An impedimetric aptasensor for ultrasensitive detection of Penicillin G based on the use of reduced graphene oxide and gold nanoparticles

Mohammad‐Razdari, Ayat; Ghasemi‐Varnamkhasti, Mahdi; Izadi, Zahra; Ensafi, Ali A.; Rostami, Sajad; Siadat, Maryam

doi:10.1007/s00604-019-3510-x

Cited by 46 publications

(17 citation statements)

References 35 publications

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“…MAPA which is bound to PENÀ G molecule with its numerous binding sites introduced by phenylalanine part provides higher sensitivity with lower detection limit to the PENÀ G imprinted MIP-AuNPs nanosensor compared to the other electrochemical sensors [15,16,18,19,20,21,23,24,25,28] have also been reported in the literature. Despite several relevant electrochemical sensors are having better [22,26] limit of detection values, MIP-AuNPs nanosensor showed lower detection limit values when compared to the optic-based sensors (Table 4). [31,32,34,37] Wang et al designed an electrochemical sensor using penicillinase (Pen X)-rhombus porous carbon as the detection element to detect low concentrations of penicillin sodium.…”

Section: The Determination Of Pen-g From Milk Samplementioning

confidence: 99%

“…[25] An impedimetric aptasensor for ultrasensitive detection of Penicillin G based on the use of reduced graphene oxide and gold nanoparticles was prepared by Mohammad et al. [26] Chemiresistive sensors based on nanostructured carbon material (NSCM) are popular for detecting different analytes due to their high sensitivity, low cost and simple construction compared to conventional sensors. [27] Jablonski et al produced Al-p-Si-SiO 2 -Ta 2 O 5 -TMV modified EIS sensor and it is used for Penicillin G detection.…”

Section: The Determination Of Pen-g From Milk Samplementioning

confidence: 99%

“…[11][12][13] Sensors are analytical devices that have a combination of simple and convenient converter receptors containing biological recognition elements. [14] In the last years, electrochemical [15][16][17][18][19][20][21][22][23][24][25][26][27][28][29] and optical sensors based on fluorometric and colorimetric measurements [30][31][32][33] have been used for the estimation of penicillin antibiotics due to their high reactivity and selectivity. Surface plasmon resonance (SPR) is an optical technique based on the intensity of reflected light in a prism covered with a thin metal film.…”

Section: Introductionmentioning

confidence: 99%

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Development of Molecularly Imprinted Polymer‐Based Optical Sensor for the Sensitive Penicillin G Detection in Milk

et al. 2021

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In this study, selective and sensitive Penicillin G (PEN−G) antibiotic detection from both aqueous solution and milk samples using molecular imprinting technique was performed by surface plasmon resonance sensor. For this purpose, PEN−G imprinted poly(2‐Hydroxyethyl methacrylate‐N‐methacroyl‐(L)‐cysteine methyl ester‐gold nanoparticles‐N‐methacryloyl‐L‐phenylalanine methyl ester (MIP‐AuNPs) nanosensor was prepared. Control experiments were carried out via the nanosensor without the addition of AuNPs (MIP) to examine the effect of gold nanoparticles (AuNPs) incorporated to increase the surface plasmon resonance signal response. Moreover, to evaluate the imprinting efficiency, non‐imprinted (NIP‐AuNPs) nanosensor was designed using the same polymerization recipe except the addition of the PEN−G molecule. Characterization studies of MIP, MIP‐AuNPs and NIP‐AuNPs nanosensors were performed with FTIR‐ATR spectrophotometer. Also, MIP‐AuNPs and NIP‐AuNPs nanosensors were characterized by atomic force microscopy, ellipsometer and contact angle measurements. Imprinting efficiency (I.F: 7.83) for the MIP‐AuNPs nanosensor was determined by comparing it with the NIP‐AuNPs nanosensor. The MIP‐AuNPs nanosensor was 9.87 times more selective for the target PEN−G molecule than amoxicillin, and 16.78‐times than ampicillin. In addition, the amount of PEN−G in milk selected as a real sample was measured by spiking 5 ppb PEN−G into the milk.

