An impedimetric study of DNA hybridization on paper-supported inkjet-printed gold electrodes

Abstract

In this study, two different supramolecular recognition architectures for impedimetric detection of DNA hybridization have been formed on disposable paper-supported inkjet-printed gold electrodes. The gold electrodes were fabricated using a gold nanoparticle based ink. The first recognition architecture consists of subsequent layers of biotinylated self-assembly monolayer (SAM), streptavidin and biotinylated DNA probe. The other recognition architecture is constructed by immobilization of thiol-functionalized DNA probe (HS-DNA) and subsequent backfill with 11-mercapto-1-undecanol (MUOH) SAM. The binding capacity and selectivity of the recognition architectures were examined by surface plasmon resonance (SPR) measurements. SPR results showed that the HS-DNA/MUOH system had a higher binding capacity for the complementary DNA target. Electrochemical impedance spectroscopy (EIS) measurements showed that the hybridization can be detected with impedimetric spectroscopy in picomol range for both systems. EIS signal indicated a good selectivity for both recognition architectures, whereas SPR showed very high unspecific binding for the HS-DNA/MUOH system. The factors affecting the impedance signal were interpreted in terms of the complexity of the supramolecular architecture. The more complex architecture acts as a less ideal capacitive sensor and the impedance signal is dominated by the resistive elements.

Progress of alternative sintering approaches of inkjet-printed metal inks and their application for manufacturing of flexible electronic devices

“…77,156 Ihalainen et al have successfully manufactured a paper based hygroscopic insulator eld effect transistor (HIFET), consisting of Ag source and drain electrodes, polythiophene (PQT) as a semiconductor layer, poly(4-vinylphenol) as an insulating layer and PEDOT:PSS as a gate electrode. 77,156 Ihalainen et al have successfully manufactured a paper based hygroscopic insulator eld effect transistor (HIFET), consisting of Ag source and drain electrodes, polythiophene (PQT) as a semiconductor layer, poly(4-vinylphenol) as an insulating layer and PEDOT:PSS as a gate electrode.…”

“…1b). 48,[76][77][78] Further reported applications employing printed metal patterns include photodetectors (Fig. 1c).…”

Low-cost bioanalysis on paper-based and its hybrid microfluidic platforms

“…Ihalainen et al . [100] developed two different supramolecular recognition architectures for impedimetric detection of DNA hybridization on paper-based inkjet-printed gold electrodes. The first architecture comprises alternating layers of biotinylated self-assembly monolayer (SAM), streptavidin and the biotinylated DNA probe.…”

On the Slow Diffusion of Point-of-Care Systems in Therapeutic Drug Monitoring

“…Carbon nanotubes, for example, effectively provide enhancement of electrodes transduction capabilities, especially coupled with electrochemical detection of the analyte (West and Halas, 2003). Different configurations, designed to be soon compatible with implantable devices, have been published able to recognize and quantify anti-inflammatory drug like naproxene, in spiked serum sample at clinically relevant sensitivity (Popovtzer et al, 2006; Alonso-Lomillo et al, 2010; Pruneanu et al, 2011; Cruz et al, 2014; Ihalainen et al, 2014). This design, for example, is very versatile, as it exploits enzymes involved in hepatic drug metabolism as receptor elements.…”