An immunosensor based on a high performance dual-gate oxide semiconductor thin-film transistor for rapid detection of SARS-CoV-2

Kim, Jingyu; Jeong, Suyeon; Kim, Haneul; Son, Seong Uk; Lee, Seungheon; Rabbani, G.; Kwon, Hyunhwa; Lim, Eun‐Kyung; Ahn, Saeyoung Nate; Park, Sang‐Hee Ko

doi:10.1039/d1lc01116b

Cited by 11 publications

(6 citation statements)

References 29 publications

(33 reference statements)

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“…To our best knowledge, the LOD of 0.14 fg ml −1 is the record sensitive EGFET based S1 protein sensors among literatures. [ 40 ] Moreover, the time‐dependent responses of the 4% device to the increasing concentrations of SARS CoV‐2 spike S1 proteins are shown in Figure S7 (Supporting Information), which indicate that devices have good detection stability, and can be applied to dynamically detect protein concentrations. [ 40 ] However, the devices should be placed in a low‐temperature or nitrogen environment during long‐term storage to avoid oxidation and proteolytic degradation of antibodies (Figure S8, Supporting Information).…”

Section: Resultsmentioning

confidence: 99%

“…[40] Moreover, the time-dependent responses of the 4% device to the increasing concentrations of SARS CoV-2 spike S1 proteins are shown in Figure S7 (Supporting Information), which indicate that devices have good detection stability, and can be applied to dynamically detect protein concentrations. [40] However, the devices should be placed in a low-temperature or nitrogen environment during long-term storage to avoid oxidation and proteolytic degradation of antibodies (Figure S8, Supporting Information). [41] The specificity of the IZO:PEI based biosensors was evaluated by comparing their response of SARS-CoV-2, SARS-CoV-1, and MERS-CoV spike protein in PBS due to their genome similarities.…”

Section: Detection Of Sars-cov-2 Spike S1 Proteinmentioning

confidence: 98%

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Ultrasensitive Detection of SARS‐CoV‑2 by Flexible Metal Oxide Field‐Effect Transistors

Hou,

Wu,

et al. 2023

Adv Funct Materials

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The pandemic of coronavirus disease 2019 (COVID‐19) reflects the great significance of rapid and accurate detection of pathogens by new sensing technologies. Antibody based biosensors with high sensitivity comparable to golden standard polymerase chain reaction (PCR) and miniaturized device features allow the detection of pathogens in portable and flexible formats. Herein, flexible metal oxide electrolyte‐gated field‐effect transistors (EGFETs) are reported to serve as the biosensors for rapid and ultrasensitive severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) detection. The semiconducting layer of the EGFETs associates with hybrid material of PEI doped metal oxides that not only improves the transistor performance, but also regulates microstructure forming higher surface‐to‐volume ratio, which brings more antibodies immobilization, resulting in higher sensitive, and faster response for detecting SARS‐CoV‐2. Comprehensive studies of materials and interfacing engineering of the EGFETs not only build the strong foundation for the EGFET sensors to show excellent sensitivity with a limit of detection from 0.14 fg ml−1 for SARS‐CoV‐2 S1 proteins, and 0.09 copies µl−1 for SARS‐CoV‐2 viruses, but also offer good mechanical properties to enable thin, soft flexible sensing platforms. This work provides a new strategy from materials to devices as innovative schemes for virus/pathogens detection.

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Section: Resultsmentioning

confidence: 99%

Section: Detection Of Sars-cov-2 Spike S1 Proteinmentioning

confidence: 98%

Ultrasensitive Detection of SARS‐CoV‑2 by Flexible Metal Oxide Field‐Effect Transistors

Hou,

Wu,

et al. 2023

Adv Funct Materials

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“…1 While a great deal of effort has gone into developing new ways to treat and prevent the onset of COVID-19, 2−5 it is still affecting many people around the world, and it appears that it will continue to be present for the foreseeable future. 6 At the molecular level, the infection of SARS-CoV-2 is directed via critical interactions between the viral spike protein and human ACE2 protein, 7 catalyzing hydrolysis of the vasoconstrictor peptide angiotensin II. 8,9 To gain a deeper understanding of the interaction observed between SARS-CoV-2 and ACE2, one must first understand the structure of SARS-CoV-2.…”