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Section: The Determination Of Pen-g From Milk Samplementioning

confidence: 99%

Section: The Determination Of Pen-g From Milk Samplementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Development of Molecularly Imprinted Polymer‐Based Optical Sensor for the Sensitive Penicillin G Detection in Milk

et al. 2021

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“…Accordingly, several studies on EIS-based biosensors concentrated on designing aptasensors and immunosensors [ 24 , 25 ]. Aptamers are short single-stranded oligonucleotides (RNA or DNA) with high stability, high accessibility, and strong binding affinity [ 26 ]. Hence, aptamers are perfect for designing high performance EIS biosensors.…”

Section: Impedimetric Biosensorsmentioning

confidence: 99%

Electrochemical Impedance Spectroscopy (EIS): Principles, Construction, and Biosensing Applications

Magar

Hassan

Mulchandani

2021

Sensors

604

238

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Electrochemical impedance spectroscopy (EIS) is a powerful technique used for the analysis of interfacial properties related to bio-recognition events occurring at the electrode surface, such as antibody–antigen recognition, substrate–enzyme interaction, or whole cell capturing. Thus, EIS could be exploited in several important biomedical diagnosis and environmental applications. However, the EIS is one of the most complex electrochemical methods, therefore, this review introduced the basic concepts and the theoretical background of the impedimetric technique along with the state of the art of the impedimetric biosensors and the impact of nanomaterials on the EIS performance. The use of nanomaterials such as nanoparticles, nanotubes, nanowires, and nanocomposites provided catalytic activity, enhanced sensing elements immobilization, promoted faster electron transfer, and increased reliability and accuracy of the reported EIS sensors. Thus, the EIS was used for the effective quantitative and qualitative detections of pathogens, DNA, cancer-associated biomarkers, etc. Through this review article, intensive literature review is provided to highlight the impact of nanomaterials on enhancing the analytical features of impedimetric biosensors.

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“…The concentration of antibiotics in wastewater ranges from a few ng to tens of thousands of μ g . Nonetheless, antibiotics are rarely detected in other environmental media such as air, and the residue in soil mainly comes from irrigation (Mohammad-Razdari et al, 2019a ).…”

Section: Introductionmentioning

confidence: 99%

Low-Dimension Nanomaterial-Based Sensing Matrices for Antibiotics Detection: A Mini Review

Dong

Wang

2020

Front. Chem.

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Antibiotics, a kind of secondary metabolite with antipathogen effects as well as other properties, are produced by microorganisms (including bacterium, fungi, and actinomyces) or higher animals and plants during their lives. Furthermore, as a chemical, an antibiotic can disturb the developmental functions of other living cells. Moreover, it is impossible to avoid its pervasion into all kinds of environmental media via all kinds of methods, and it thus correspondingly becomes a trigger for environmental risks. As described above, antibiotics are presently deemed as a new type of pollution, with their content in media (for example, water, or food) as the focus. Due to their special qualities, nanomaterials, the most promising sensing material, can be adopted to produce sensors with extraordinary detection performance and good stability that can be applied to detection in complicated materials. For low-dimensional (LD) nanomaterials, the quantum size effect, and dielectric confinement effect are particularly strong. Therefore, they are most commonly applied in the detection of antibiotics. This article focuses on the influence of LD nanomaterials on antibiotics detection, summarizes the application of LD nanomaterials in antibiotics detection and the theorem of sensors in all kinds of antibiotics detection, illustrates the approaches to optimizing the sensitivity of sensors, such as mixture and modification, and also discusses the trend of the application of LD nanomaterials in antibiotics detection.

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An impedimetric aptasensor for ultrasensitive detection of Penicillin G based on the use of reduced graphene oxide and gold nanoparticles

Cited by 46 publications

References 35 publications

Development of Molecularly Imprinted Polymer‐Based Optical Sensor for the Sensitive Penicillin G Detection in Milk

Development of Molecularly Imprinted Polymer‐Based Optical Sensor for the Sensitive Penicillin G Detection in Milk

Electrochemical Impedance Spectroscopy (EIS): Principles, Construction, and Biosensing Applications

Low-Dimension Nanomaterial-Based Sensing Matrices for Antibiotics Detection: A Mini Review

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