An impedimetric DNA sensor based on functionalized magnetic nanoparticles for HIV and HBV detection

Hassen, Walid; Chaix, Carole; Abdelghani, Adnane; Bessueille, François; Léonard, Didier; Jaffrézic‐Renault, Nicole

doi:10.1016/j.snb.2008.06.020

Cited by 118 publications

(51 citation statements)

References 19 publications

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“…Many types of nanoparticles of different sizes and compositions are now available, which facilitates their application in electroanalysis. Different kinds of nanoparticles, and sometimes the same kind of nanoparticles, can play different roles in different electrochemical sensing systems, such as enzyme sensors, immunosensors and DNA sensors [2][3][4][5]. Generally, metal nanoparticles have excellent conductivity and catalytic properties, which make them suitable for acting as ''electronic wires" to enhance the electron transfer between redox centers in proteins and electrode surfaces and as catalysts to increase electrochemical reactions.…”

Section: Introductionmentioning

confidence: 99%

Selective voltammetric determination of d-penicillamine in the presence of tryptophan at a modified carbon paste electrode incorporating TiO2 nanoparticles and quinizarine

Mazloum-Ardakani¹,

Beitollahi²,

Taleat³

et al. 2010

Journal of Electroanalytical Chemistry

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Section: Introductionmentioning

confidence: 99%

Selective voltammetric determination of d-penicillamine in the presence of tryptophan at a modified carbon paste electrode incorporating TiO2 nanoparticles and quinizarine

Mazloum-Ardakani¹,

Beitollahi²,

Taleat³

et al. 2010

Journal of Electroanalytical Chemistry

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“…There have been various electrochemical genomagnetic sensor technology for monitoring of spesific DNA hybridization [63][64][65][66][67][68]. A genomagnetic assay coupling of electrochemical monitoring was studied for the detection of wild type Hepatitis B virus (HBV) DNA in polymerase chain reaction (PCR) amplicons by using PGE and DPV.…”

Section: Magnetic Particlesmentioning

confidence: 99%

“…by using graphite-epoxy composite (GEC) and magneto-GEC electrodes with DPV technique based on the changes in the oxidation signal of guanine [64]. Hassen et al presented the genomagnetic assay based on DNA hybridization by measuring guanine oxidation signal for detecting Human Immunodeficiency virus (HIV) and HBV virus using CV and nonfaradic electrochemical impedance spectroscopy (EIS) with gold electrode (AuE) [65]. The detection of DNA hybridization in connection to cadmium sulfide nanoparticle tracers and electrochemical stripping measurements of the cadmium oxidation signal was performed by thin mercury-film electrode [66].…”

Section: Magnetic Particlesmentioning

confidence: 99%

The Recent Electrochemical Biosensor Technologies for Monitoring of Nucleic Acid Hybridization

Karadeniz¹,

Kuralay²,

Abacı³

et al. 2011

CAC

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Electrochemical biosensors have a key importance in many areas including clinical, biological, pharmaceutical, forensic, environmental and agricultural applications. They have the advantages of rapid, easy and low cost preparation allowing the localization of biocompound on electrodes of any size with independence of sample turbidity, high sensitivity and high selectivity. Electrochemical devices have attracted considerable interest in the development of DNA hybridization biosensors. They rely on the conversion of hybridization event into a direct electrochemical signal. Many efforts have been carried out in the detection of DNA hybridization for improving the stability, reproducibility and sensitivity.This review summarizes the advanced electrochemical methodologies involved in nucleic acid hybridization performed by using polymer technology, nanoparticles (NPs), magnetic particles, and carbon nanotubes (CNTs). The preparation of polymer modified electrodes especially including conducting polymers and their applications for DNA hybridization are given with the advantages of electrocatalysis, good electroactivity, high sensitivity and providing suitable matrix for DNA immobilization. The role of various nanomaterials in biosensors with the advantages of high surface area, fast heterogeneous electron transfer, excellent biocompatibility and enhanced signal with a good selectivity are presented. The combination of these techniques is also discussed in details.

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“…The unique combination of high magnetization and paramagnetic behavior opens these materials to a very wide range of applications. Greater availability of the surface area facilitates in biosensor [15], drug delivery [16] and tissue repair [17], MRI [18], hyperthermic effect [19], cell tagging and tracking [20], gene delivery [21,22], detection of probes [23,24], in agriculture, biosensor, rapid separation in environmental biology and concentration tracing of specific targets, such as bacteria, leukocyte and protein [25]. The metallic nanoparticles are most promising as they contain antibacterial properties due to their large ratio betwwen surface area and volume.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Antimicrobial Activity of Biosynthesized Iron and Silver Nanoparticles Using the Fungi Fusarium Oxysporum and Actinomycetes SP. on Human Pathogens

Abdeen¹,

Isaac²,

Geo³

et al. 2013

Nano BioMed ENG

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A simple and reproducible biosynthetic method was employed to synthesize iron and silver nanoparticles which resulted in monodispersed nanoparticles of high concentration. The iron oxide nanoparticles has been widely favored because of low cytotoxicity, biodegradable and reactive surface that can be modified with biocompatible coatings. Silver nanoparticles have been a potent antibacterial, antifungal, anti-viral and antiinflammatory agent. The reaction process was simple, eco-friendly, inexpensive and easy to handle. Green and chemical methods were employed to synthesize iron and silver nanoparticles. A microbial route to synthesize iron and silver nanoparticles by the fungal strain Fusarium oxysporum sp. and Actinomycetes sp. was done simultaneously. Production of nanoparticles using fungi has some advantages over other organisms as it is easy to handle and require simple raw materials. The obtained iron and silver nanoparticles were characterized by UV-vis spectroscopy, Fourier transform infrared spectroscopy (FTIR) and the morphology of prepared nanoparticles was confirmed by Transmission electron microscopy (TEM). TEM images of Iron nanoparticles synthesized by Fusarium oxysporum sp. showed 20-40 nm sized particles. These particles exhibited maximum antibacterial activity against Bacillus, E. coli and Staphylococcus sps. TEM images of biosynthesized silver nanoparticles were of smaller size (10-20 nm). The microbially synthesized silver nanoparticles using Actinomycetes were found to be highly toxic against different human pathogens due to the smaller size and due to the presence of antibiotic components available on them. The mechanism of antimicrobial property of nanoparticle lies with the fact that the extremely small size means a large surface area relative to the volume, which effectively covers the microorganisms and reduce oxygen supply for respiration. It was found that silver nanoparticles synthesized by the microbial route have a greater antibacterial activity.Keywords: Iron nanoparticles, silver nanoparticles, Phyllanthus emblica, Fusarium oxysporum sp., Actinomycetes sp., microbial synthesis Citation: A. Sunitha, et al. EEvaluation of antimicrobial activity of biosynthesized iron and silver Nanoparticles using the fungi Fusarium oxysporum and Actinomycetes sp. on human pathogens.

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An impedimetric DNA sensor based on functionalized magnetic nanoparticles for HIV and HBV detection

Cited by 118 publications

References 19 publications

Selective voltammetric determination of d-penicillamine in the presence of tryptophan at a modified carbon paste electrode incorporating TiO2 nanoparticles and quinizarine

Selective voltammetric determination of d-penicillamine in the presence of tryptophan at a modified carbon paste electrode incorporating TiO2 nanoparticles and quinizarine

The Recent Electrochemical Biosensor Technologies for Monitoring of Nucleic Acid Hybridization

Evaluation of Antimicrobial Activity of Biosynthesized Iron and Silver Nanoparticles Using the Fungi Fusarium Oxysporum and Actinomycetes SP. on Human Pathogens

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