Section: ■ Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

“…At the molecular level, the infection of SARS-CoV-2 is directed via critical interactions between the viral spike protein and human ACE2 protein, whose normal function is catalyzing hydrolysis of the vasoconstrictor peptide angiotensin II. , To gain a deeper understanding of the interaction observed between SARS-CoV-2 and ACE2, one must first understand the structure of SARS-CoV-2. The overall virus structure consists of a single-stranded RNA genome made up of 30 kilobases that is encapsulated within a lipid bilayer.…”

Section: Introductionmentioning

confidence: 99%

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Label-Free Analysis of Binding and Inhibition of SARS-Cov-19 Spike Proteins to ACE2 Receptor with ACE2-Derived Peptides by Surface Plasmon Resonance

Abouhajar

Chaudhuri

Valiulis

et al. 2022

ACS Appl. Bio Mater.

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SARS-CoV-2 has been shown to enter and infect human cells via interactions between spike protein (S glycoprotein) and angiotensin-converting enzyme 2 (ACE2). As such, it may be possible to suppress the infection of the virus via the blocking of this binding interaction through the use of specific peptides that can mimic the human ACE 2 peptidase domain (PD) α 1-helix. Herein, we report the use of competitive assays along with surface plasmon resonance (SPR) to investigate the effect of peptide sequence and length on spike protein inhibition. The characterization of these binding interactions helps us understand the mechanisms behind peptide-based viral blockage and develop SPR methodologies to quickly screen disease inhibitors. This work not only helps further our understanding of the important biological interactions involved in viral inhibition but will also aid in future studies that focus on the development of therapeutics and drug options. Two peptides of different sequence lengths, [30−42] and [22−44], based on the α 1-helix of ACE2 PD were selected for this fundamental investigation. In addition to characterizing their inhibitory behavior, we also identified the critical amino acid residues of the RBD/ACE2-derived peptides by combining experimental results and molecular docking modeling. While both investigated peptides were found to effectively block the RBD residues known to bind to ACE2 PD, our investigation showed that the shorter peptide was able to reach a maximal inhibition at lower concentrations. These inhibition results matched with molecular docking models and indicated that peptide length and composition are key in the development of an effective peptide for inhibiting biophysical interactions. The work presented here emphasizes the importance of inhibition screening and modeling, as longer peptides are not always more effective.

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Recent Advances in Field‐Effect Transistor‐Based Biosensors for Label‐Free Detection of SARS‐CoV‐2

Yu,

Zhang,

Liu

et al. 2023

Small Science

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Field‐effect transistor‐based biosensors (FET biosensors) have gained significant attention in the context of the global SARS‐CoV‐2 pandemic owing to their label free and highly sensitive detection capabilities. In this comprehensive review, recent advances in the use of electrochemical‐transistor‐based biosensors for the label‐free detection of SARS‐CoV‐2 are analyzed. A general introduction to the electrochemical‐transistor‐based biosensor system is initiated, highlighting its critical technical requirements. Various structural designs and working principles of transistor‐based transducers are described, and the essential aspects of interface engineering for improved sensing performance are summarized. The applications of transistor‐based biosensors for the detection of SARS‐CoV‐2 are discussed in detail. Finally, perspectives for the future development of transistor‐based sensing systems are provided. This review aims to provide valuable insights and guidance for the design and optimization of biochemical sensor systems with the potential to impact health monitoring, disease diagnosis, and biosafety in the field of in vitro diagnostic products.

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An immunosensor based on a high performance dual-gate oxide semiconductor thin-film transistor for rapid detection of SARS-CoV-2

Abstract: We present an immunosensor based on a dual-gate oxide semiconductor thin-film transistor as a platform for detecting SARS-CoV-2.

Cited by 11 publications

References 29 publications

Ultrasensitive Detection of SARS‐CoV‑2 by Flexible Metal Oxide Field‐Effect Transistors

Ultrasensitive Detection of SARS‐CoV‑2 by Flexible Metal Oxide Field‐Effect Transistors

Label-Free Analysis of Binding and Inhibition of SARS-Cov-19 Spike Proteins to ACE2 Receptor with ACE2-Derived Peptides by Surface Plasmon Resonance

Recent Advances in Field‐Effect Transistor‐Based Biosensors for Label‐Free Detection of SARS‐CoV‐2

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