistamine 10(-8) M) before treatment with hydroxyurea (10(-3) M), the suicide rate of spleen colony-forming units (CFU-s) as a whole rose significantly. The rabbit antimouse brain serum (RAMBS)-resistant CFU-s subpopulation was also significantly elevated. Prior treatment of bone marrow cells with cimetidine would prevent this effect of 4-methylhistamine (4-MH). These findings not only confirm that 4-MH can trigger mouse bone marrow CFU-s to enter a DNA synthetic phase of the cell cycle, but also suggest that the RAMBS-resistant CFU-s subpopulation is more sensitive to the effect of 4-MH. Furthermore, this suggests that concentrations of the histamine receptor change during the developmental process of CFU-s.

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An Immunologic Approach to Cell Cycle Analysis of the Stem Cell

Cited by 3 publications

References 22 publications

Pluripotential hemopoietic stem cells in adult mouse brain.

Pluripotential hemopoietic stem cells in adult mouse brain.

Expression of cell surface determinant(s) on murine myeloma stem cells and hematopoietic stem cells

Comparison of the effects of 4‐methylhistamine on the cell‐cycle response between CFU‐s and its subpopulation

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