An implantable and controlled drug-release silk fibroin nanofibrous matrix to advance the treatment of solid tumour cancers

Xie, Maobin; Fan, Dejun; Chen, Yufeng; Zhao, Zheng; He, Xiaowen; Li, Gang; Chen, Ai-Zheng; Wu, Xiaojian; Li, Jiashen; Li, Zhi; Hunt, John A.; Li, Yang; Lan, Ping

doi:10.1016/j.biomaterials.2016.06.049

Cited by 58 publications

(34 citation statements)

References 27 publications

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“…Figure B shows silk fibroin nanofibrous‐based drug delivery nanosystem for cancer treatment. The silk fibroin nanofibrous with controllable fiber diameter loaded with curcumin displays a superior anticancer potential in colorectal cancer cells at a concentration of greater than 5 µg mL −1 . In addition to bioengineered spider silk spheres and silk fibroin nanofibrous, sericin nanoparticles can also serve as a targeted drug‐delivery platform.…”

Section: Multifunctional Applicationsmentioning

confidence: 99%

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Engineering the Future of Silk Materials through Advanced Manufacturing

et al. 2018

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Silk is a natural fiber renowned for its outstanding mechanical properties that have enabled the manufacturing of ultralight and ultrastrong textiles. Recent advances in silk processing and manufacturing have underpinned a re-interpretation of silk from textiles to technological materials. Here, it is argued that silk materials-optimized by selective pressure to work in the environment at the biotic-abiotic interface-can be harnessed by human micro- and nanomanufacturing technology to impart new functionalities and opportunities. A critical overview of recent progress in silk technology is presented with emphasis on high-tech applications enabled by recent innovations in multilevel modifications, multiscale manufacturing, and multimodal characterization of silk materials. These advances have enabled successful demonstrations of silk materials across several disciplines, including tissue engineering, drug delivery, implantable medical devices, and biodissolvable/degradable devices.

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Section: Multifunctional Applicationsmentioning

confidence: 99%

“…B) Illustration of curcumin‐loaded silk fibroin nanofibrous matrix for smart cancer treatment. Reproduced with permission . Copyright 2016, Elsevier Ltd. C) Synthesis procedure of DOX‐loaded sericin‐coated MSNs.…”

Section: Multifunctional Applicationsmentioning

confidence: 99%

Engineering the Future of Silk Materials through Advanced Manufacturing

et al. 2018

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“…The chemical structure of CM-SF NPs was confirmed by FTIR results; it is believed that the incorporated CM most likely interacted with the hydrophobic residues in the silk nanoplatforms. 22,26 Moreover, the 3,495 cm -1 (O-H) peak in CM shifted to 3,290 cm -1 , suggesting that CM linked to the SF nanoplatforms, maybe via hydrogen bonds. 19 Importantly, the solubility of CM-SF NPs was greatly improved, which was mainly due to size reduction, supersaturation of CM, water solubility of the SF nanoplatform, better dispersibility and wettability of CM, and hydrogen bonds between CM and the SF nanoplatform.…”

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confidence: 95%

“…Procedures were based on our previous studies. 13,22 First, 5×10 4 HCT116 cells were seeded onto coverslips in 24-well plates for cell attachment. Then, cells were treated with samples for 0.5 and 6 hours.…”

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confidence: 99%

Supercritical carbon dioxide-developed silk fibroin nanoplatform for smart colon cancer therapy

Xie¹,

Fan²,

Li³

et al. 2017

IJN

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Purpose To deliver insoluble natural compounds into colon cancer cells in a controlled fashion. Materials and methods Curcumin (CM)–silk fibroin (SF) nanoparticles (NPs) were prepared by solution-enhanced dispersion by supercritical CO 2 (SEDS) (20 MPa pressure, 1:2 CM:SF ratio, 1% concentration), and their physicochemical properties, intracellular uptake efficiency, in vitro anticancer effect, toxicity, and mechanisms were evaluated and analyzed. Results CM-SF NPs (<100 nm) with controllable particle size were prepared by SEDS. CM-SF NPs had a time-dependent intracellular uptake ability, which led to an improved inhibition effect on colon cancer cells. Interestingly, the anticancer effect of CM-SF NPs was improved, while the side effect on normal human colon mucosal epithelial cells was reduced by a concentration of ~10 μg/mL. The anticancer mechanism involves cell-cycle arrest in the G 0 /G 1 and G 2 /M phases in association with inducing apoptotic cells. Conclusion The natural compound-loaded SF nanoplatform prepared by SEDS indicates promising colon cancer-therapy potential.

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“…Presently, many research groups work to enhance the solubility and pharmacokinetic property of native curcumin by encapsulation through either a mechanical or a chemical process. The mechanical process utilizes the solution-enhanced dispersion technique by supercritical CO 2 to produce curcumin nanoparticles 34,35. Although the method is able to manipulate the properties of the obtained nanoparticles by controlling the parameters of the synthesis process including the flow rate of curcumin solution, precipitation pressure, concentration of curcumin solution, and precipitation temperature, the experimental setups are complicated, and the products are not always in aqueous forms.…”

Section: Introductionmentioning

confidence: 99%

Folic acid-conjugated amphiphilic alternating copolymer as a new active tumor targeting drug delivery platform

Szewczuk

Malardier-Jugroot

2016

DDDT

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Targeted drug delivery using polymeric nanostructures is an emerging cancer research area, engineered for safer, more efficient, and effective use of chemotherapeutic drugs. A pH-responsive, active targeting delivery system was designed using folic acid functionalized amphiphilic alternating copolymer poly(styrene-alt-maleic anhydride) (FA-DABA-SMA) via a biodegradable linker 2,4-diaminobutyric acid (DABA). The polymeric template is pH responsive, forming amphiphilic nanostructures at pH 7, allowing the encapsulation of hydrophobic drugs on its interior. Moreover, the structure is stable only at neutral pH and collapses in the acidic tumor microenvironment, releasing drugs on-site from its core. The delivery vehicle is investigated using human pancreatic PANC-1 cancer cells and RAW-Blue™ mouse macrophage reporter cell line, both of which have overly expression of folic acid receptors. To trace the cellular uptake by both cell lines, curcumin was selected as a dye and drug mimic owing to its fluorescence nature and hydrophobic properties. Fluorescent microscopy of FA-DABA-SMA loaded with curcumin revealed a significant internalization of the dye by human pancreatic PANC-1 cancer cells compared to those with unfunctionalized polymers (SMA). Moreover, the FA-DABA-SMA polymers exhibit rodlike association specific to the cells. Both empty SMA and FA-DABA-SMA show little toxicity to PANC-1 cells as characterized by WST-1 cell proliferation assay. These results clearly indicate that FA-DABA-SMA polymers show potential as an active tumor targeting drug delivery system with the ability to internalize hydrophobic chemotherapeutics after they specifically attach to cancer cells.

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An implantable and controlled drug-release silk fibroin nanofibrous matrix to advance the treatment of solid tumour cancers

Cited by 58 publications

References 27 publications

Engineering the Future of Silk Materials through Advanced Manufacturing

Engineering the Future of Silk Materials through Advanced Manufacturing

Supercritical carbon dioxide-developed silk fibroin nanoplatform for smart colon cancer therapy

Folic acid-conjugated amphiphilic alternating copolymer as a new active tumor targeting drug delivery platform

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