An immunonanogold resonance scattering spectral probe for rapid assay of human chorionic gonadotrophin

Jiang, Zhiliang; Zou, Mingjing; Liang, Aihui

doi:10.1016/j.cca.2007.08.017

Cited by 29 publications

(22 citation statements)

References 25 publications

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“…Our group has accomplished a lot of work in this field. On the basis of the reaction of immunonanogold probe and antigen, which produced large immunocomplex, leading to the change of RS signal, the immunonanogold resonance scattering (INGRS) spectral methods for the detection of IgG [10], human chorionic gonadotrophin (hCG) [11], complement 3 [12], fibrinogen [13], APOA1 and APOB [14], prealbumin [15], Malb [16], IgA [17], etc., have been established successfully (Table 1). Compared to nanogold, nanosilver is of lower cost and easier to obtain.…”

Section: Nanogold and Nanosilver Particlesmentioning

confidence: 99%

Nanocatalytic resonance scattering spectral analysis

Jiang

Wen

et al. 2010

Sci. China Chem.

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The resonance scattering spectral technique has been established using the synchronous scanning technique on spectrofluorometry. Because of its advantages of simplicity, rapidity and sensitivity, it has been widely applied to analyses of proteins, nucleic acids and inorganic ions. This paper summarizes the application of immunonanogold and aptamer modified nanogold (AptAu) catalytic resonance scattering spectral technique in combination with the work of our group, citing 53 references.antibody, aptamer, nanocatalysis, resonance scattering spectral assay

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Section: Nanogold and Nanosilver Particlesmentioning

confidence: 99%

Nanocatalytic resonance scattering spectral analysis

Jiang

Wen

et al. 2010

Sci. China Chem.

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“…HCG secreted by trophoblast cells of placenta and trophoblast cells in gestational trophoblastic diseases play an important role in early diagnosis of all pregnancy tests and trophoblastic diseases, such as choriocarcinoma, ectopic pregnancy and hydatidiform moles [3]. As a result the early quantitative detection of HCG in female patients can predicte the pregnancy and trophoblastic diseases allowing timely medical intervention.…”

Section: Introductionmentioning

confidence: 99%

An Amperometric Immunosensor Based on Layer‐by‐Layer Assembly of L‐Cysteine and Nanosized Prussian Blue on Gold Electrode for Determination of Human Chorionic Gonadotrophin

et al. 2009

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A novel amperometric immunosensor based on l-cysteine/nanosized Prussian blue bilayer films ({NPB/l-cys} 2 ) and gold nanoparticles (nano-Au) was fabricated for determination of human chorionic gonadotrophin (HCG). First, l-cys and NPB was self-assembled by layer-by-layer (LBL) technology to form {NPB/l-cys} 2 bilayer films on the gold electrode. Subsequently, nano-Au layer was immobilized on the {NPB/l-cys} 2 bilayer films by electrodepositing gold chloride tetrahydrate and then anti-HCG was assembly on the nano-Au layer. Finally hemoglobin (Hb) was employed to block sites against nonspecific binding. With the electrocatalytic ability of Hb and NPB for the reduction of H 2 O 2 , the current signal of the antigen-antibody reaction was amplified and the enhanced sensitivity was achieved. In this study, the assembly process and performance of the immunosensor were characterized by cyclic voltammetry (CV) and the morphology was researched by scanning electron microscopy (SEM). The immunosensor performed a high sensitivity and a wide linear response to HCG in two ranges from 0.5 to 10 mIU/mL and from 10 to 200 mIU/mL with a relatively low detection limit of 0.2 mIU/mL at 3 times the background noise, as well as good stability and long-term life.

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“…Combining with catalytic reaction and immuoreaction [24,25], some high sensitive and selective RS assays were proposed. Nanogold has high electron density, unique optical properties, high biocompatibility, and surface plasmon resonance phenomenon, and was applied to nanoanalysis [26].…”

Section: Introductionmentioning

confidence: 99%

Highly selective resonance scattering detection of trace thrombin using aptamer-modified AuRe nanoprobe

Liang

Jiang

2010

Bioprocess Biosyst Eng

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The gold-rhenium (AuRe) composite nanoparticle was prepared by NaBH(4) reduction procedure, and was modified by the aptamer to obtain an AuRe nanoprobe (AuRessDNA) for thrombin. In pH 7.0 Tris-HCl buffer solution and in the presence of salt, the nanoprobe specifically combined with thrombin to form AuRe-aptamer-thrombin cluster that resulted in the resonance scattering intensity (I (560 nm)) increasing at 560 nm. The increased intensity ΔI (560 nm) was linear to the thrombin concentration in the range of 0.115-6.93 nmol/L, with a regression equation of ΔI (560 nm) = 53.0 C + 2.5, a correlation coefficient of 0.9989, and a detection limit of 13 pmol/L. This method was applied to detect thrombin in human plasma samples, with satisfactory results.

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An immunonanogold resonance scattering spectral probe for rapid assay of human chorionic gonadotrophin

Cited by 29 publications

References 25 publications

Nanocatalytic resonance scattering spectral analysis

Nanocatalytic resonance scattering spectral analysis

An Amperometric Immunosensor Based on Layer‐by‐Layer Assembly of L‐Cysteine and Nanosized Prussian Blue on Gold Electrode for Determination of Human Chorionic Gonadotrophin

Highly selective resonance scattering detection of trace thrombin using aptamer-modified AuRe nanoprobe

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